A fast scan cyclic voltammetric digital circuit with precise ohmic drop compensation by online measuring solution resistance and its biosensing application

Xiao, Fengming; Zhou, Huiqian; Lin, Han; Li, Hongze; Zou, Tinglang; Wu, Yangbo; Guo, Zhiyong

doi:10.1016/j.aca.2021.338744

Cited by 3 publications

(2 citation statements)

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“…To realize the rapid field detection of GMOs, a portable equipment with both ECL and FSV testing capabilities is still required, which is being developed in our group. 50,51 ■ CONCLUSIONS In summary, a dual-mode electrochemical biosensor for the detection of genetically modified soybean was developed based on the CRISPR/Cas12a system, which has significant advantages in the following aspects: (1) High sensitivity the LOD was as low as 0.3 fmol/L, affording the detection of low-concentration genetically modified ingredients. (2) High selectivitycommon interferences will not affect the detection.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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A CRISPR/Cas12a-Mediated Dual-Mode Electrochemical Biosensor for Polymerase Chain Reaction-Free Detection of Genetically Modified Soybean

Wang

Lin

et al. 2021

Anal. Chem.

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A clustered regularly interspaced short palindromic repeats (CRISPR)/ Cas12a-mediated dual-mode electrochemical biosensor without polymerase chain reaction (PCR) amplification was designed for sensitive and reliable detection of genetically modified soybean SHZD32-1. A functionalized composite bionanomaterial Fe 3 O 4 @AuNPs/DNA-Fc&Ru was synthesized as the signal unit, while a characteristic gene fragment of SHZD32-1 was chosen as the target DNA (tDNA). When Cas12a, crRNA, and tDNA were present simultaneously, a ternary complex Cas12a-crRNA-tDNA was formed, and the nonspecific cleavage ability of the CRISPR/Cas12a system toward single-stranded DNA was activated. Thus, the single-stranded DNA-Fc in the signal unit was cleaved, resulting in the decrease in the fast scan voltammetric (FSV) signal from ferrocene (Fc) and the increase in the electrochemiluminescence (ECL) signal from ruthenium complex (Ru) inhibited by Fc. The linear range was 1−10 7 fmol/L for ECL and 10−10 8 fmol/L for FSV, and the limit of detection (LOD) was 0.3 fmol/L for ECL and 3 fmol/L for FSV. Accuracy, precision, stability, selectivity, and reliability were all satisfied. In addition, PCR-free detection could be completed in an hour at room temperature without requiring complicated operation and sample processing, showing great potential in the field detection of genetically modified crops.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…To realize the rapid field detection of GMOs, a portable equipment with both ECL and FSV testing capabilities is still required, which is being developed in our group. , …”

Section: Resultsmentioning

confidence: 99%