A feedforward–feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase

Arndt, M.; Kleist, Sofia; Miksch, Gerhard; Friehs, Karl; Flaschel, Erwin; Trierweiler, Jorge Otávio; Hitzmann, Bernd

doi:10.1016/j.compchemeng.2004.11.011

Cited by 29 publications

(11 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Classical proportional or proportional-integral controllers cannot cope with the exponential nature of both process kinetics and disturbances and therefore, require dynamic parameter tuning or gain scheduling [2,4,5]. However, gain adaptation is inherently process specific and may lead to decreased robustness of the controller and its weak applicability to other organism strains or process conditions [18].…”

Section: Introductionmentioning

confidence: 99%

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

Dabros

Schuler

Marison

2010

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Reliable control of the specific growth rate (μ) in fed-batch fermentations depends on the availability of accurate online estimations of the controlled variable. Due to difficulties in measuring biomass, μ is typically estimated using reference models relating measurements of substrate consumption or oxygen uptake rate to biomass growth. However, as culture conditions vary, these models are adapted dynamically, resulting in complex algorithms that lack the necessary robustness for industrial applicability. A simpler approach is presented where biomass is monitored using dielectric spectroscopy. The measurements are subjected to online balances and reconciled in real time against metabolite concentrations and off-gas composition. The reconciled biomass values serve to estimate the growth rate and a simple control scheme is implemented to maintain the desired value of μ. The methodology is developed with the yeast Kluyveromyces marxianus, tested for disturbance rejection and validated with two other strains. It is applicable to other cellular systems with minor modifications.

show abstract

Section: Introductionmentioning

confidence: 99%

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

Dabros

Schuler

Marison

2010

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…Es wird versucht, durch kurze Probenwege at line schnelle Aussagen zu machen. So ist es möglich durch eine Fließ-injektions-Analyse (FIA) unter Verwendung eines Kalmanfilters, den Verbrauch an Substrat und damit die Zunahme der Biomasse zu berechnen [51].…”

Section: Indirekte Methodenunclassified

Bioprozessanalytik

Rehbock¹,

Beutel²,

Brückerhoff³

et al. 2008

Chemie Ingenieur Technik

Self Cite

View full text Add to dashboard Cite

“…Indeed, as highlighted by Gnoth et al (2008): "feedback control only makes sense where the current value of the controlled variable can be determined accurately and fast enough to determine the deviation from the target set-point." The adaptability of the organisms makes the control task even more difficult as pointed out by Arndt et al (2005). However, Table 3 as well as Figs.…”

Section: Controlling the Specific Growth Rate In Bioprocessesmentioning

confidence: 96%

Real-time monitoring and control of microbial bioprocesses with focus on the specific growth rate: current state and perspectives

Schuler

Marison

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Understanding the growth characteristics of microorganisms is an essential step in bioprocessing, not only because product formation may be growth-associated but also because they might influence cell physiology and thereby product quality. The specific growth rate, a key variable of many bioprocesses, cannot be measured directly and relies on the estimation through other measurable variables such as biomass, substrate, or product concentrations. Techniques for real-time estimation of the specific growth rate in microbial fed-batch cultures are discussed in the present paper. The advantages and limitations of different models and various monitoring techniques are discussed, highlighting the importance of the specific growth rate in the development of fast, reliable, and robust processes for the production of high-value products such as recombinant proteins.

show abstract

A feedforward–feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase

Cited by 29 publications

References 16 publications

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

Bioprozessanalytik

Real-time monitoring and control of microbial bioprocesses with focus on the specific growth rate: current state and perspectives

Contact Info

Product

Resources

About