A fibrosarcoma model derived from mouse embryo cells: Growth properties and secretion of collagenase and metalloproteinase inhibitor (TIMP) by tumour cell lines

Hicks, Nigel John; Ward, Robin V.; Reynolds, John J.

doi:10.1002/ijc.2910330620

Cited by 67 publications

(16 citation statements)

References 39 publications

(35 reference statements)

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“…Lohmander et al also found that the MMP-3 concentration in the synovial fluid of patients with osteoarthritis of the knee increases as the disease progresses, suggesting that MMP-3 is an index of cartilage destruction [11]. TIMP, on the other hand, acts as a regulator of MMP activity and as a protective factor against metastasis and invasion of malignant neoplasms [2,10]. In cartilage, TIMP-1 not only prevents matrix destruction as an MMP-inhibiting protein but also contributes to matrix maintenance by promoting cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Changes in biochemical parameters after anterior cruciate ligament injury

Handa

Shirakura

Kimura³

et al. 2005

International Orthopaedics (SICO

106

133

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We studied the biochemical characteristics of human knees with deficient anterior cruciate ligaments (ACL) and analysed their relationship to the time after ligamentous injury. Thirty-two patients with isolated ACLinjured knees and six healthy volunteers were enrolled. Synovial fluid samples were centrifuged after aspiration during arthroscopic examination, and aliquots of supernatant were frozen and stored at −80°C. The samples were analysed for interleukin (IL)-1β, tumour necrosis factor (TNF)-α, IL-6, matrix metalloproteinase (MMP)-3, and tissue inhibitor of metalloproteinase (TIMP)-1 using commercially available sandwich enzyme-linked immunosorbent assay. In fluid from ACL-injured knees, the average concentrations of IL-6, MMP-3 and TIMP-1 were highly elevated in comparison with normal controls. There was a statistically significant correlation between the concentrations of MMP-3 and IL-6. The IL-6 and TIMP-1 concentrations were interrelated. The concentration of MMP-3 remained high, independent of the duration since the injury, whereas the TIMP-1 and IL-6 levels decreased. The results suggest that the timing of the treatment of an ACL-injured knee might be of importance.Résumé Nous avons étudié les caractéristiques biochimiques des genoux humains avec un ligament croisé antérieur défectueux (LCA) et avons analysé leur rapport au temps après la lésion ligamentaire. Trente-deux malades avec une lésion isolée du LCA et six volontaires sains ont été enrôlés.

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Section: Discussionmentioning

confidence: 99%

Changes in biochemical parameters after anterior cruciate ligament injury

Handa

Shirakura

Kimura³

et al. 2005

International Orthopaedics (SICO

106

133

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“…sion. Furthermore, the consistent expression of An inverse correlation between TIMP-1 levels and metaTIMPs in many cell types of the noncancerous liver static ability has been demonstrated in a series of fibrosarcosuggests some unknown functional role that must be mas, 7 of local tissue invasion. 12 TIMP-2 also blocks invasion of fibro- Received December 11, 1995; accepted March 15, 1996. sarcoma cells to recombinant basement membrane.…”

Section: Homogeneous Expression Of Timps In Cancer Cellsmentioning

confidence: 92%

“…sion. Furthermore, the consistent expression of An inverse correlation between TIMP-1 levels and metaTIMPs in many cell types of the noncancerous liver static ability has been demonstrated in a series of fibrosarcosuggests some unknown functional role that must be mas, 7 and the inhibitory effects of TIMPs on tumor invasion clarified. (HEPATOLOGY 1996;24:82-88.…”

Section: Homogeneous Expression Of Timps In Cancer Cellsmentioning

confidence: 95%

Cellular Distribution of Transcripts for Tissue Inhibitor of Metalloproteinases 1 and 2 in Human Hepatocellular Carcinomas

