A first case of New Delhi metallo-β-lactamase-7 in an Escherichia coli ST648 isolate in Japan

Mizuno, Yasutaka; Yamaguchi, Tetsuo; Matsumoto, Tetsuya

doi:10.1016/j.jiac.2014.08.009

Cited by 21 publications

(11 citation statements)

References 14 publications

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“…All of the isolates were positive for bla NDM-7 according to PCR and sequencing results ( Table 2). Enterobacteriaceae with bla NDM-7 are relatively uncommon, having only been previously described in E. coli from France (26), Germany (27), India (28), the United States (29), and Japan (30) and in K. pneumoniae from the United States (29), Spain (31), and Denmark (32). The single biggest risk factor in all patients was a history of recent travel to India with two exceptions; the patient in the French case had previously traveled to Burma, while no connection with the Indian subcontinent was established among the Spanish patients (26,31).…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Chen

Peirano

Lynch

et al. 2016

Antimicrob Agents Chemother

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g Enterobacteriaceae with bla NDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring bla NDM-7 . To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with bla NDM-7 .

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Chen

Peirano

Lynch

et al. 2016

Antimicrob Agents Chemother

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“… 9 Despite the fact that about half of the isolates came from India, the study established the presence of NDM-producing organisms in other parts of the world, such as Vietnam, Serbia, the Philippines, the Middle East, Guatemala, and the USA. Reports of NDM-producing organisms have also surfaced from Pakistan, 10 the UK, 10 Japan, 11 the Netherlands, 12 and Australia. 13 Figure 1 depicts the geographical distribution of NDM; the areas where NDM producers are most commonly isolated are located on the Asian continent.…”

Section: The New Delhi Metallo-beta-lactamase Carbapenemasementioning

confidence: 99%

Impact of the New Delhi metallo-beta-lactamase on beta-lactam antibiotics

Zmarlicka

Nailor

Nicolau

2015

IDR

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Since the first New Delhi metallo-beta-lactamase (NDM) report in 2009, NDM has spread globally causing various types of infections. NDM-positive organisms produce in vitro resistance phenotypes to carbapenems and many other antimicrobials. It is thus surprising that the literature examining clinical experiences with NDM does not report corresponding poor clinical outcomes. There are many instances where good clinical outcomes are described, despite a mismatch between administered antimicrobials and resistant in vitro susceptibilities. Available in vitro data for either monotherapy or combination therapy does not provide an explanation for these observations. However, animal studies do begin to shed more light on this phenomenon. They imply that the in vivo expression of NDM may not confer clinical resistance to all cephalosporin and carbapenem antibiotics as predicted by in vitro testing but other resistance mechanisms need to be present to generate a resistant phenotype. As such, previously abandoned therapies, particularly carbapenems and beta-lactamase inhibitor combinations, may retain utility against infections caused by NDM producers.

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“…As a type of β-lactam antibiotic, carbapenems are generally used as the last resort against lethal infections by multidrug-resistant Gram-negative pathogens (3). Unfortunately the emergence of the notorious New Delhi β-lactamases (NDM-1 [3] and its variants NDM-5 [4], NDM-7 [5], and NDM-9 [6]) in diversified species of Enterobacteriaceae (e.g., Escherichia coli and Klebsiella pneumoniae ) has almost pushed us to the cusp of the postantibiotic era. To conquer the carbapenemase (and/or extended-spectrum β-lactamase [ESBL])-producing pathogens, the cationic polypeptide antibiotic colistin (also referred to as polymyxin E; see Fig.…”

Section: Introductionmentioning

confidence: 99%

Deciphering MCR-2 Colistin Resistance

Sun

Gao

et al. 2017

mBio

129

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Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered a final resort against lethal infections by multidrug-resistant bacteria. Colistin is a cationic polypeptide antibiotic and acts as the last line of defense for treatment of carbapenem-resistant bacteria. Very recently, a new plasmid-borne colistin resistance gene, mcr-2, was revealed soon after the discovery of the paradigm gene mcr-1, which has disseminated globally. However, the molecular mechanisms for MCR-2 colistin resistance are poorly understood. Here we show a unique transposon unit that facilitates the acquisition and transfer of mcr-2. Evolutionary analyses suggested that both MCR-2 and MCR-1 might be traced to their cousin phosphoethanolamine (PEA) lipid A transferase from a known polymyxin producer, Paenibacillus. Transcriptional analyses showed that the level of mcr-2 transcripts is relatively higher than that of mcr-1. Genetic deletions revealed that the transmembrane regions (TM1 and TM2) of both MCR-1 and MCR-2 are critical for their location and function in bacterial periplasm, and domain swapping indicated that the TM2 is more efficient than TM1. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) confirmed that all four MCR proteins (MCR-1, MCR-2, and two chimeric versions [TM1-MCR-2 and TM2-MCR-1]) can catalyze chemical modification of lipid A moiety anchored on lipopolysaccharide (LPS) with the addition of phosphoethanolamine to the phosphate group at the 4′ position of the sugar. Structure-guided site-directed mutagenesis defined an essential 6-residue-requiring zinc-binding/catalytic motif for MCR-2 colistin resistance. The results further our mechanistic understanding of transferable colistin resistance, providing clues to improve clinical therapeutics targeting severe infections by MCR-2-containing pathogens.

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A first case of New Delhi metallo-β-lactamase-7 in an Escherichia coli ST648 isolate in Japan

Cited by 21 publications

References 14 publications

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Impact of the New Delhi metallo-beta-lactamase on beta-lactam antibiotics

Deciphering MCR-2 Colistin Resistance

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A first case of New Delhi metallo-β-lactamase-7 in an Escherichia coli ST648 isolate in Japan

Cited by 21 publications

References 14 publications

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla NDM-7 in Enterobacteriaceae from Calgary, Canada

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla NDM-7 in Enterobacteriaceae from Calgary, Canada

Impact of the New Delhi metallo-beta-lactamase on beta-lactam antibiotics

Deciphering MCR-2 Colistin Resistance

Contact Info

Product

Resources

About

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada