We report the construction of a spectroscopic programmable array microscope capable of optical sectioning using H adamard transform methods. Previously described H adamard transform imaging consisted of rst-order systems in which the m ask was used for either illumination or detection. In this paper, a second-order Hadamard transform system is described in which a single one-dimensional mask serves for both illumination and detection. The system veri es earlier predictions that the optical spreading function along the transform axis can be inferred from the echoes arising in second-order systems using sequence lengths of 2 n -1. W ith a one-dimensional H adamard mask, the system has an axial resolution of 1.3 m m and may be used with or without the subtraction of an appropriately scaled wide eld image depending on the degree of sectioning required. The system was tested using thin lms, uorescent beads, and a xed biological specimen.