A field-applicable colorimetric assay for notorious explosive triacetone triperoxide through nanozyme-catalyzed irreversible oxidation of 3, 3′-diaminobenzidine

Jangi, Saeed Reza Hormozi; Akhond, Morteza; Absalan, Ghodratollah

doi:10.1007/s00604-020-04409-1

Cited by 49 publications

(41 citation statements)

References 56 publications

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“…While many modifications of substrates to enhance the signal have been reported for enzymes, only a few papers discuss these modifications for nanozymes. 24–26 Finally, the selection of the substrate composition should also consider the stability of the components. While for some nanozymes, high concentrations of substrates may be favourable, the applicability of such substrate solutions will be limited by the high background caused by the self-oxidation of substrates.…”

Section: Discussionmentioning

confidence: 99%

Characterization of nanozyme kinetics for highly sensitive detection

Panferov,

Wang,

Liu

2024

Analyst

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Section: Discussionmentioning

confidence: 99%

Characterization of nanozyme kinetics for highly sensitive detection

Panferov,

Wang,

Liu

2024

Analyst

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“…The results are shown in Figure 2, as shown in this figure, in the presence of TMB, the as-synthesized nanozymes catalyze the oxidation process of TMB by hydrogen peroxide to produce its corresponding bluecolored cation radical, TMB-ox with a shoulder 440-485 nm (λmax of 460 nm) and a symmetric spectrum over 500-750 nm (λmax of 658 nm). In fact, during the oxidation of TMB silver nanozymes produce active hydroxyl radicals by acting on hydrogen peroxide [2,8,[10][11][12]. The produced radicals then oxidize the TMB molecules to their corresponding cation radicals via a 2-electron reversible oxidation pathway.…”

Section: Evaluating Peroxidase-like Activity Of As-prepared Nanozymesmentioning

confidence: 99%

Evaluating the Effect of Shelf-Storage, Daylight, and Air Oxygen on the Peroxidase-like Activity of Unmodified Silver Nanoparticles

Jangi¹

2023

Preprint

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In this contribution, unmodified silver nanoparticles were synthesized and characterized for their size and morphological properties. Thereafter, their peroxidase-like activity as the common catalytic property of silver nanoparticles was investigated by catalyzing the oxidation of 3,3’,5,5’-tetramethyl-benzidine (TMB) as peroxidase substrate, exhibiting, a specific activity as high as 5.4 µM min-1 for the as-prepared unmodified silver nanoparticles. The stability of the catalytic activity of the as-prepared nanozymes was also checked upon their storage at ambient temperature within 7 days at different storage conditions. The results revealed that the peroxidase-like activity of unmodified silver nanoparticles was approximately retained at about 75%, and 63% after 7 days exposing daylight and air oxygen, in order. The shelf-life (storage stability) of the as-prepared nanozymes was also investigated at usual storage conditions (i.e., 4 ℃ under dark), revealed that the nanozymes saved their activity about 96% of their initial activity after 10 days of storage at 4 ℃ under dark conditions.

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“…The peak at 536 nm corresponds to intramolecular transition of benzenoid rings into quinoid rings 16,17 . The HRP(+)/PDAB spectra also exhibits an absorption peak at 370 nm, and the 460 nm peak corresponds to the characteristic absorption of PDAB 18 . Finally, confocal Raman microscopy was used to examine single cells with and without PANI (Fig.…”

Section: Spectroscopic Characterizations Of In Situ Deposited Polymersmentioning

confidence: 99%

In situ genetically targeted chemical assembly of polymers on living neuronal membranes

Zhang

Loh

Kadur

et al. 2022

Preprint

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Multicellular biological systems, most notably living neural networks, exhibit highly complex physical organization properties that pose challenges for building cell-specific and biocompatible interfaces. We developed a novel approach to genetically program cells to chemically assemble artificial structures that modify the electrical properties of neurons in situ, opening up the possibility of minimally-invasive cell-specific interfaces with neural circuits in living animals. However, the efficiency and biocompatibility of this approach were challenged by limited membrane targeting of the constructed material. Here, we report a method with significantly improved molecular construct properties, which expresses highly localized enzymes targeted to the plasma membrane of primary neurons with minimal intracellular retention. Polymers synthesized in situ by this approach form dense clusters on the targeted cell membrane, and neurons remain viable after polymerization. This platform can be readily extended to incorporate a broad range of materials onto the surface membranes of specific cells within complex tissues, using chemistry that may further enable the next generation of interfaces with living biological systems.

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A field-applicable colorimetric assay for notorious explosive triacetone triperoxide through nanozyme-catalyzed irreversible oxidation of 3, 3′-diaminobenzidine

Cited by 49 publications

References 56 publications

Characterization of nanozyme kinetics for highly sensitive detection

Characterization of nanozyme kinetics for highly sensitive detection

Evaluating the Effect of Shelf-Storage, Daylight, and Air Oxygen on the Peroxidase-like Activity of Unmodified Silver Nanoparticles

In situ genetically targeted chemical assembly of polymers on living neuronal membranes

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