A First Generation Microsatellite- and SNP-Based Linkage Map of Jatropha

Wang, C. M.; Liu, Peng; Che, Yi; Gu, Ke; Sun, Fei; Li, Lei; Lo, Loong Chueng; Liu, Xiaokun; Feng, Felicia; Lin, Grace; Cao, Suying; Hong, Yan; Zhen, Yin; Yue, Gen Hua

doi:10.1371/journal.pone.0023632

Cited by 70 publications

(58 citation statements)

References 33 publications

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“…One reason for this can be an inherent feature of the microsatellite regions in the genome of jatropha. Wang et al (2011) observed low genetic variation in SSR regions of the jatropha genome among different genotypes. Similar to our study, Mukherjee et al (2011) reported a monomorphic amplification pattern for most of the SSR markers used to study the genome of jatropha genotypes.…”

Section: Genetic Diversity Evaluation By Molecular Markersmentioning

confidence: 88%

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Santos

Ferreira

Setotaw

et al. 2016

An. Acad. Bras. Ciênc.

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Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica (47), Mexican accessions (42, 43, 44 and 45) and some national accessions (28, 29, 41 and 46). Therefore, aiming to increase the genetic variability and improve the effectiveness of jatropha breeding programs, it is suggested to explore such as parental accessions to generate commercial hybrids. This fact implies the possibility to support future production of jatropha, since this culture may be an important source of income, especially for small farmers living in semiarid regions of Brazil.

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Section: Genetic Diversity Evaluation By Molecular Markersmentioning

confidence: 88%

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Santos

Ferreira

Setotaw

et al. 2016

An. Acad. Bras. Ciênc.

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“…Penelitian ini menghasilkan peta genetik jarak pagar sepanjang 1.655,8 cM dengan rerata densitas marka 1,4 cM (Wu et al 2015). Peta genetik ini disusun pada 11 LG yang sama dengan jumlah kromosom jarak pagar dengan penomoran kromosom persis sama seperti penomoran pada peta genetik sebelumnya (King et al 2013;Wang et al 2011). …”

Section: Peta Genetik Untuk Pemetaan Gen/qtl Karakter Unggulunclassified

“…Ketersediaan tiga peta genetik jarak pagar (yang juga mengandung synteny genom jarak kepyar) hasil konstruksi tiga kelompok peneliti yang berbeda (King et al 2013;Wang et al 2011;Wu et al 2015) menyediakan fondasi yang kuat dan sangat penting bagi pemulia tanaman jarak pagar untuk mengidentifikasi dan melabel gen dan QTL karakter-karakter bernilai ekonomis tinggi, di antaranya karakter komponen hasil, pembungaan, dan panen buah serempak untuk menurunkan biaya panen; rasio bunga betina dan bunga jantan yang tinggi sehingga produktivitas biji meningkat; bijinya nontoksik sehingga ampas biji jarak pagar setelah diperas minyaknya dapat langsung digunakan sebagai pakan ternak; kadar dan mutu minyak yang tinggi sehingga meningkatkan kualitas biodiesel; karakter penting lainnya, seperti ketahanan terhadap hama dan penyakit utama, juga toleransi terhadap cekaman lingkungan, seperti kekeringan dan keracunan Al.…”

Section: Peta Genetik Untuk Pemetaan Gen/qtl Karakter Unggulunclassified

Pendekatan Bioteknologi dan Genomika untuk Perbaikan Genetik Tanaman Jarak Pagar sebagai Penghasil Bahan Bakar Nabati

