A Fast Protocol for in Vitro Propagation of Ginger (Zingiber Officinale Rosc.) of a Tribal District of India

In vitro propagation of ginger (Zingiber officinale Rosco)

“…However, the minimum number of leaves/shoot appeared in MC followed by M8, respectively without significant differences between them. These results are in harmony with those obtained by Jagadev et al [14] who stated that, MS medium supplemented with 3.0 mg/l BAP and 0.4 mg/l NAA was ideal for maximum number of leaves formation in Z. officinale Rosc. While, Shukla et al [29] found that MS medium supplemented with 3.0 mg/l BAP produced the optimum number of leaves of Curcuma angustifolia.…”

“…This obtained result goes in line with those findings by Salvi et al [27] who cleared that, regenerated shoots of Curcuma longa were rooted and grown on MS medium supplemented with 0.1 mg/l NAA. Moreover, Jagadev et al [14] found that for rooting of Z. officinale Rosc, MS supplemented with NAA (0.5 mg/l) was found to be more effective and produced the maximum number of roots per shoot (13.3). On other hand, Rout et al [26] indicated that the use of either IAA or IBA in the culture medium affect the root induction from shoots of Acacia chundra.…”

“…Indeed, most crop improvement programs for these species were confined to evaluation and selection of naturally occurring clonal variants. Clonal multiplication of ginger through multiple shoots induction has been reported by several workers [20,14,19]. The present study was carried out to highlight an effective, reliable and reproducible protocol for in vitro propagation of ginger.…”

“…The clone survival rate was about 95%. Also, Jagadev et al [14] reported that, acclimatization of in vitro plantlets was done on media mixture of soil:sand:farm yard manure (1:1:1) which found to be best for survival of the plantlets in ginger. However, Stanly and Lai-Keng [31] found that in vitro rooted plantlets of C. zedoaria and Zingiber zerwnbet cultured in pots containing mixture of organic soil and sand (1:1) without direct sunlight were successfully acclimatized with 100% of the plantlet survived.…”

Optimizing Photoautotrophic Micropropagation Conditions for Ginger

“…Furthermore, to improve asexual crop species, most efforts have been restricted to evaluating individuals and selecting for desired traits. However, ginger crops may be improved by clonal multiplication through the induction of multiple shoots, as has been reported by several studies (Jagadev et al 2008;Mohanty et al 2008). However, the cost of transplanting cultured seedlings to the eld accounts for 40-60% of the total costs of the tissue culture seedling production process.…”