A faster reconstitution of hematopoiesis after autologous transplantation of hematopoietic cells cryopreserved in 7.5% dimethyl sulfoxide if compared to 10% dimethyl sulfoxide containing medium

Effect of high nucleated cell concentration on product viability and hematopoietic recovery in autologous transplantation

“…1 In spite of the first report by Rowley, the optimal NC concentration of cryopreserved HPC(A) products has remained controversial as the majority of the recently published literature suggests that this optimal concentration should be less than 3 × 10 8 NC/mL. [2][3][4][5][6][7][8] Administering lower total product volumes with high NC concentrations, however, may have the potential benefit of decreasing volume-and dimethyl sulfoxide (DMSO)-related patient complications, while maximizing the laboratoryʼs liquid nitrogen (LN2) freezer storage capacity. Our study is a retrospective investigation of the effect of HPC(A) products with cell concentrations greater than 3 × 10 8 NC/mL on clinical and product outcomes in patients undergoing autologous PBSC transplantation.…”

Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes

“…A study by Hayakawa et al [28] used 5% DMSO and 5% pentastarch in the cryopreservation of UCB. Cells in 5% DMSO with pentastarch had higher post-thaw viability than cells frozen in 10% DMSO [26]. McCullough et al [29] explored the use of hydroxyethyl starch (HES) mixed with DMSO at different ratios to enhance cryopreservation of PBSCs.…”

“…A subsequent clinical study compared 7.5 and 10% DMSO among two groups of patients. Reduction of DMSO resulted in faster leukocyte recovery, but the frequency of adverse events was unchanged between the two groups [26]. In the following 2018 clinical study, autologous peripheral blood stem cell (PBSC) transplants of cells frozen at all three DMSO concentrations were performed in 150 patients [27].…”

Comparison of the cryoprotective solutions based on human albumin vs. autologous plasma: its effect on cell recovery, clonogenic potential of peripheral blood hematopoietic progenitor cells and engraftment after autologous transplantation

“…Differences regard many factors that have been demonstrated to affect the quality of the hematopoietic stem/progenitor cells after thawing. These are as follows: cell storage conditions before cryopreservation [23][24][25][26], cooling rate of cells (freezing curve) [7,18,26,27], temperature and speed of adding cryoprotective mixture [24][25][26] and final DMSO and cell concentration [21,[28][29][30][31][32][33][34][35][36]. In particular, in our previous studies, we demonstrated that cryopreservation with 7Á5% DMSO was associated with higher clonogenic potential and faster post-transplant engraftment compared with 10% DMSO [21,29].…”