A fatty acyl-CoA reductase highly expressed in the head of honey bee (Apis mellifera) involves biosynthesis of a wide range of aliphatic fatty alcohols

Teerawanichpan, Prapapan; Robertson, A.J.; Qiu, Xiao

doi:10.1016/j.ibmb.2010.06.004

Cited by 77 publications

(81 citation statements)

References 41 publications

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“…The majority of information is available for one insect group -the moths [18]. FA-modifying enzymes from other insect orders, such as beetles (Coleoptera) [26], flies (Diptera) [27][28][29], crickets (Orthoptera) [30,31] and bees (Hymenoptera) [32][33][34], are substantially less well explored and usually connected to primary FA metabolism rather than pheromone biosynthesis. Two enzyme groups that are encoded by large multigene families in insects -the membrane fatty acyl desaturases (mFADs) [35,36] and the fatty acyl reductases (FARs) [37] -have attracted the most research attention.…”

Section: Insect Enzymes Catalyzing Biosynthesis Of Fa-derived Pheromonesmentioning

confidence: 99%

“…FARs from insects other than moths are virtually unexplored. A FAR presumably involved in biosynthesis of fatty alcohol precursors of waxes has been identified in Drosophila [29], and honeybee (Apis mellifera) FAR capable of reducing hydroxylated fatty acyl precursors and saturated C16-C22 fatty acyls has been characterized [34]. FARs from pheromone-producing glands in the butterfly Bicyclus anynana reduce C14 and C16 fatty acyls [86].…”

Section: Far Propertiesmentioning

confidence: 99%

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Biotechnological potential of insect fatty acid-modifying enzymes

Tupec

Buček

Valterová

2017

Zeitschrift Für Naturforschung C

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There are more than one million described insect species. This species richness is reflected in the diversity of insect metabolic processes. In particular, biosynthesis of secondary metabolites, such as defensive compounds and chemical signals, encompasses an extraordinarily wide range of chemicals that are generally unparalleled among natural products from other organisms. Insect genomes, transcriptomes and proteomes thus offer a valuable resource for discovery of novel enzymes with potential for biotechnological applications. Here, we focus on fatty acid (FA) metabolism-related enzymes, notably the fatty acyl desaturases and fatty acyl reductases involved in the biosynthesis of FA-derived pheromones. Research on insect pheromone-biosynthetic enzymes, which exhibit diverse enzymatic properties, has the potential to broaden the understanding of enzyme specificity determinants and contribute to engineering of enzymes with desired properties for biotechnological production of FA derivatives. Additionally, the application of such pheromone-biosynthetic enzymes represents an environmentally friendly and economic alternative to the chemical synthesis of pheromones that are used in insect pest management strategies.

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Section: Insect Enzymes Catalyzing Biosynthesis Of Fa-derived Pheromonesmentioning

confidence: 99%

Section: Far Propertiesmentioning

confidence: 99%

Biotechnological potential of insect fatty acid-modifying enzymes

Tupec

Buček

Valterová

2017

Zeitschrift Für Naturforschung C

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“…Primary fatty alcohols and their derivatives have a wide range of biological roles in bacteria, fungi, insects, plants, and animals. Some important functions of free and esterified fatty alcohols in nonplant organisms are as follows: 1) as an energy storage reserve in microorganisms such as Euglena gracilis, Acinetobacter sp., and some marine microalgae; 2) as a constituent of sex pheromones in some insect and reptile species; 3) as a uropygial gland secretion in birds to maintain feather condition; 4) in the heads of sperm whales for echolocation and possibly to regulate buoyancy; and 5) as ether lipids, which play a variety of roles and are highly abundant in animal nervous system tissue (2)(3)(4)(5)(6)(7)(8)(9)(10). In plants, fatty alcohols and their derivatives are found primarily as chemical constituents of three extracellular lipid-phenolic barriers as follows: cuticle coating the aerial surfaces of plants, suberin present in the cell walls of various external and internal tissue layers, and sporopollenin found in the outermost layer of the pollen spore coat (1,11).…”

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confidence: 99%

Identification of Amino Acids Conferring Chain Length Substrate Specificities on Fatty Alcohol-forming Reductases FAR5 and FAR8 from Arabidopsis thaliana

Chacón

Fournier

Tran

et al. 2013

Journal of Biological Chemistry

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Background: Fatty alcohols produced by fatty acyl reductases (FARs) have important biological roles. Results: Expression of Arabidopsis FAR5 and FAR8 mutants in yeast revealed amino acids important for their stability or substrate specificity. Conclusion: Amino acids 355 and 377 are 16:0 versus 18:0 chain length specificity determinants of FAR5 and FAR8. Significance: Engineered FAR enzymes with desired substrate specificities will enable production of high value products.

