A ferritin-like protein with antioxidant activity in Ureaplasma urealyticum

Dai, Guozhi; Li, Ranhui; Chen, Hongliang; Jiang, Chao; You, Xiaoxing; Wu, Yimou

doi:10.1186/s12866-015-0485-6

Cited by 10 publications

(5 citation statements)

References 50 publications

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“…Free Fe can interfere with the manganese complexes that are used by L. plantarum’s catalase to lower reactive oxygen levels [78]. Thus, the DNA-binding ferritin-like protein could help L. plantarum tolerate the hydrogen peroxide by binding to and storing excess free Fe that would otherwise damage the cell [79]. The second coding sequence encodes a protein that has homology with a low temperature requirement C protein of Listeria monocytogenes , which may be involved in lipid metabolism [80].…”

Section: Resultsmentioning

confidence: 99%

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus plantarum—A Novel Lineage of Lactobacillus Phages Comprising Five New Members

Kyrkou

Carstens

Ellegaard-Jensen

et al. 2019

Viruses

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Lactobacillus plantarum is a bacterium with probiotic properties and promising applications in the food industry and agriculture. So far, bacteriophages of this bacterium have been moderately addressed. We examined the diversity of five new L. plantarum phages via whole genome shotgun sequencing and in silico protein predictions. Moreover, we looked into their phylogeny and their potential genomic similarities to other complete phage genome records through extensive nucleotide and protein comparisons. These analyses revealed a high degree of similarity among the five phages, which extended to the vast majority of predicted virion-associated proteins. Based on these, we selected one of the phages as a representative and performed transmission electron microscopy and structural protein sequencing tests. Overall, the results suggested that the five phages belong to the family Myoviridae, they have a long genome of 137,973–141,344 bp, a G/C content of 36.3–36.6% that is quite distinct from their host’s, and surprisingly, 7 to 15 tRNAs. Only an average 41/174 of their predicted genes were assigned a function. The comparative analyses unraveled considerable genetic diversity for the five L. plantarum phages in this study. Hence, the new genus “Semelevirus” was proposed, comprising exclusively of the five phages. This novel lineage of Lactobacillus phages provides further insight into the genetic heterogeneity of phages infecting Lactobacillus sp. The five new Lactobacillus phages have potential value for the development of more robust starters through, for example, the selection of mutants insensitive to phage infections. The five phages could also form part of phage cocktails, which producers would apply in different stages of L. plantarum fermentations in order to create a range of organoleptic outputs.

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Section: Resultsmentioning

confidence: 99%

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus plantarum—A Novel Lineage of Lactobacillus Phages Comprising Five New Members

Kyrkou

Carstens

Ellegaard-Jensen

et al. 2019

Viruses

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“…Furthermore, similar findings demonstrated that M. pneumoniae and M. genitalium encode a homologous protein known as peptide methionine sulfoxide reductase (MsrA), which is an antioxidant repair enzyme that catalyzes the reduction of methionine sulfoxide [Met(O)] residues in proteins to methionine (Dhandayuthapani et al, 2001). In addition, in 2015, our group found that a ferritin-like protein with ferroxidase activity in U. urealyticum could impair harmful oxidative production in vitro (Dai et al, 2015). On the basis of these known data, the mechanism employed by mycoplasma in reaction to oxidative stress plays an important role in mycoplasma survival within host.…”

Section: Overcoming the Immune Effector Molecules Assaultsmentioning

confidence: 99%

Subversion of the Immune Response by Human Pathogenic Mycoplasmas

2019

Self Cite

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Mycoplasmas are a large group of prokaryotes which is believed to be originated from Gram-positive bacteria via degenerative evolution, and mainly capable of causing a wide range of human and animal infections. Although innate immunity and adaptive immunity play crucial roles in preventing mycoplasma infection, immune response that develops after infection fails to completely eliminate this bacterium under certain circumstances. Thus, it is reasonable to speculate that mycoplasmas employ some mechanisms to deal with coercion of host defense system. In this review, we will highlight and provide a comprehensive overview of immune evasion strategies that have emerged in mycoplasma infection, which can be divided into four aspects: (i) Molecular mimicry and antigenic variation on the surface of the bacteria to evade the immune surveillance; (ii) Overcoming the immune effector molecules assaults: Induction of detoxified enzymes to degradation of reactive oxygen species; Expression of nucleases to degrade the neutrophil extracellular traps to avoid killing by Neutrophil; Capture and cleavage of immunoglobulins to evade humoral immune response; (iii) Persistent survival: Invading into the host cell to escape the immune damage; Formation of a biofilm to establish a persistent infection; (iv) Modulation of the immune system to down-regulate the intensity of immune response. All of these features increase the probability of mycoplasma survival in the host and lead to a persistent, chronic infections. A profound understanding on the mycoplasma to subvert the immune system will help us to better understand why mycoplasma is so difficult to eradicate and ultimately provide new insights on the development of therapeutic regimens against this bacterium in future.

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“…Free Fe can interfere with the manganese complexes that are used by L. plantarum's catalase to lower reactive oxygen levels [80]. Thus, the DNA-binding ferritin-like protein could help L. plantarum tolerate the hydrogen peroxide by binding to and storing excess free Fe that would otherwise damage the cell [81]. The second coding sequence encodes a protein that has homology with a low temperature requirement C protein of Listeria monocytogenes, which may be involved in lipid metabolism [82].…”

Section: Other Predicted Proteinsmentioning

confidence: 99%

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus Plantarum - A Novel Lineage of Lactobacillus Phages Comprising Five New Members

Kyrkou

Carstens

Ellegaard-Jensen

et al. 2019

Preprint

View full text Add to dashboard Cite

Lactobacillus plantarum is a bacterium with promising applications to the food industry and agriculture and probiotic properties. So far, bacteriophages of this bacterium have been moderately addressed. We examined the diversity of five new L. plantarum phages via whole genome shotgun sequencing and in silico protein predictions. Moreover, we looked into their phylogeny and their potential genomic similarities to other complete phage genome records through extensive nucleotide and protein comparisons. These analyses revealed a high degree of similarity among the five phages, which extended to the vast majority of predicted virion-associated proteins. Based on these, we selected one of the phages as a representative and performed transmission electron microscopy and structural protein sequencing tests. Overall, the results suggested that the five phages belong to the family Myoviridae, they have a long genome of 137.973-141.344 bp, a G/C content of 36,3-36,6% that is quite distinct from their host’s, and, surprisingly, seven to 15 tRNAs. Only an average 41/174 of their predicted genes were assigned a function. The comparative analyses unraveled considerable genetic diversity for the five L. plantarum phages of this study. Hence, the new genus “Semelevirus” was proposed, which comprises exclusively the five phages. This novel lineage of Lactobacillus phages provides further insight into the genetic heterogeneity of phages infecting Lactobacillus sp.. The five new Lactobacillus phages have a potential value for the development of more robust starters through, for example, the selection of mutants insensitive to phage infections. The five phages could also form part of phage cocktails, which producers would apply in different stages of L. plantarum fermentations in order to create a range of organoleptic outputs.

show abstract

A ferritin-like protein with antioxidant activity in Ureaplasma urealyticum

Cited by 10 publications

References 50 publications

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus plantarum—A Novel Lineage of Lactobacillus Phages Comprising Five New Members

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus plantarum—A Novel Lineage of Lactobacillus Phages Comprising Five New Members

Subversion of the Immune Response by Human Pathogenic Mycoplasmas

Expanding the Diversity of Myoviridae Phages Infecting Lactobacillus Plantarum - A Novel Lineage of Lactobacillus Phages Comprising Five New Members

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