A feasibility study of singlet oxygen explicit dosmietry (SOED) of PDT by intercomparison with a singlet oxygen luminescence dosimetry (SOLD) system

Kim, Michele M.; Penjweini, Rozhin; Gemmell, Nathan R.; Veilleux, Israël; McCarthy, Aongus; Buller, Gerald S.; Hadfield, Robert H.; Wilson, Brian C.; Zhu, Timothy C.

doi:10.1117/12.2213236

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…Currently, there are no in vivo capable singlet O 2 dosimeters used during PDT treatment, so these key parameters of PDT treatment are unquantifiable to physicians in the clinic. Evidence increasingly indicates that treatment outcome and variation have a strong correlation with the amount of singlet O 2 produced within the tumor. − Thus, accurate singlet O 2 dosimetry would have a significant impact on the advancement of PDT in cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

Singlet Molecular Oxygen: from COIL Lasers to Photodynamic Cancer Therapy

Davis

Zhao

et al. 2023

J. Phys. Chem. B

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Translation of experimental techniques from one scientific discipline to another is often difficult but rewarding. Knowledge gained from the new area can lead to long lasting and fruitful collaborations with concomitant development of new ideas and studies. In this Review Article, we describe how early work on the chemically pumped atomic iodine laser (COIL) led to the development of a key diagnostic for a promising cancer treatment known as photodynamic therapy (PDT). The highly metastable excited state of molecular oxygen, a1Δg, also known as singlet oxygen, is the link between these disparate fields. It powers the COIL laser and is the active species that kills cancer cells during PDT. We describe the fundamentals of both COIL and PDT and trace the development path of an ultrasensitive dosimeter for singlet oxygen. The path from COIL lasers to cancer research was relatively long and required medical and engineering expertise from numerous collaborations. As we show below, the knowledge gained in the COIL research, combined with these extensive collaborations, has resulted in our being able to show a strong correlation between cancer cell death and the singlet oxygen measured during PDT treatments of mice. This progress is a key step in the eventual development of a singlet oxygen dosimeter that could be used to guide PDT treatments and improve outcomes.

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Section: Introductionmentioning

confidence: 99%

Singlet Molecular Oxygen: from COIL Lasers to Photodynamic Cancer Therapy

Davis

Zhao

et al. 2023

J. Phys. Chem. B

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“…Accumulating evidence indicates that the variations in treatment outcome have a strong correlation with the amount of 1 O 2 produced within the tumor. [11][12][13][14][15][16] Several methodologies have been investigated for PDT dosimetry. [17][18][19] In principle, an explicit dosimeter that quantifies all the contributing parameters would be ideal.…”

Section: Introductionmentioning

confidence: 99%

“…11,12,20 Extensive in vivo studies and very promising results on this topic have been published. [11][12][13] For example, using an explicit model, one study calculated 1 O 2 concentrations at different radial distances from the excited benzoporphyrin derivative monoacid ring A (BPD). These calculations have the potential to be used to determine pretreatment patientspecific parameters to predict and optimize personalized PDT outcome.…”

Section: Introductionmentioning

confidence: 99%

Multispectral singlet oxygen and photosensitizer luminescence dosimeter for continuous photodynamic therapy dose assessment during treatment

et al. 2020

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Significance: Photodynamic therapy (PDT) involves complex light-drug-pathophysiology interactions that can be affected by multiple parameters and often leads to large variations in treatment outcome from patient to patient. Direct PDT dosimetry technologies have been sought to optimize the control variables (e.g., light dose, drug administration, tissue oxygenation, and patient conditioning) for best patient outcomes. In comparison, singlet oxygen (1 O 2) dosimetry has been tested in various forms to provide an accurate and perhaps comprehensive prediction of the treatment efficacy. Aim: We discuss an advanced version of this approach provided by a noninvasive, continuous wave dosimeter that can measure near-infrared spectrally resolved luminescence of both photosensitizer (PS) and 1 O 2 generated during PDT cancer treatment. Approach: This dosimetry technology uses an amplified, high quantum efficiency InGaAs detector with spectroscopic decomposition during the light treatment to continuously extract the maximum signal of 1 O 2 phosphorescence while suppressing the strong PS luminescence background by spectrally fitting the data points across nine narrow band wavelengths. 1 O 2 and PS luminescence signals were measured in vivo in FaDu xenograft tumors grown in mice during PDT treatment using Verteporfin as the PS and a continuous laser treatment at 690 nm wavelength. Results: A cohort of 19 mice was used and observations indicate that the tumor growth rate inhibition showed a stronger correlation with 1 O 2 than with just the PS signal. Conclusions: These results suggest that 1 O 2 measurement may be a more direct dosimeter of PDT damage, and it has potential value as a definitive diagnostic for PDT treatment, especially with spectral separation of the background luminescence and online estimation of the PS concentration.

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“…The use of PDI in medicine is becoming more relevant, since it is a reality in the treatment of diseases such as cancer, periodontitis, dermatologic diseases such as actinic keratosis and common acne, venereal diseases such as acuminate condyloma, among others ( 11 - 13 ).…”

Section: Introductionmentioning

confidence: 99%

Use of photodynamic inactivation for in vitro reduction of prevalent bacteria in Fournier's Gangrene

et al. 2018

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Fournier's Gangrene (FG) is an infectious disease caused by several synergic microbes, with high morbidity and mortality rates; therefore, the search for new less invasive and mutilating treatments, with faster recovery, has been proposed. Surgical intervention, the use of several systemic and topic antibiotics, and hyperbaric oxygen therapy are currently the best approach for the treatment of these patients. The use of Photodynamic Inactivation (PDI) aims to lower morbidity and mortality, by reducing bacterial microbiota and speeding wound healing. In the present study, viable bacteria were separated in four groups: Group L-/F- (no irradiation with red laser and absence of methylene blue photosensitizer), Group L-/F+ (no irradiation with red laser and presence of methylene blue), Group L+/F- (irradiation with red laser and absence of methylene blue) and L+/F+ (irradiation with red laser associated to methylene blue). In all groups, exposure time to treatment was 5, 10 and 15 minutes. The concentration of methylene blue photosensitizer was 0.1mg/L, and the dose of red laser (660nm wave length) was 176.9mW/cm2. Following irradiation, the reduction of number of bacteria was evaluated, and the results were expressed in colony forming units (CFU) and as exponential reduction. As the main results, in the L+/F+ group, there were no Clostridium perfringens and Staphylococcus aureus CFUs and there was a reduction of Escherichia coli that was not observed in the other groups.

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A feasibility study of singlet oxygen explicit dosmietry (SOED) of PDT by intercomparison with a singlet oxygen luminescence dosimetry (SOLD) system

Cited by 4 publications

References 42 publications

Singlet Molecular Oxygen: from COIL Lasers to Photodynamic Cancer Therapy

Singlet Molecular Oxygen: from COIL Lasers to Photodynamic Cancer Therapy

Multispectral singlet oxygen and photosensitizer luminescence dosimeter for continuous photodynamic therapy dose assessment during treatment

Use of photodynamic inactivation for in vitro reduction of prevalent bacteria in Fournier's Gangrene

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