A fast, precise, in‐vivo method for micron‐level <scp>3D</scp> models of corals using dental scanners

Quigley, Kate M.

doi:10.1111/2041-210x.13959

Cited by 4 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the 2D size of small organisms (≤1–1.5 mm) was measured as the points making up the cloud were either not dense enough or had little holes reducing confidence to truly represent their volume at such small size (Figure S8). Using this study workflow, we recommend measuring volume of organisms only when >3–5 mm in size (Figure S8), which is larger than juvenile corals quantified in Quigley (2022) using a dental scanners in ex situ laboratory settings.…”

Section: Discussionmentioning

confidence: 99%

Underwater macrophotogrammetry to monitor in situ benthic communities at submillimetre scale

et al. 2023

View full text Add to dashboard Cite

Larval settlement and recruitment of sessile organisms are key ecological processes for population recovery and maintenance that occur at scales invisible to the human eye. Accordingly, proxies of recruitment have commonly been quantified using artificial substrata such as settlement tiles made of diverse materials and shapes, which are typically transported to the laboratory for examination. However, it is unknown how much bias is introduced with this sampling strategy and how recruitment quantified on tiles relates to recruitment on nearby natural substrata. Here, we applied techniques that combine macrophotography with photogrammetry (macrophotogrammetry) underwater to monitor benthic communities at submillimetre scale. This application allows the investigation of recruitment and community succession of the earliest life‐history stages in situ and on natural substrata. We tested the use of four different imaging systems, varying in costs from US$ 1400 to US$ 5440. While the most expensive SONY αRiv system provided the best visual output and ground resolution (up to 5 μm/pixel with a + 4 close‐up lens); regardless of systems, 3D models always had a ground resolution ≤23 μm/pixel and errors in planar measurements of submillimetre features were similar among systems. This level of resolution compares well with stereomicroscopy in the range of 5:1 to 10:1 magnification, while providing detailed 3D digital records through time. Using a coral reef example, we apply this approach to demonstrate how it can be used to monitor small reef areas (~300–600 cm2) through time, including the quantification of biophysical metrics such as cover of small facilitative and competitive organisms and microhabitat complexity. We further show that organisms as small 0.5 mm in size, such as 2‐month‐old coral settlers, can be located accurately within the 3D models and measured with a good level of confidence. This method can be readily applied to other benthic environments to elucidate drivers of early recruitment and recovery of benthic organisms following disturbance impacts at very fine scales, directly on natural substrata, to avoid biases inherent with laboratory‐based analyses of artificial surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

Underwater macrophotogrammetry to monitor in situ benthic communities at submillimetre scale

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Respiration is a critical biological measurement that signals the overall metabolic activity of an organism, but like other critical traits (growth), is hard to measure in small organisms 1 .…”

Section: Introductionmentioning

confidence: 99%

Physiological Characterization of the Coral Holobiont Using a New Micro-Respirometry Tool

Quigley¹,

Carey

Roa

2023

JoVE

View full text Add to dashboard Cite

Metabolic activity, defined as the sum of organismal processes that involve energy, is of critical importance in understanding the function and evolution of life on earth.Measuring organismal metabolic rates is, therefore, at the center of explaining the physiological states of organisms, their ecological roles, and the impact of environmental change on species within terrestrial and aquatic ecosystems. On coral reefs, measures of metabolism have been used to quantify symbiosis functioning between corals and their obligate algal symbionts (Symbiodiniaceae), as well as assess how environmental stressors, including climate change, will impact coral health. Despite this significance, there is a lack of methods, and therefore data, relating to metabolic rate measurements in coral offspring, likely due to their small size. To address this gap, this study aimed to develop a custom setup for measuring the respiration of small (millimeter size range) marine animal ecologies. This low cost and easy setup should allow for the improved measurement of metabolic rate. This will be essential for applied ecological research utilizing the sexual production of corals for reef restoration.

show abstract

3D scanning measurement method for seed size research

Málik-Roffa,

Málik,

Tőzsér

et al. 2024

Preprint

View full text Add to dashboard Cite

Premise: Traditionally used seed size and surface area calculation techniques are often inaccurate or destructive. This is especially relevant for organisms with irregular shapes. It is believed that three-dimensional (3D) printing and scanning could also be disruptive technologies. However, these methods hold immense potential for ecology and evolution sciences, which have a longstanding tradition of inventing and creating objects for research, education, and outreach. Methods and Results: We describe how to 3D scan seeds reproducible on an inexpensive structured light based commodity 3D scanner. We then compared novel 3D and traditional 2D approaches by reproducibility and timing. Conclusions: This study demonstrates how 3D scanning methods are easily applicable to the quantification of seed shape and providing an easy entry into it.

show abstract

A fast, precise, in‐vivo method for micron‐level 3D models of corals using dental scanners

Cited by 4 publications

References 36 publications

Underwater macrophotogrammetry to monitor in situ benthic communities at submillimetre scale

Underwater macrophotogrammetry to monitor in situ benthic communities at submillimetre scale

Physiological Characterization of the Coral Holobiont Using a New Micro-Respirometry Tool

3D scanning measurement method for seed size research

Contact Info

Product

Resources

About