et al. 1996

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HARUSHIGE NAKATSUKASA, KOUZOU ASHIDA, TOSHIHIRO HIGASHI, SOUHEI OHGUCHI, SO TSUBOI, NAOKI HINO, KAZUHIRO NOUSO, YOSHIAKI URABE, NOBUYUKI KINUGASA, KEIGO YOSHIDA, SHUJI UEMATSU, MASAHIKO ISHIZAKI, YOSHIYUKI KOBAYASHI, AND TAKAO TSUJI Hepatocellular carcinoma (HCC) is one of the most prevaThe cellular distribution of tissue inhibitor of melent malignancies in Japan and China, and frequently occurs talloproteinases (TIMP)-1, and TIMP-2 was studied by in hepatitis B or C virus-related chronic hepatitis or cirrhousing in situ hybridization in surgically removed husis. In particular, cirrhosis has been regarded as a high-risk man hepatocellular carcinomas (HCCs) and cholandisease state for developing HCCs. A unique feature of HCC giocellular carcinomas (CCCs). The purpose of this development is the occurrence of the tumor in fibrotic or cirstudy was to characterize the potential involvement rhotic liver that contains abundant extracellular matrices of TIMPs in the development of HCCs and CCCs. All (ECMs). Therefore, the capacity of HCC to degrade the surHCCs and CCCs expressed TIMPs. The distribution rounding ECMs may be an important trait for growth, invaof transcripts for TIMPs in the tumors was mostly sion, and metastasis. homogeneous. Expression of TIMPs in cancer cellsThe characteristic features of malignant tumors are the was more intense than that in the surrounding noninvasion to cross tissue boundaries and the metastasis to cancerous liver (either, cirrhosis, chronic hepatitis, distant organs. Many steps that occur during cancer invasion or normal), and expression of TIMP-1 was stronger and metastasis require specific interactions between maligthan that of TIMP-2. Expression of TIMPs varied nant cells and the ECMs, 1 particularly in regard to HCC and among HCC nodules, but there was no obvious associcirrhotic liver. Multiple humoral factors are involved in this ation between the expression level of TIMPs and difprocess: matrix metalloproteinases (MMPs), tissue inhibitor ferentiation stages or invasiveness of the HCCs. Tranof metalloproteinases (TIMPs), and various cytokines. An imscripts for TIMPs were clearly demonstrated in the balance between TIMPs and MMPs may be an important metastatic HCC nodules in the lung. Expression of factor in tumor invasion and metastasis. TIMP-1 in CCC was strong, and small nodules of CCC Three members of the TIMP family have been described. 2 were recognized in the liver. ImmunohistochemicalEach TIMP is the product of a separate gene. TIMP-1 is a 28-study for TIMP-1 revealed a consistent staining of the kd glycoprotein, whereas TIMP-2 is a 21-kd nonglycosylated TIMP-1 protein with the transcripts. In the perituprotein. Between TIMP-1 and TIMP-2, there is 37% amino moral histologically normal liver, which was not inacid identity and 65.6% overall homology. 3 TIMP-3 has been fected with either hepatitis B or C virus, expression identified as a 21-kd protein and shares an amino acid seof TIMP-1 was found in various cell types, but that of quence homology of 40% with TIMP-1 and 45%...

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“…Expression of TIMP mRNA or protein has not yet been examined in the DMBA/TPA model of skin carcinogenesis, but decreased levels of TIMP has been correlated with tumor invasion and metastasis in several systems [81,82]. It is interesting that the antisense-TIMP cell lines that expressed dramatic differences in their metastatic potential in nude mice had only approximately a 50% decrease in the amount of TIMP protein expressed as compared to the parental control cells [52].…”

Section: Parallels Between Expression Of Stromelysin and Current Concmentioning

confidence: 99%

Stromelysin in tumor progression and metastasis

1990

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There are several characteristics of stromelysin that suggest that expression of this enzyme may play an important role in tumor invasion and metastasis; the stromelysin gene is expressed in response to stimulation by oncogenes and tumor promoters, and the protein product of this gene is a metalloproteinase capable of degrading multiple components of the extracellular matrix. Experimental evidence to support this hypothesis has been derived from several animal model systems, in which a positive correlation has been observed between stromelysin expression and tumor progression and metastasis. In addition, in vivo experiments in which the levels of TIMP, the tissue inhibitor of metalloproteinases, were altered also strongly suggest a causal role for metalloproteinases in tumor metastases. The expression of active stromelysin in tumor cells requires the fulfillment of several criteria, and this multistep process is reminiscent of the molecular events that are currently understood to contribute to tumor progression and carcinogenesis. Expression of stromelysin mRNA requires both a stimulus, a step which may correspond to the activation of an oncogene in multistep carcinogenesis, as well as the lifting of transcriptional repression, which may correspond to the loss of tumor suppressor function. Both positive and negative modulation of stromelysin transcription appear to utilize pathways that involve the protooncogenes c-fos and/or c-jun. The expression of active stromelysin enzyme also requires conversion of the proenzyme to an active form, and a proper balance between the expression of inhibitors and the levels of active enzyme. The multiple levels of stromelysin regulation support the concept of multistep carcinogenesis and may provide a tool for further understanding of the molecular nature of the events that lead to tumor progression, invasion, and metastasis.

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A fibrosarcoma model derived from mouse embryo cells: Growth properties and secretion of collagenase and metalloproteinase inhibitor (TIMP) by tumour cell lines

Cited by 67 publications

References 39 publications

Changes in biochemical parameters after anterior cruciate ligament injury

Changes in biochemical parameters after anterior cruciate ligament injury

Cellular Distribution of Transcripts for Tissue Inhibitor of Metalloproteinases 1 and 2 in Human Hepatocellular Carcinomas

Stromelysin in tumor progression and metastasis

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