A fast-response, highly specific fluorescent probe for the detection of picomolar hypochlorous acid and its bioimaging applications

Zhu, Baocun; Liu, Wu; Zhang, Meng; Wang, Yawei; Zhao, Ziyang; Wang, Zuokai; Duan, Qingxia; Jia, Pan; Liu, Caiyun

doi:10.1016/j.snb.2018.02.083

Cited by 69 publications

(14 citation statements)

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“…A new peak at 604 nm can be seen with the continuous addition of Hg 2+ . As shown in Figure , there was a good linearity ( y = 38596 × + 104328, R 2 = 0.992) between the Hg 2+ concentrations of 0 to 5 μM and the fluorescence intensity at 604 nm with a detection limit of 17 nM (3 σ /slope) . Therefore, the probe Hg‐P can detect low concentrations of Hg 2+ .…”

Section: Resultsmentioning

confidence: 76%

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

Wang

Liu

et al. 2018

Luminescence

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Currently, the fluorescent probe is an important method for detecting heavy metal ions, especially mercury ion (Hg ), which is harmful to the health of humans and the environment due to its toxicity and extensive use. In this paper, we designed and synthesized a colorimetric and long-wavelength fluorescent probe Hg-P with high sensitivity and excellent selectivity, which could detect Hg by the changes of visual color, fluorescence and absorption spectroscopy. With the addition of Hg to probe Hg-P solution, its color changed from yellow to pink, and showed a 171 nm red-shifted absorption spectrum. Probe Hg-P was used in real water and soil solution samples to detect Hg , and the result is satisfactory. Therefore, this new probe shows great value and application in detecting Hg in the environment.

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Section: Resultsmentioning

confidence: 76%

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

Wang

Liu

et al. 2018

Luminescence

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“…Additionally, the probe could quantitatively determine HOCl in the range of 0–3 n m with a detection limit of 27 pM. The probe has the capability to detect endogenous HOCl levels in RAW 264.7 macrophage cells and is also compatible with two‐photon microscope imaging . The Lin group reported a probe that displayed a fluorescence enhancement (≈16‐fold) in response to HOCl with high sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Imaging of Hypochlorous Acid by Fluorescence and Applications in Biological Systems

Yudhistira

Mulay

Kim

et al. 2019

Chemistry An Asian Journal

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In recent decades, HOCl research has attracted a lot of scientists from around the world. This chemical species is well known as an important player in the biological systems of eukaryotic organisms including humans. In the human body, HOCl is produced by the myeloperoxidase enzyme from superoxide in very low concentrations (20 to 400 μm); this species is secreted by neutrophils and monocytes to help fight pathogens. However, in the condition called “oxidative stress”, HOCl has the capability to attack many important biomolecules such as amino acids, proteins, nucleotides, nucleic acids, carbohydrates, and lipids; these reactions could ultimately contribute to a number of diseases such as neurodegenerative diseases (AD, PD, and ALS), cardiovascular diseases, and diabetes. In this review, we discuss recent efforts by scientists to synthesize various fluorophores which are attached to receptors to detect HOCl such as: chalcogen‐based oxidation, oxidation of 4‐methoxyphenol, oxime/imine, lactone ring opening, and hydrazine. These synthetic molecules, involving rational synthetic pathways, allow us to chemoselectively target HOCl and to study the level of HOCl selectivity through emission responses. Virtually all the reports here deal with well‐defined and small synthetic molecular systems. A large number of published compounds have been reported over the past years; this growing field has given scientists new insights regarding the design of the chemosensors. Reversibility, for example is considered important from the stand point of chemosensor reuse within the biological system; facile regenerability using secondary analytes to obtain the initial probe is a very promising avenue. Another aspect which is also important is the energy of the emission wavelength of the sensor; near‐infrared (NIR) emission is favorable to prevent autofluorescence and harmful irradiation of tissue; thus, extended applicability of such sensors can be made to the mouse model or animal model to help image internal organs. In this review, we describe several well‐known types of receptors that are covalently attached to the fluorophore to detect HOCl. We also discuss the common fluorophores which are used by chemist to detect HOCl, Apart from the chemical aspects, we also discuss the capabilities of the compounds to detect HOCl in living cells as measured through confocal imaging. The growing insight from HOCl probing suggests that there is still much room for improvement regarding the available molecular designs, knowledge of interplay between analytes, biological applicability, biological targeting, and chemical switching, which can also serve to further sensor and theurapeutic agent development alike.

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“…At present, various methods for detecting hemin have been found, for example, fluorometry, voltammetric determination, chemiluminescence, 12 spectrophotometric and electrochemistry . Compared to these methods, fluorometric (FL) analysis was widely investigated due to its high sensitivity, highly selectivity and simple operation . During the past few decades, many researches have focused on carbon quantum dots and CdHgSe quantum dots, which are used for fluorescence probe to detection of hemin.…”

Section: Introductionmentioning

confidence: 99%

Acid‐treated Graphitic Carbon Nitride Nanosheets as Fluorescence Probe for Detection of Hemin

Guo

Zhang

et al. 2019

ChemistrySelect

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An acid-treated, oxidized graphitic carbon nitride nanosheets (g-C 3 N 4 NSs) was successfully synthesized and applied as a fluorescence probe for the detection of hemin based on the inner filter effect (IFE). The results indicated that the fluorescence quenching of g-C 3 N 4 NSs caused by hemin was attributed to the overlap between absorption spectrum of hemin and emission spectrum of g-C 3 N 4 NSs. It is confirmed that the g-C 3 N 4 NSs exhibited a highly sensitive and selective for detection of hemin. Under the optical condition, the fluorescence changing of g-C 3 N 4 NSs is related to the concentration of hemin ranging from 0.5 -25 μM with a detection limit of 0.15 μM. Moreover, the fluorescence probe has been successfully applied to detect hemin in human blood. Overall, a novel and efficient fluorescence-based method was developed supported by g-C 3 N 4 NSs, which is a promising material for application in sensing and cell imaging.

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A fast-response, highly specific fluorescent probe for the detection of picomolar hypochlorous acid and its bioimaging applications

Cited by 69 publications

References 50 publications

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

Imaging of Hypochlorous Acid by Fluorescence and Applications in Biological Systems

Acid‐treated Graphitic Carbon Nitride Nanosheets as Fluorescence Probe for Detection of Hemin

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A fast-response, highly specific fluorescent probe for the detection of picomolar hypochlorous acid and its bioimaging applications

Cited by 69 publications

References 50 publications

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg2+

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg2+

Imaging of Hypochlorous Acid by Fluorescence and Applications in Biological Systems

Acid‐treated Graphitic Carbon Nitride Nanosheets as Fluorescence Probe for Detection of Hemin

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A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