A Feasibility Research on Restoration of Demyelination in Trigeminal Nerve with Transnasal Administration of BDNF/ NGF Loading on PEG-PLA Nanoparticles

Abstract

Background Trigeminal neuralgia (TN) is caused by the vascular compression of the Vth cranial nerve root, where the pathology of demyelination has been observed. Although microvascular decompression surgery has become the most reasonable technique for the treatment, this sort of operation is with risk and invasive. Accordingly, we attempt to find a noninvasive approach to treat TN. Preparation of PEG-PLA nanoparticles loaded with BDNF or NGF PEG-PLA nanoparticles (NP) were prepared by the emulsion/solvent evaporation method. Briefly, MePEG-PLA (22.5 mg) and Male-PEG-PLA (2.5 mg) were gifted from Sichuan university of electronic science and technology and were dissolved in 1 ml of dichloromethane and added The CCK-8 and Immunostaining of S-100 experiments indicated that the BDNF-NPs or NGF-NPs could promote the expression of Schwann cells (p<0.05). Conclusions The study demonstrated that BDNF/NGF loading on nanoparticles may transmit along with the trigeminal nerve via nasal administration and facilitate the restoration of myelin sheath. It could be a sort of noninvasive remedy for trigeminal neuralgia.