A Fine Structure Study of Injury to the Endothelial Cells of the Rabbit Abdominal Aorta by Various Stimuli

Hoff, Hans; Gottlob, R

doi:10.1177/000331976701800704

Cited by 37 publications

(3 citation statements)

References 34 publications

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“…Such giant vacuoles have been described in mammalian endothelium by several investigators and are usually interpreted as representing a reaction to cell injury such as mechanical trauma, hypoxia, freezing, exposure to hyperosmotic solutions, etc. (Luse and Harris 1960, Hoff and Gottlob 1967, Ts'ao and Spaet 1967. Different theories have been proposed for the genesis of these vacuoles: Mitochondria] swelling, autolysis of cytoplasmic content or dilatation of endoplasmatic reticulum.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Studies of the Capillary Structure in the rete mirabile of the Eel, Anguilla vulgaris L.

Stray‐Pedersen

Nicolaysen

1975

Acta Physiologica Scandinavica

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The capillaries of the counter-current capillary organ, rete mirabile, have been studied by light microscopy and electron microscopy. The vasculature of this rete was found to have a cross-sectional area of about 5.25 mm2, and it consisted of 34,000 efferent (=arterial) and 22,000 afferent (=venous) capillaries. The total surface area was the same for the two types of capillaries. The capillary endothelial cells showed numerous pinocytotic vesicles, scattered giant vacuoles and cytoplasmic extrusions (microvilli) at the luminal surface. The majority of the intercellular junctions appeared to have patent gaps with a width of 110-120 A. The arterial capillaries appeared in many ways to be morphologically similar to skeletal muscle capillaries of mammals, whereas the fenestrated venous capillaries resembled those in the intestinal mucosa of mammals. Measurements of the ionic composition of the rete tissue indicated that the endothelial cells contained much less K+ than other cells, the rete containing approximately equimolar amounts of K+ and Na+. The functional significance of the structural and chemical observations are discussed.

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Section: Discussionmentioning

confidence: 99%

Quantitative Studies of the Capillary Structure in the rete mirabile of the Eel, Anguilla vulgaris L.

Stray‐Pedersen

Nicolaysen

1975

Acta Physiologica Scandinavica

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“…Shimamoto [13] found that the edematous arterial reaction was induced by single injection of adrenaline or drip infusion of brady kinin and H off et al [6] reconfirmed the edematous arterial reaction by adrenaline. They also reported that this reaction was produced by the first adrenaline injection and failed to produce by the second injection given successively, namely adrenaline exhibited a tachy phylaxis in producing the edematous arterial reaction, while the edematous arterial reaction has been presumed to be originated or enhanced by the venoconstriction in the vasa vasorum in arterial wall.…”

Section: Discussionmentioning

confidence: 98%

The Venoconstrictive Property of Adrenaline and Bradykinin and their Tachyphylaxis in Isolated Rabbit Saphenous Veins

Ishioka¹,

Matsumura²,

Honda³

et al. 1969

J Vasc Res

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“…Such pale cells were also found just basal to the intact endothelial lining. 3. Endothelial cells often sent pseudopodia into the lumen, either as small foot processes or as large bulges containing the nuclei.…”

mentioning

confidence: 99%

Regeneration in Large Blood Vessels Following Mild Mechanical Trauma

Hoff

Gottlob²

1969

Angiology

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Earlier investigations by Gottlob and Zinnerl showed distinct differences in the morphologically characterized changes between hard and mild mechanical trauma on blood vessels with light microscopy of silver-stained surface preparations. Whereas hard mechanical trauma, induced by ligation of the vessel wall with thick silk thread, produced complete endothelial cell destruction at the ligated area and eventual overgrowth by regenerating endothelial cells, mild mechanical trauma induced by compressing the vessel wall with a clamp covered by rubber tubing was characterized by early focal endothelial injury, eventual formation of multinucleated endothelial cells and endothelial phagocytosis of blood cell degradation products. Although hard mechanical trauma has been used by a number of investigators as a model for studies on thrombosis and endothelial regeneration, it is felt that mild mechanical trauma mimics more closely pathophysiologic phenomena in human circulation, such as inflammation, aging and surgical injury. Furthermore, recent investigations by Friedman and Byers2 have indicated that mechanical injury to the intimal lining of the arterial wall enhances atherosclerosis in cholesterol-fed rabbits. It would be of interest to see if any of the morphologic changes characteristic of very early atherosclerosis could be observed at periods following mild mechanical trauma even without inducing hyperlipemia. An investigation with the electron microscope was undertaken to study morphologic alterations in the wall of the rabbit abdominal aorta and inferior vena cava at periods ranging from one to seven days following application of mild mechanical trauma. It was felt that under the high resolution of the electron microscope, one would obtain additional information to the light microscopic studies on mild mechanical trauma previously performed. A rubber-coated bulldog clamp was applied concurrently to the rabbit abdominal aorta and inferior vena cava at the level of the renal bifurcation for 10 sec. At periods of one, two, three, five and seven days after inducing injury, the blood vessels were fixed with glutaraldehyde by an in situ perfusion technique, then excised and carried through the standard electron microscopy procedure.In contrast to what one would have expected, no progression of changes in the ultrastructural morphology of the blood vessels could be found between one and seven days after trauma. The following changes were observed at all times investigated both in the aorta and vena cava.

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A Fine Structure Study of Injury to the Endothelial Cells of the Rabbit Abdominal Aorta by Various Stimuli

Cited by 37 publications

References 34 publications

Quantitative Studies of the Capillary Structure in the rete mirabile of the Eel, Anguilla vulgaris L.

Quantitative Studies of the Capillary Structure in the rete mirabile of the Eel, Anguilla vulgaris L.

The Venoconstrictive Property of Adrenaline and Bradykinin and their Tachyphylaxis in Isolated Rabbit Saphenous Veins

Regeneration in Large Blood Vessels Following Mild Mechanical Trauma

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