A fibronectin-related synthetic peptide, ProAlaSerSer, inhibits fibronectin binding to the cell surface, fibronectin-promoted cell migration in vitro, and cell migration in vivo

Katow, Hideki; Yazawa, Sadaharu; Sofuku, Shosuke

doi:10.1016/0014-4827(90)90138-z

Cited by 28 publications

(14 citation statements)

References 20 publications

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“…The migration involves an RGD amino acid sequence-mediated cell-substrate interaction, 80,81 which was initially found in the ECM and fibronectin, as a cell adhesion active motif. 82 Fibronectin was detected on the surface of PMCs, and the RGD motif was found in the sea urchin fibronectin gene, 8 suggesting that the ECM protein receptor is present on the basal surface of the ectoderm.…”

Section: Integrinsmentioning

confidence: 99%

Mechanisms of the epithelial-to-mesenchymal transition in sea urchin embryos

Katow

2015

Tissue Barriers

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Section: Integrinsmentioning

confidence: 99%

Mechanisms of the epithelial-to-mesenchymal transition in sea urchin embryos

Katow

2015

Tissue Barriers

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“…Fibronectin is involved in adhesion between tissues and cells or between cells (13). It can induce cell migration associated with the reconstruction of an organism in the process of wound healing (14). Matrix metalloproteinases (MMPs) play an important role in controlling extracellular matrix contents in the damaged organism.…”

Section: Introductionmentioning

confidence: 99%

Skin Wound Healing Effects and Action Mechanism of Acai Berry Water Extracts

2017

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The purpose of this study was to investigate the wound healing effect of acai berry water extracts (ABWE) and a possible underlying mechanism involved in its action using various in vitro and in vivo models. The wound healing effect of ABWE was evaluated by migration assay using HS68 fibroblast cells. In addition, its effect on mRNA expression of procollagen, fibronectin, and MMP-1 was determined. Moreover, the wound healing effect of ABWE was evaluated in in vivo wound models through macroscopic and microscopic observation. In addition, mRNA expression levels of wound related genes were determined. Results revealed that ABWE was not cytotoxic. It increased migration of HS68 fibroblast cells. ABWE increased mRNA expression levels of fibronectin but decreased the mRNA expression levels of MMP-1. ABWE also showed significantly potent wound healing effect in vivo based on macroscopic and histopathological observation and mRNA expression evaluation for wound related genes. Taken together, our results indicated that ABWE might have potential as a wound healing agent.

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“…Katow 1987Katow , 1990Katow et al 1990Katow et al , 1991Katow et al , 1997. Katow 1987Katow , 1990Katow et al 1990Katow et al , 1991Katow et al , 1997.…”

Section: Discussionunclassified

“…In echinoderm embryos, two synthetic peptides of fibronectin, Arg-Gly-Asp-Ser (RGDS) and Pro-Ala-Ser-Ser (PASS), significantly interfere with morphogenetic movements such as primary mesenchyme cell (PMC) migration and gastrulation by disturbing cell-ECM binding (Katow 1987(Katow , 1990Katow et al 1990Katow et al , 1991. Similarly disturbed morphogenesis occurs by introducing antihuman fibronectin antibodies to the blastocoele (Katow 1990).…”

Section: Introductionmentioning

confidence: 99%

An RGDS peptide‐binding receptor, FR‐1R, localizes to the basal side of the ectoderm and to primary mesenchyme cells in sand dollar embryos

Katow¹,

Sofuku

2001

Dev Growth Differ

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Immunoblotting using polyclonal antibodies (pAb) raised against an FR-1 receptor (FR-1R), a 57 kDa Arg-Gly-Asp-Ser (RGDS)-binding protein, of the sand dollar Clypeaster japonicus showed that the pAb monospecifically bound to the protein. FR-1R was present in purified plasma membrane, suggesting that the protein is a membrane-bound protein. The molecular structure of FR-1R did not change throughout the early embryogenesis, whereas its expression changed significantly during this period. FR-1R was present in the cortex of unfertilized eggs and was then transferred to the hyaline layer soon after the fertilization. The hyaline layer retained FR-1R immunoreactivity during early embryogenesis. FR-1R appeared on the basal side of the ectoderm at the morula stage and was retained basolaterally, at least, to the early gastrula stage. In mesenchyme blastulae, FR-1R was also present on the surface of primary mesenchyme cells (PMC). FR-1R was localized on the basal side of the ectoderm in early gastrulae, exclusively at the place where PMC formed ventrolateral aggregates, and at the apical tuft ectoderm. In vitro, PMC bound to FR-1R and its binding was inhibited in the presence of a synthetic RGDS peptide or the pAb. The pAb introduced into the blastocoele perturbed PMC migration and gastrulation. FR-1R was weakly recognized by antihuman integrin beta5 subunit pAb.

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A fibronectin-related synthetic peptide, ProAlaSerSer, inhibits fibronectin binding to the cell surface, fibronectin-promoted cell migration in vitro, and cell migration in vivo

Cited by 28 publications

References 20 publications

Mechanisms of the epithelial-to-mesenchymal transition in sea urchin embryos

Mechanisms of the epithelial-to-mesenchymal transition in sea urchin embryos

Skin Wound Healing Effects and Action Mechanism of Acai Berry Water Extracts

An RGDS peptide‐binding receptor, FR‐1R, localizes to the basal side of the ectoderm and to primary mesenchyme cells in sand dollar embryos

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