A feline model for experimental studies of peritumor brain edema

Abstract

An in vivo model for correlative imaging studies of intracerebral glial tumors and peritumor brain edema has been developed. Adult male and female cats implanted with 1 x 10(6) or 5 x 10(5) 9L glioma cells had parietal tumors of 4 mm or greater in diameter and showed signs of increased intracranial pressure 13.7 +/- 1.9 days or 19.2 +/- 1.3 days after implantation. No immunosuppression was required and the success rate for tumor growth after implantation was 88%. Histologically, the tumor resembles a malignant astrocytoma. The tumor contained the highest water content (85.94%); peritumor white matter was more edematous (73.01%) than white matter in the contralateral hemisphere (69.04%), sham-operated (69.41%) and control brain (68.76%). There was no correlation between the size of the tumor and water content in tumor or white matter. Increased tissue albumin in peritumor white matter indicated blood-brain barrier dysfunction within the tumor and confirmed the vasogenic origin of the edema. Proton magnetic resonance imaging provided good spatial and contrast resolution with increased signal intensity in edematous white matter, decreasing with distance from the tumor. The large brain of this animal model allows the use of serial imaging and regional correlative biochemical measurements in a single animal. Other advantages of this model are its predictability and the short time required to produce tumors with marked peritumor edema.

A novel rat model for glioblastoma multiforme using a bioluminescent F98 cell line

“…1 Mouse, 2 cat 3 and dog 4,5 models have been utilized previously, but the size limitations of the former and the increased costs for the latter two 6,7 have limited the widespread utility of these models. However, despite extensive experimentation, there is no one rat model that truly reflects the features of human tumours.…”

Use of anti-coronavirus antibody testing of cerebrospinal fluid for diagnosis of feline infectious peritonitis involving the central nervous system in cats

“…50,51 Blood-brain barrier dysfunction has been described in cats with experimentally induced astrocytomas. 52 Similarly, meningiomas can break into the Virchow-Robin space and disrupt the blood-brain barrier. 53 Uremic encephalopathy, necrosis, and oculomotor tract degeneration are less likely to be associated with a disrupted blood-brain barrier than brain tumors and inflammatory disease; therefore, the absence of CSF antibodies is not unexpected in those cats.…”

Zebrafish—A Suitable Model for Rapid Translation of Effective Therapies for Pediatric Cancers

“…A number of animal models have been established for studying pediatric tumors over the past six decades. Invertebrates, including C. elegans and Drosophila, to vertebrates, such as rodents, felines, canines, and non-human primates [25][26][27][28][29], have been utilized to evaluate potential therapeutic strategies and cures for this deadly disease. Rodent models, particularly mice and rats, are the most used animal models in pediatric cancer research.…”