Tasma

2018

J. AgroBiogen

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Physic nut (Jatropha curcas L.) is an oil-bearing crop whose oil can be used as diesel substitution. This crop can be grown at suboptimal soils and thus attracts many people to explore its potential as a biorenewable energy crop. Many constrains, however, are faced by this crop. From plant material and cultivation aspects, not much information has yet been known regarding this crop. In addition, people still view physic nut as an incomplete domesticated crop as indicated by the fact that many J. curcas accessions of the world still have toxic in their seeds causing the protein-rich seeds can not directly be used as animal feed. J. curcas demonstrates continuous fruiting habit during its fruiting season and hence no uniform fruit maturity causing expensive harvesting cost. The female and male flower ratio is low causing lower seed productivity. J. curcas oil is relatively rich with poly unsaturated fatty acids that need to be decreased to improve diesel quality. Genomic knowledge to understand J. curcas genes and function is critical to manipulate J. curcas genetics systematically. Genetic transformation technology is potentially applied for better plant architecture, improvement of agronomically important characters, seed and oil yield, and quality traits. The objective of this manuscript was to review biotechnology and genomic approaches for genetic improvement of J. curcas plant materials. The biotechnological method application should expedite J. curcas breeding programs. With superior planting materials, the J. curcas should be able to be cultivated economically and resulting better J. curcas oil suitable for biodiesel industry.Keywords: Jatropha curcas, genomics, gene and QTL, genetic transformation, marker-assisted breeding. ABSTRAKJarak pagar (Jatropha curcas L.) merupakan tanaman penghasil minyak nabati yang dapat digunakan sebagai pengganti minyak diesel. Tanaman yang dapat tumbuh pada kondisi lahan kurang subur ini menarik minat banyak pihak untuk mengekplorasi potensinya sebagai tanaman sumber energi yang ramah lingkungan. Namun, masih banyak kendala yang dihadapi dalam pembudidayaannya supaya dapat diusahakan secara ekonomis. Dari aspek bahan tanaman dan budi daya, saat ini tanaman jarak pagar masih belum banyak diketahui. Bahkan, jarak pagar masih dianggap sebagai tanaman yang belum didomestikasikan secara penuh seperti ditunjukkan oleh fakta bahwa sebagian besar genotipe jarak pagar di dunia bijinya toksik sehingga ampas bijinya yang kaya protein tidak dapat langsung digunakan sebagai pakan ternak. Kematangan buah tanaman ini tidak serempak yang menyebabkan biaya panen tinggi. Rasio bunga betina dan bunga jantan yang rendah menyebabkan produktivitas bijinya rendah. Biji jarak pagar mengandung asam lemak poli tidak jenuh yang konsentrasinya perlu diturunkan untuk meningkatkan mutu minyak diesel. Pengetahuan genomika memungkinkan untuk mengetahui komposisi genom, komposisi dan fungsi gen, dan pemetaan genetik (gen/QTL) unggul jarak pagar. Pemahaman ini diperlukan agar genetika tanaman jarak...

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“…However, Jatropha has a short history of breeding and is still essentially a wild plant; therefore, improved cultivars with stable yields in fluctuating environments remain to be developed. With the development of molecular breeding technology, various kinds of PCR-based molecular markers have been established for Jatropha, such as random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), inter simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) (Basha and Sujatha, 2007;Sudheer Pamidimarri et al, 2009b;Wang et al, 2011;Kanchanaketu et al, 2012;Sillma et al, 2016). These technologies have the potential to assist in rapid breeding advances in Jatropha.…”

Section: Introductionmentioning

confidence: 99%

Nuclear isolation and purification using SDS/urea (NIPSU) method for efficient and rapid extraction of high-purity genomic DNAs from Jatropha curcas L: A comparative analysis of DNA isolation protocols

Nanasato¹,

Uenoyama²,

Tomooka³

et al. 2018

Afr. J. Biotechnol.

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Jatropha curcas L. (Jatropha) has gained popularity for its potential use in biodiesel production in arid regions, and its yield improvement by molecular breeding has been anticipated. However, Jatropha is known for its recalcitrance in the extraction of pure genomic DNAs. In this study, four DNA extraction methods were comparatively examined for their efficiency and quality of genomic DNA isolations from Jatropha. Consequently, one method, designated as nuclear isolation and purification using SDS/urea (NIPSU) method was found to be the most effective for rapid preparation of high yield and quality genomic DNA from Jatropha leaves. NIPSU method allowed extraction of genomic DNAs from leaves, stems, and roots tissues of Jatropha in high yield (6.8 to 24.5 µg gFW -1 ), with spectroscopically high purity (A 260 /A 280 and A 260 /A 230 >1.8). NIPSU method was also applicable to the dehydrated leaves for genomic DNA preparations. The isolated genomic DNA was amenable for downstream molecular procedures such as polymerase chain reaction, restriction enzyme cutting and Southern blotting analyses.

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A First Generation Microsatellite- and SNP-Based Linkage Map of Jatropha

Cited by 70 publications

References 33 publications

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Pendekatan Bioteknologi dan Genomika untuk Perbaikan Genetik Tanaman Jarak Pagar sebagai Penghasil Bahan Bakar Nabati

Nuclear isolation and purification using SDS/urea (NIPSU) method for efficient and rapid extraction of high-purity genomic DNAs from Jatropha curcas L: A comparative analysis of DNA isolation protocols

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