A Fast Preparation of Skeletal Materials Using Enzyme Maceration*

Симонсен, Ким; Rasmussen, Arne Redsted; Mathisen, Pernille; Petersen, H. Ingvard; Borup, Flemming

doi:10.1111/j.1556-4029.2010.01668.x

Cited by 43 publications

(35 citation statements)

References 17 publications

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“…Every 30 min, an additional 1.34 g of fresh pancreatin powder was added and the solution was gently stirred with a wooden spoon to ensure that the enzyme was thoroughly mixed into the solution. Fresh enzyme was added to maintain the quantity of enzyme present, as it quickly denatures rendering it inactive (39). Due to the extended time required to achieve the desired results, these specimens were checked every 10 min as opposed to every 60 sec; when it became apparent that they were approaching the end stage, monitoring took place every 60 sec.…”

Section: Aqueous Methodsmentioning

confidence: 99%

Assessment of Maceration Techniques Used to Remove Soft Tissue from Bone in Cut Mark Analysis

King

Birch

2015

Journal of Forensic Sciences

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Maceration techniques employed in forensics must be effective without compromising the bone's integrity and morphology, and prevent destruction of evidence. Techniques must also be fast, safe, easily obtainable and inexpensive; not all techniques currently employed are appropriate for forensic use. To evaluate the most suitable approach, seven techniques including current and new methodologies were applied to fresh, fleshed porcine ribs exhibiting cut marks. A sample size of 30 specimens per technique was examined under scanning electron microscopy at the cut mark and the surrounding uncompromised regions; a scoring system of effectiveness was applied. The previously unpublished microwave method fared best for bone and cut mark preservation. Sodium hypochlorite destroyed cut marks, and was deemed unsuitable for forensic analysis. No single technique fulfilled all criteria; however, this study provides a benchmark for forensic anthropologists to select the most appropriate method for their situation, while maintaining the high standards required by forensic science.

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Section: Aqueous Methodsmentioning

confidence: 99%

Assessment of Maceration Techniques Used to Remove Soft Tissue from Bone in Cut Mark Analysis

King

Birch

2015

Journal of Forensic Sciences

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“…The frozen corpses were put into the cotton net and subjected to the biological maceration in the laboratory incubator at 45°C (Simonsen et al 2011). Isolated and completely cleaned skeletons were dried and preserved in the plastic cans.…”

Section: Methodsmentioning

confidence: 99%

Sexual dimorphism, asymmetry, and the effect of reproduction on pelvis bone in the bank vole, Myodes glareolus

et al. 2017

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Sexual dimorphism in anatomical traits has been widely studied in animals. Although pelvis dimorphism was mostly studied in humans, it occurs also in many other mammalian species. Here, we investigated sexual dimorphism in the pelvis of the bank vole Myodes glareolus using individuals with known sex and reproductive status of females (parous vs nulliparous). The analyses revealed that the size and shape of pelvis differed significantly between sexes, as well as between nulliparous and parous females. In comparison with males, females had a significantly longer pelvis and pubis bones and a longer obturator foramen length, but a smaller pubis width. Interestingly, the difference between parous and nulliparous females resembles that between females and males: parous females had bigger pelvis, which probably resulted from changes during pregnancy and after birth. Left bones were on average larger than right ones, but the magnitude of directional asymmetry was not different between sex and reproduction group. Moreover, we noticed that fluctuating asymmetry of pelvis and pubis length was higher in females than in males and higher in parous than in nulliparous females, what is presumably associated with locomotor performance. A discriminant function analysis performed for the four bone size traits showed that the traits can be effectively used for a nearly perfect recognition of sexes and also a quite reliable recognition of the reproductive status of females.

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“…Enzymatic maceration has been declared to be the fastest and bones obtained are quite clean, but the problem is the obnoxious smell that develops during the process and high cost of enzymes used [7].…”

Section: Discussionmentioning

confidence: 99%

“…Different methods of bone cleaning like boiling [5], manual cleaning by scraping, maceration with water or use of detergents [6] have been tried by many. Use of various chemicals like enzymes [7], hydroxides [8] and even of bugs [9] are practiced and available in the text, but they seem difficult, time consuming and may need some special kind of place or equipment. These procedures might also need experienced manpower.…”

Section: Introductionmentioning

confidence: 99%

An Alternative Approach to Bone Cleaning Methods for Anatomical Purposes

Aggarwal¹,

Gupta²,

Goyal³

et al. 2016

IJAR

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Introduction: Many methods of bone cleaning have been mentioned and followed by various experts over period of years. Most of them are tedious and time consuming. Also they need training and expertise of persons doing these procedures. In this study we have tried to modify some of the older methods to make the extraction and cleaning of bones easy and less time consuming. Also we expect that the bones extracted by this method will be more presentable for study. Thus the preparation of bones specimens by this method can be of immense value and time saver for many research institutes to get their self prepared bone specimens and thus help them in becoming self sufficient. Materials and Methods:Lots of the donated bodies under the ongoing body donation programme are being used in the department of Anatomy, AIMSR. After dissection and autopsy in the dissection hall, the cadavers which have been buried for a period of about 1 year were dug for the remains. The bones were collected with the help of technicians and washed thoroughly with tap water. The bones were then put in varying concentration of H 2 O 2 and observed for desired changes.It was also recorded that how much time did each concentration take to clean the bone of its soft tissue. Also the amount of bleaching/whitening of bone will be noticed. Care was taken that none of the original features of bone were lost or attenuated. If at lower concentration some of the tissue was not cleaned off the bones, then higher concentration of hydrogen peroxide were tried. Conclusion:Finally we were able to conclude that at what concentration the bones clean easily and at earliest without damage and with required amount of bleaching. They were then dipped in solution of fixative for preventing damage and giving them a presentable look.

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A Fast Preparation of Skeletal Materials Using Enzyme Maceration*

Cited by 43 publications

References 17 publications

Assessment of Maceration Techniques Used to Remove Soft Tissue from Bone in Cut Mark Analysis

Assessment of Maceration Techniques Used to Remove Soft Tissue from Bone in Cut Mark Analysis

Sexual dimorphism, asymmetry, and the effect of reproduction on pelvis bone in the bank vole, Myodes glareolus

An Alternative Approach to Bone Cleaning Methods for Anatomical Purposes

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