A fibrous bed bioreactor to improve the productivity of succinic acid by Actinobacillus succinogenes

Abstract

BACKGROUND Succinic acid is one of the most important biorefinery platform chemicals. The fibrous‐bed bioreactor (FBB), comprising spiral cotton fiber packed in a modified bioreactor, is effective for anaerobic cell‐immobilized fermentation. In this study, fermentation production of succinic acid by Actinobacillus succinogenes using a FBB was investigated. RESULTS The effect of different initial glucose concentrations on succinic acid production in batch fermentation in a FBB was examined, which indicated that the optimal initial glucose concentration was 55 g L‐1. In 10 batches of repeated‐batch fermentation, the average succinic acid productivity, concentration, and yield were 3.61 g L‐1 h‐1, 39.0 g L‐1, and 0.85 g g‐1, respectively. A maximum succinic acid concentration of 98.7 g L‐1 was reached in fed‐batch fermentation comprising four batches of fermentation, with a succinic acid yield of 0.89 g g‐1 and productivity of 2.77 g L‐1 h‐1. CONCLUSION Succinic acid fermentation by A. succinogenes in a FBB was successfully performed to enhance succinic acid productivity. And also, high succinic acid concentration with high reactor productivity and yield were simultaneously achieved in the FBB with fed‐batch fermentation. © 2013 Society of Chemical Industry

Process design and economic evaluation of integrated, multi‐product biorefineries for the co‐production of bio‐energy, succinic acid, and polyhydroxybutyrate (PHB) from sugarcane bagasse and trash lignocelluloses

“…To use the monomeric sugars present in pretreated sugarcane bagasse and trash, such as pentose, arabinose and cellobiose, more efficiently, the PHB bioproduct yield could be increased through additional genetic engineering and production development (i.e. fermentation) of recombinant E. coli, similarly to what has been done to the SA‐producing micro‐organism A. succinogenes …”

“…An SA seed train provided inoculum to minimize the micro‐organism's lag phase and promote exponential growth using the typical size of 10% (v/v) of the fermentation volume . Ninety percent of the feed stream was therefore pumped to the fermentation area and preheated prior to fermentation at 38°C . The nutrient medium, which was used in the seed train and fermentation area, was sterilized at 120°C for 15 min.…”

“…For a glucose‐only sugar stream, an initial sugar concentration of ≤100 g L −1 is typically required for A. succinogenes, and sugar concentrations of 50–80 g L −1 have been obtained and used for pretreated lignocelluloses reported previously . For Scenario C a fed‐batch fermentation strategy was used whereby a portion of the sugar feed stream was sent to a triple‐effect evaporator to concentrate the combined glucose rich and hemicellulose hydrolysate stream to 200 g L −1 xylose and glucose …”

Efficient production of poly‐γ‐glutamic acid from cane molasses by Bacillus subtilisNX‐2 immobilized on chemically modified sugarcane bagasse

“…Sugarcane bagasse, a lignocellulose material, is the major by‐product of the sugar cane industry . The bagasse surface is arranged in a crisscross pattern by the fiber matrix, which can increase the surface area to support cell attachment.…”

Enhanced succinic acid productivity by expression of mgtCB gene in Escherichia coli mutant

“…In our previous study, a succinic acid production of 129.21 g L −1 with a productivity of 2.15 g L −1 h −1 was achieved under a same fermentation condition via the overexpression of mgtA in KMG111 to increase the concentration of intracellular Mg 2+ . Ma et al [15] obtained a production of 79.0 g L −1 succinic acid by using a nonrecombinant E. coli strain (AFP11), but their overall productivity of 1.05 g L −1 h −1 is relatively lower, It was also reported that A. succinogenes could produce 39.0 g L −1 succinic acid with a productivity of 3.61 g L −1 h −1 and a yield of 0.85 g g −1 glucose [34] and C. glutamicum is able to produce 146 g L −1 succinic acid in 46 h with a productivity of 3.17 g L −1 h −1 [22]. But, the high productivity was achieved under the high cost fermentation media.…”