A Ferret Model of Encephalopathy of Prematurity

Wood, Thomas H.; Moralejo, Daniel H.; Corry, Kylie A.; Snyder, Jessica M.; Traudt, Christopher M.; Curtis, Chad; Nance, Elizabeth; Parikh, Pratik; Juul, Sandra E.

doi:10.1159/000498968

Cited by 9 publications

(14 citation statements)

References 37 publications

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“…Another limitation of AGA is the maximum size of the animals eligible for testing due to the limited dimensions of the apparatus. Therefore, the use of AGA is currently limited to animals the size of rodents and ferrets 45 . Additionally, recently emerging neurobehavioral assessment approaches in the field of motion tracking capable of machine-learning may surpass AGA in both comprehensiveness as well as possible applications 18 , 19 , 46…”

Section: Spinal Cord Contusionmentioning

confidence: 99%

Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury

Heinzel

Swiadek

Ashmwe

et al. 2020

JoVE

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Peripheral and central nerve injuries are mostly studied in rodents, especially rats, given the fact that these animal models are both cost-effective and a lot of comparative data has been published in the literature. This includes a multitude of assessment methods to study functional recovery following nerve injury and repair. Besides evaluation of nerve regeneration by means of histology, electrophysiology, and other in vivo and in vitro assessment techniques, functional recovery is the most important criterion to determine the degree of neural regeneration. Automated gait analysis allows recording of a vast quantity of gait-related parameters such as Paw Print Area and Paw Swing Speed as well as measures of inter-limb coordination. Additionally, the method provides digital data of the rats' paws after neuronal damage and during nerve regeneration, adding to our understanding of how peripheral and central nervous injuries affect their locomotor behavior. Besides the predominantly used sciatic nerve injury model, other models of peripheral nerve injury such as the femoral nerve can be studied by means of this method. In addition to injuries of the peripheral nervous systems, lesions of the central nervous system, e.g., spinal cord contusion can be evaluated. Valid and reproducible data assessment is strongly dependent on meticulous adjustment of the hard-and software settings prior to data acquisition. Additionally, proper training of the experimental animals is of crucial importance. This work aims to illustrate the use of computerized automated gait analysis to assess functional recovery in different animal models of peripheral nerve injury as well as spinal cord contusion injury. It also emphasizes the method's limitations, e.g., evaluation of nerve regeneration in rats with sciatic nerve neurotmesis due to limited

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Section: Spinal Cord Contusionmentioning

confidence: 99%

Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury

Heinzel

Swiadek

Ashmwe

et al. 2020

JoVE

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“…Проблема виходжування передчасно народжених дітей на сьогодні набуває все більшої актуальності. Постнатальна трансформація життєво важливих систем у недоношених дітей в порівнянні з доношеними має суттєві відмінності, причому не тільки в тривалості процесу адаптації, а й в самих механізмах цього процесу [11,13]. Несприятливі умови внутрішньоутробного розвитку, постнатальні ушкодження, в тому числі і перинатальне ураження центральної нервової системи, як правило, порушують процеси становлення механізмів регуляції серцевосудинної системи [1,4,9].…”

Section: вступunclassified

Characteristics of Heart Rate in Late Premature Newborns With Perinatal Affection of Central Nervous System

Soloviova

2020

Act. Probl. of the Modern Med.

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The adaptation of late premature babies to the new life conditions is difficult and requires careful monitoring of all vital parameters in the postnatal period. The general immaturity of the newborns in combination with the metabolic and hypoxic disorders "leaves only a narrow corridor" to develop babies their compensatory possibilities. There is an urgent need to investigate posthypoxic myocardial ischemia in newborns due to the fact that in the neonatal period, early diagnosis and correct treatment can prevent long-term adverse consequences of existing disorders. The aim of this study was to develop an approach for early detection of cardiac rhythm disturbances and conduction disorders in late premature infants, who underwent perinatal hypoxia. A single-center study included 93 late premature babies who were born at the Perinatal Center, Poltava, in 2019 – 2020. Group I consisted of newborns (n = 47) with hypoxic-ischemic damage of the central nervous system; group II included premature babies (n = 46) with hypoxic-hemorrhagic damage of the central nervous system. Long-term monitoring of the electrocardiogram was performed with further conversion of the altered QRST-QRST complexes into 2D format with a multi-coloured representation of all components of the ventricular electrical systole. Among heterotopic cardiac arrhythmias, supraventricular extrasystoles were most often recorded in 89.4 ± 4.8% of the children of group I and in 67.4 ± 6.1% of newborns in group II with daytime distribution in both groups. Ventricular extrasystoles were found as significantly more frequent in newborns of group I (21.3 ± 6.3%) compared with children in group II (10.9 ± 6.1%), with a significant increase in the area of ectopic ventricular complexes (1492.2) that indicates a prolonged depolarization process. The study of the bioelectrical activity of the heart based on the findings obtained by monitoring the electrocardiogram with the qualitative and quantitative analysis of the convertible QRST-QRST complexes increases the efficiency of visual diagnosis of electrical instability of the myocardium in late premature infants with perinatal damage of the central nervous system.

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“…Voles have demonstrated unique contributions to understanding of social interactions, including in an understanding of the role of oxytocin in pair-bond formation in mammals 87 . Ferrets are an increasingly attractive species to model brain injury because of physical and developmental similarities to humans 88 and complex social and cognitive behavior. The ferret has a ratio of white-to-gray matter that is similar to humans as well as a gyrencephalic cerebral cortex, unlike rodents and rabbits.…”

Section: Normal Physiological Variations Should Guide Experimental Dementioning

confidence: 99%

“…Ferrets are born lissencephalic and develop gyrencephalic brains postnatally with white-matter maturation and cortical folding mirroring that of the third trimester of humans 89 . Therefore, the utility of ferrets in modeling perinatal brain injury or neurodevelopmental disorders is high, especially with the increase in validation of assays and behavioral assessments to evaluate therapeutic effect 88 . Ferrets have also been used extensively in immunology, especially influenza research, as their immunological responses more closely resemble those of humans than do rodents 90 .…”

Section: Normal Physiological Variations Should Guide Experimental Dementioning

confidence: 99%

Disease-directed engineering for physiology-driven treatment interventions in neurological disorders

Wood

Nance

2019

APL Bioengineering

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Neurological disease is killing us. While there have long been attempts to develop therapies for both acute and chronic neurological diseases, no current treatments are curative. Additionally, therapeutic development for neurological disease takes 15 years and often costs several billion dollars. More than 96% of these therapies will fail in late stage clinical trials. Engineering novel treatment interventions for neurological disease can improve outcomes and quality of life for millions; however, therapeutics should be designed with the underlying physiology and pathology in mind. In this perspective, we aim to unpack the importance of, and need to understand, the physiology of neurological disease. We first dive into the normal physiological considerations that should guide experimental design, and then assess the pathophysiological factors of acute and chronic neurological disease that should direct treatment design. We provide an analysis of a nanobased therapeutic intervention that proved successful in translation due to incorporation of physiology at all stages of the research process. We also provide an opinion on the importance of keeping a high-level view to designing and administering treatment interventions. Finally, we close with an implementation strategy for applying a disease-directed engineering approach. Our assessment encourages embracing the complexity of neurological disease, as well as increasing efforts to provide system-level thinking in our development of therapeutics for neurological disease.

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A Ferret Model of Encephalopathy of Prematurity

Cited by 9 publications

References 37 publications

Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury

Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury

Characteristics of Heart Rate in Late Premature Newborns With Perinatal Affection of Central Nervous System

Disease-directed engineering for physiology-driven treatment interventions in neurological disorders

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