A first attempt to evaluate the toxicity to Phaeodactylum tricornutum Bohlin exposed to rare earth elements

Siciliano, Antonietta; Sabatino, Manuela; Paone, Antonietta; Suarez, Edith Guadalupe Padilla; Toscanesi, Maria; Brouziotis, Antonios Apostolos; Gambino, Edvige; Saviano, Lorenzo; Trifuoggi, Marco; Guida, Marco; Libralato, Giovanni

doi:10.3389/fenvs.2022.957943

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…Here, the nominal EC 20 and EC 50 obtained from standard 72 h exposure were used for the long-term exposure of P. tricornutum to Ce or Gd. These obtained values were for both Ce (2.39 mg/L for EC 20 , and 3.13 for EC 50 ) and Gd (4.52 mg/L for EC 20 , and 6.02 for EC 50 ) higher than those previously obtained by Siciliano et al [26].…”

Section: Discussioncontrasting

confidence: 61%

“…REE tolerance mechanisms have been reported in plants such as Dicranopteris linearis, Phytolacca sp., Helianthus Annuus [32][33][34], and in microalgae such as Galdieria sulphuraria and Desmodesmus quadricauda [35,36], but very little information concerns diatoms [26].…”

Section: Discussionmentioning

confidence: 99%

“…While specific studies on P. tricornutum are limited [26], drawing upon general knowledge of organisms' responses to chemical compounds, it is plausible to speculate that P. tricornutum may possess tolerance or adaptation strategies to cope with exposure to cerium (Ce) or gadolinium (Gd), just as observed in some microalgae [13,39,40]. However, Gd showed a more evident effect on P. tricornutum.…”

Section: Discussionmentioning

confidence: 99%

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Multi-Endpoint Analysis of Cerium and Gadolinium Effects after Long-Term Exposure to Phaeodactylum tricornutum

Siciliano,

Spampinato,

Salbitani

et al. 2024

Environments

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The significantly increasing levels of Rare Earth Elements (REEs) in seawater are largely due to multiple anthropogenic activities. Their effects on marine primary producers such as Phaeodactylum tricornutum have not been fully assessed. This study focused on examining the long-term impacts of these two commonly occurring REEs, cerium (Ce) and gadolinium (Gd), on marine diatoms by 28 d of exposure. The 72 h effective concentrations that inhibited the growth of 20% (EC20) and 50% (EC50) of the exposed population were used for long-term exposures. The growth, oxidative stress level, photosynthetic pigments, and chlorophyll fluorescence were assessed in the diatoms, after 7, 14, 21, and 28 d of REEs exposure. Results display a difference in the toxicity induced by the two elements. Exposure to 2.39 mg/L (EC20) and 3.13 mg/L (EC50) of Ce, and to 4.52 mg/L (EC20) and 6.02 mg/L (EC50) of Gd displayed a lower effect on the growth of algae cells, as the response remained below 20% for inhibition or stimulation. Except for GD, the ROS and the activities of SOD, and LPO showed, during the exposure, comparable levels respect to control cells. A change in chlorophyll levels was also observed especially under Ce exposure. Both elements showed changes in photosynthetic performance. This study provides new insights into the different effects of Ce and Gd on P. tricornutum, demonstrating their diverse modes of action on this important primary producer. The findings provide further evidence of the adverse effects of anthropogenic REEs pollution on marine ecosystems.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Multi-Endpoint Analysis of Cerium and Gadolinium Effects after Long-Term Exposure to Phaeodactylum tricornutum

Siciliano,

Spampinato,

Salbitani

et al. 2024

Environments

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“…For example, high levels (in the ng L −1 range) of gadolinium, from gadolinium-based contrast agents, have been frequently documented in aquatic environments in developed countries, and lanthanum and samarium have been detected in the river Rhine at alarmingly high concentrations [ 11 ]. Therefore, REEs are considered in all respects a new category of emerging contaminants, and public awareness and concern are gradually increasing [ 3 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr3+, Eu3+, Ho3+, Tm3+)

Salvatore

Siciliano²,

Staropoli³

et al. 2022

Molecules

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Rare-earth elements (REEs) are in all respect a class of new contaminants that may have toxic effects on organisms and microorganisms and information on their interactions with natural ligands should be of value to predict and control their diffusion in natural environments. In the current study, we investigate interactions of tripositive cations of praseodymium, europium, holmium, and thulium with harzianic acid (H2L), a secondary metabolite produced by selected strains of fungi belonging to the Trichoderma genus. We applied the same techniques and workflow previously employed in an analogous study concerning lanthanum, neodymium, samarium, and gadolinium tripositive cations. Therefore, in the current study, HPLC–ESI–HRMS experiments, circular dichroism (CD), and UV-Vis spectrophotometric absorption data, as well as accurate pH measurements, were applied to characterize bonding interactions between harzianic acid and Pr3+, Eu3+, Ho3+, and Tm3+ cations. Problems connected to the low solubility of harzianic acid in water were overcome by employing a 0.1 M NaClO4/(CH3OH + H2O 50/50 w/w) mixed solvent. For Pr3+, Ho3+, and Tm3+, only the mono complexes PrL+, HoL+, and TmL+ were detected and their formation constant determined. Eu3+ forms almost exclusively the bis complex EuL2− for which the corresponding formation constant is reported; under our experimental conditions, the mono complex EuL+ is irrelevant. Combining the results of the present and previous studies, a picture of interactions of harzianic acid with rare-earth cations extending over 8 of the 17 REEs can be composed. In order to complement chemical information with toxicological information, a battery of bioassays was applied to evaluate the effects of praseodymium, europium, holmium, and thulium tripositive cations on a suite of bioindicators including Aliivibrio fischeri (Gram-negative bacterium), Raphidocelis subcapitata (green alga), and Daphnia magna (microcrustacean), and median effective concentration (EC50) values of Pr3+, Eu3+, Ho3+, and Tm3+ for the tested species were assessed.

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Rare earth elements in flour samples. A potential transfer from cricket food to the human level through the trophic chain

Ruffolo,

Brouziotis,

Di Natale

et al. 2024

Journal of Food Composition and Analysis

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A first attempt to evaluate the toxicity to Phaeodactylum tricornutum Bohlin exposed to rare earth elements

Cited by 5 publications

References 45 publications

Multi-Endpoint Analysis of Cerium and Gadolinium Effects after Long-Term Exposure to Phaeodactylum tricornutum

Multi-Endpoint Analysis of Cerium and Gadolinium Effects after Long-Term Exposure to Phaeodactylum tricornutum

Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr3+, Eu3+, Ho3+, Tm3+)

Rare earth elements in flour samples. A potential transfer from cricket food to the human level through the trophic chain

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