A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos

Somkhit, Julie; Loyant, Roxane; Brenet, Alexandre; Hassan-Abdi, Rahma; Yanicostas, Constantin; Porceddu, Mathieu; Borgne‐Sanchez, Annie; Soussi-Yanicostas, Nadia

doi:10.1089/zeb.2020.1878

Cited by 4 publications

(3 citation statements)

References 5 publications

(8 reference statements)

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“…Therefore, these cellular organelles play an essential role in generating intracellular ROS, being responsible for 90% of their endogenous production [ 36 , 37 ]. A cell’s oxygen consumption measurement is probably the most informative parameter for assessing its metabolism, because it allows a direct and specific evaluation of the functioning of the electron transport chain underlying oxidative phosphorylation and cellular metabolism [ 38 , 39 , 40 ]. Traditionally, oxygen consumption in isolated mitochondria and whole cells was performed using a Clark electrode [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…A cell’s oxygen consumption measurement is probably the most informative parameter for assessing its metabolism, because it allows a direct and specific evaluation of the functioning of the electron transport chain underlying oxidative phosphorylation and cellular metabolism [ 38 , 39 , 40 ]. Traditionally, oxygen consumption in isolated mitochondria and whole cells was performed using a Clark electrode [ 40 ]. Currently, convenient and rapid methods using fluorescent probes to measure extracellular oxygen concentration are used for this purpose, whereby average values can be monitored in areas immediately surrounding the cell after sealing the cell suspension with mineral oil [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Platelets in Fetal Growth Restriction: Role of Reactive Oxygen Species, Oxygen Metabolism, and Aggregation

Nowaczyk

Poniedziałek

Rzymski

et al. 2022

Cells

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Fetal growth restriction (FGR) is mainly caused by failure of the uteroplacental unit. The exact pathogenesis remains unclear. The cause is thought to be related to abnormal platelet activation, which may result in microthrombus formation in the small vessels of the placenta. Reactive oxygen species (ROS) may initiate the pathological process of platelet activation. This study aimed to evaluate selected platelet parameters in pregnancy complicated by FGR and relate them to the severity of hemodynamic abnormalities. A total of 135 women (pregnant with FGR, with an uncomplicated pregnancy, and non-pregnant) were enrolled to study different platelet parameters: count (PLT), mean volume (MPV), ROS levels, intracellular oxygen level, oxygen consumption, and aggregation indices. No abnormalities in PLT and MPV were found in the FGR group, although it revealed increased ROS levels in platelets, lower platelet oxygen consumption, and intraplatelet deprivation. Aggregation parameters were similar as in uncomplicated pregnancy. No significant relationships were observed between hemodynamic abnormalities and the studied parameters. Platelets in pregnancies complicated by FGR may reveal an impaired oxidative metabolism, which may, in turn, lead to oxidative stress and, consequently, to an impaired platelet function. This study adds to the understanding of the role of platelets in the etiology of FGR.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Platelets in Fetal Growth Restriction: Role of Reactive Oxygen Species, Oxygen Metabolism, and Aggregation

Nowaczyk

Poniedziałek

Rzymski

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…The wells with the oxygen probe but without cells were used as blank to calculate the time-dependent change of relative fluorescence intensity and area under the curve (AUC). Oxygen consumption was calculated referring to a previous report (24), with a slight modification using the following equation: Oxygen consumption (relative to control)5 [AUCtest group2AUCblank]/[AUCcontrol group2 AUCblank].…”

Section: Methodsmentioning

confidence: 99%

Near-Physiological Concentrations of Extracellular Pyruvate Stimulated Glucose Utilization along with Triglyceride Accumulation and Mitochondrial Activity in HepG2 Cells

KURAWAKA,

SASAKI,

YAMAZAKI

et al. 2023

J Nutr Sci Vitaminol

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Pyruvate, a key intermediate in energy and nutrient metabolism, probably plays important roles in these regulations. In previous reports using cell lines, extracellular pyruvate of supraphysiological concentrations inhibited the glucose uptake by myotubes while being stimulated by adipocytes. As the effect of pyruvate on the glucose utilization is unclear in cultured hepatocytes. We have investigated the effects of extracellular pyruvate on the glucose utilization and the subsequent metabolic changes using the cell line HepG2. In a 24 h culture, pyruvate enhanced the glucose consumption more potently than 1 mM insulin, and this enhancement was detectable at a near-physiological concentrations of #1 mM. For metabolic changes following glucose consumption, the conversion ratio of glucose and pyruvate to extracellular lactate was approximately 1.0 without extracellular pyruvate. The addition of pyruvate decreased the conversion ratio to approximately 0.7, indicating that the glycolytic reaction switched from being an anaerobic to a partially aerobic feature. Consistent with this finding, pyruvate increased the accumulation of intracellular triglycerides which are produced through substrate supply from the mitochondria. Furthermore, pyruvate stimulated mitochondria activity as evidenced by increases in ATP content, mitochondrial DNA copy number, enhanced mitochondria-specific functional imaging and oxygen consumption. Interestingly, 1 mM pyruvate increased oxygen consumption immediately after addition. In this study, we found that near-physiological concentrations of extracellular pyruvate exerted various changes in metabolic events, including glucose influx, lactate conversion rations, TG accumulation, and mitochondrial activity in HepG2 cells.

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Inhibition of Xenobiotics Transporters’ Efflux Ability after Nanoplastics Exposure in Larval Japanese Medaka

Gao

Yang

et al. 2022

Water

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Nanoplastics can enter into the aquatic environment as primary nano-sized or fragmented from larger-sized plastic particles, and their ecological effects and environmental fate have aroused increasing public concerns. Here, we identified the disruption of ATP-binding cassette (ABC) efflux after polystyrene (PS) nanoplastics (76 ± 7 nm) exposure in larval Japanese medaka (Oryzias latipes). Nanoplastics (0.001–10 μg/mL) caused 3–6-fold higher lipid peroxidation in fish larvae than the control, with concomitant downregulated expression of efflux transporter-related genes (abcb6a, abcc2, abcg2). Two probes of rhodamine (indicative of p-glycoprotein function for parent compounds’ efflux, P-gp) and fluorescein (indicative of multidrug resistance-associated protein function for metabolites’ efflux, MRP) were further used to verify the inhibited ABC efflux ability, via rhodamine and fluorescein bioaccumulation results. Three-fold higher accumulation of rhodamine was observed following treatment with 10 μg/mL of nanoplastics. Excessive accumulation also occurred for fluorescein, with 1.7–1.8-fold higher concentrations than controls in larvae treated with 0.01–0.1 μg/mL of nanoplastics. Although the inhibition of ABC transporters diminished after two hours of depuration, the co-existence of nanoplastics and other contaminants still raises concerns. Collectively, this study suggests that nanoplastics can negatively impact ABC transporters’ efflux ability and could cause unanticipated accumulation of co-existing organic pollutants in aquatic organisms.

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A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos

Cited by 4 publications

References 5 publications

Platelets in Fetal Growth Restriction: Role of Reactive Oxygen Species, Oxygen Metabolism, and Aggregation

Platelets in Fetal Growth Restriction: Role of Reactive Oxygen Species, Oxygen Metabolism, and Aggregation

Near-Physiological Concentrations of Extracellular Pyruvate Stimulated Glucose Utilization along with Triglyceride Accumulation and Mitochondrial Activity in HepG2 Cells

Inhibition of Xenobiotics Transporters’ Efflux Ability after Nanoplastics Exposure in Larval Japanese Medaka

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