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“…Finding reductase genes potentially affecting CHC production resonates with other studies in both plants (WANG and KOLATTUKUDY 1995;ROWLAND et al 2006) and animals (TURGEON and BERNATCHEZ 2001;MOTO et al 2003;LASSANCE et al 2010;TEERAWANICHPAN et al 2010). Except for a recent study that used an RNAi knock-down approach to confirm the role of decarbonylating reductase genes in CHC biosynthesis in Drosophila (TURGEON and BERNATCHEZ 2001), most of the studies that relate fatty-acyl reductase genes as affecting hydrocarbon biosynthesis in animals are not in Drosophila but in Ostrinia nubilalis moths , silkworm Bombyx mori (MOTO et al 2003) and honey bees, Apis mellifera (TEERAWANICHPAN et al 2010).…”

Section: The Genetic Basis Of D Serrata Chcsmentioning

confidence: 78%

“…Except for a recent study that used an RNAi knock-down approach to confirm the role of decarbonylating reductase genes in CHC biosynthesis in Drosophila (TURGEON and BERNATCHEZ 2001), most of the studies that relate fatty-acyl reductase genes as affecting hydrocarbon biosynthesis in animals are not in Drosophila but in Ostrinia nubilalis moths , silkworm Bombyx mori (MOTO et al 2003) and honey bees, Apis mellifera (TEERAWANICHPAN et al 2010). This is the first study that has mapped changes in CHCs in a Drosophila species to natural variation in biosynthetically relevant fatty-acyl-CoA reductase genes; most of the prior research in Drosophila has largely focused on desaturases and elongases (TAKAHASHI et al 2001).…”

Section: The Genetic Basis Of D Serrata Chcsmentioning

confidence: 99%

Genetic dissection of a major polymorphism underlying population divergence in sexually selected pheromones in Drosophila serrata

Rusuwa¹

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With the advent of QTL mapping in the late 1980s, evolutionary geneticists have actively dissected the genetic basis of adaptive phenotypes. In recent times, advances in whole genome sequencing have allowed for finer scale mapping of traits that have diverged due to natural selection. These relatively inexpensive sequencing technologies have also greatly facilitated adaptive trait dissection in non-model species that exhibit especially interesting patterns of adaptation. One class of trait that has largely escaped the attention of the next generation ecological and evolutionary genomics research programme, are sexually-selected traits. Sexual selection is a strong form of directional selection in nature and is thought to be responsible for the evolution of elaborate sexual ornaments and armaments. However we still know comparatively little of the genetic basis of such traits.The cuticular hydrocarbons (CHCs) of Drosophila serrata provide an opportunity for dissecting the divergence of sexually selected traits. Populations along the eastern Australian coast exhibit latitudinal variation in these traits in association with climatic factors and consistent with the action of divergent natural selection. Under experimental settings, divergent sexual as well as natural selection has been implicated in the evolution of CHCs. Natural populations of D. serrata along the northern part of the Eastern Australian coast have recently been observed to exhibit a polymorphism in three of their CHC compounds representing the shortest carbon chains; 5,9-tetracosadiene (5,9-C 24 ), 5,9-pentacosadiene (5,9-C 25 ) and 9-pentacosene (9-C 25 ). One class of phenotype only expresses these three compounds in trace amounts ('low' phenotype) whereas the other has normal levels ('high' phenotype). These short-chained CHCs also exhibit strong genetically-based latitudinal clines up to, but not beyond, 20 degrees south of the equator. Based on both traditional QTL and modern sequence-based genomic mapping approaches, the aim of this study was to dissect the genetic basis of the polymorphism in D. serrata CHCs and to examine its adaptive significance within the context of sexual and natural selection.Through F2 QTL mapping based on a cross between two inbred lines, from the opposite ends of the D. serrata CHC cline, one a 'low' phenotype and the other a 'high' phenotype, the genetic basis of all CHCs in this species was mapped to twenty two overlapping QTLs on chromosomes 2 and 3.The short-chain CHC polymorphism was traced to two major effect recessive QTLs on the right arm of chromosome 3, which including their interaction, accounted for more than 70% of the variance in the polymorphism (Chapter 2). Fine mapping of this major polymorphism was then conducted using next generation DNA sequencing and bulk segregant analysis of an advanced F60 cross of the same founding lines. Bulk segregant analysis revealed a single peak of genetic differentiation on chromosome 3R (~20kb), harbouring three adjacent fatty acyl-CoA reductase ii genes (Cha...

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A fatty acyl-CoA reductase highly expressed in the head of honey bee (Apis mellifera) involves biosynthesis of a wide range of aliphatic fatty alcohols

Cited by 77 publications

References 41 publications

Biotechnological potential of insect fatty acid-modifying enzymes

Biotechnological potential of insect fatty acid-modifying enzymes

Identification of Amino Acids Conferring Chain Length Substrate Specificities on Fatty Alcohol-forming Reductases FAR5 and FAR8 from Arabidopsis thaliana

Genetic dissection of a major polymorphism underlying population divergence in sexually selected pheromones in Drosophila serrata

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