A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate

Zappacosta, Bruno; Persichilli, Silvia; Mormile, F.; Minucci, Angelo; Russo, A.; Giardina, Bruno; Sole, P. De

doi:10.1016/s0009-8981(01)00571-x

Cited by 47 publications

(28 citation statements)

References 21 publications

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“…These substances are able to reach the exhaled breath and can easily be detected in EBC. The detectable concentration of H 2 O 2 in the present male smokers was within the concentration range of all previous observations in healthy smokers (0.10-0.75 mM) [5,6,18]. This wide range of exhaled H 2 O 2 concentration may be explained, in part, by the variety of condensing devices of different design that were used for the collection of EBC samples or by differences in the analytical methods used for measurement of H 2 O 2 .…”

Section: Discussionsupporting

confidence: 78%

Quantitative analysis of 8‐isoprostane and hydrogen peroxide in exhaled breath condensate: Table 1—

Hoydonck¹,

Wuyts²,

Vanaudenaerde³

et al. 2003

Eur Respir J

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Exhaled breath condensate (EBC) provides a noninvasive means of sampling the lower respiratory tract. Collection of EBC might be useful in the assessment of airway oxidative stress in smokers. The aim of this study was to determine 8-isoprostane and hydrogen peroxide levels in EBC, and, in addition, to investigate the reproducibility of these measurements.EBC samples were collected from 12 healthy male smokers at three time points within 1 week. 8-isoprostane and H 2 O 2 were measured in nonconcentrated EBC using immunochemical and colorimetric assays, respectively. 8-isoprostane and H 2 O 2 were detected in only 36 and 47% of all EBC samples, respectively. It was not possible to calculate the within-subject variation in a reliable manner since only three of the 12 smokers exhibited detectable 8-isoprostane concentrations on all three occasions (mean 4.6 pg?mL -1 ; range 3.9-7.7 pg?mL -1 ), whereas H 2 O 2 could not be detected on all three occasions in any of the smokers. Spiking experiments revealed a recovery of 83.5-109.5% for 8-isoprostane and 69.9-129.0% for H 2 O 2 in fresh EBC samples.It was concluded that levels of 8-isoprostane and hydrogen peroxide cannot be reproducibly assessed in exhaled breath condensate from healthy smokers because of their low concentration and/or the lack of sensitivity of the available assays.

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Section: Discussionsupporting

confidence: 78%

Quantitative analysis of 8‐isoprostane and hydrogen peroxide in exhaled breath condensate: Table 1—

Hoydonck¹,

Wuyts²,

Vanaudenaerde³

et al. 2003

Eur Respir J

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“…Therefore, the use of more sensitive methods is advisable for peroxide measurement and further studies are required to establish the usefulness of other methods (i.e. chemiluminescence or other) for H 2 O 2 measurements in EBC [59,[67][68][69][70].…”

Section: Measurements Of Mediators In Ebcmentioning

confidence: 99%

Exhaled breath condensate: methodological recommendations and unresolved questions

Ho¹,

Hunt²,

Barnes³

2005

Eur Respir J

1,159

1,315

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Collection of exhaled breath condensate (EBC) is a noninvasive method for obtaining samples from the lungs. EBC contains large number of mediators including adenosine, ammonia, hydrogen peroxide, isoprostanes, leukotrienes, nitrogen oxides, peptides and cytokines. Concentrations of these mediators are influenced by lung diseases and modulated by therapeutic interventions. Similarly EBC pH also changes in respiratory diseases.The aim of the American Thoracic Society/European Respiratory Society Task Force on EBC was to identify the important methodological issues surrounding EBC collection and assay, to provide recommendations for the measurements and to highlight areas where further research is required.Based on the currently available evidence and the consensus of the expert panel for EBC collection, the following general recommendations were put together for oral sample collection: collect during tidal breathing using a noseclip and a saliva trap; define cooling temperature and collection time (10 min is generally sufficient to obtain 1-2 mL of sample and well tolerated by patients); use inert material for condenser; do not use resistor and do not use filter between the subject and the condenser. These are only general recommendations and certain circumstances may dictate variation from them.Important areas for future research involve: ascertaining mechanisms and site of exhaled breath condensate particle formation; determination of dilution markers; improving reproducibility; employment of EBC in longitudinal studies; and determining the utility of exhaled breath condensate measures for the management of individual patients. These studies are required before recommending this technique for use in clinical practice.

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“…[6][7][8] The development of methods for the determination of these substances is significant in the fields of clinical and biological studies. Several methods for the determination of the spectrophotometry, [9][10][11] fluorophotometry, [12][13][14][15][16][17][18] and chemiluminescence 19,20) of H 2 O 2 and other ROS have already been reported. These methods focus on the determination of individual ROS.…”

mentioning

confidence: 99%

Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

et al. 2008

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977Reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ), superoxide (O 2 Ϫ ), hydroxyl radical ( · OH), nitric oxide (NO), peroxynitrite (ONOO Ϫ ), and peroxide (R-OOH) affect the living body and cause various types of diseases such as cancer, 1) cardiovascular disorders, 2) and neurodegenerative diseases. 3,4) On the other hand, it is accepted that some ROS control disinfection in cells and work as a biofactor for signal transmission of insulin. 5) In addition, H 2 O 2 is a major by-product of ROS in living organisms and a common marker for oxidative stress in the field of clinical inspection.6-8) The development of methods for the determination of these substances is significant in the fields of clinical and biological studies. Several methods for the determination of the spectrophotometry, 9-11) fluorophotometry, [12][13][14][15][16][17][18] and chemiluminescence 19,20) of H 2 O 2 and other ROS have already been reported. These methods focus on the determination of individual ROS. However, it appears that most of ROS have only minute life times under biological environments. Therefore, we considered that it is more practicable to measure the total rather than individual ROS for a clinical purpose. Hence, we developed a handy and highly sensitivity method for the determination of ROS, including H 2 O 2 . We have already developed methods for the fluorometric determination of cobalt(II) and H 2 O 2 with fluorescein hydrazide (FH), 21) however, the method adopted for the determination of H 2 O 2 exhibited remarkably poor reproducibility. In this study, method to obtain a more reproducible and sensitive spectrofluorimetric determination of H 2 O 2 and other ROS were examined. Furthermore, the applicability of the method for measuring H 2 O 2 in urine samples was examined. Moreover, because an excellent crystal of FH was obtained, structural analysis by X-ray diffractometry was performed. As a result, this FH crystal was confirmed to have a five-membered spirolactam structure. The relationship between fluorescence and the chemical structure was studied; the fluorescein, which was the strong fluorescent substance that caused the spirolactam formation by acting as the hydrazine conductor, became a colorless, non-fluorescent substance. Experimental Synthesis of Fluorescein Hydrazide (FH)A modification of the procedure of Baeyer and Akita. 22,23) for the synthesis of fluoescein and FH was employed. Resorcinol 2 mol and phthalic anhydride 1 mol were combined with methanesulfonic acid. The mixture was heated and dissolved in 5% sodium hydroxide solution. The solution was poured into 30% acetic acid; the resulting fluorescein, a yellow precipitate, was collected with a yield of 92.5%. Subsequently, fluorescein was added to a solution of excess 98% anhydrous hydrazine and heated at 80°C in a warm water bath for 3 h. Upon cooling to room temperature and with the addition of strong hydrochloric acid into the solution, the light yellow substance was deposited. Then, the substance obtained was dissolved in ethan...

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A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate

Cited by 47 publications

References 21 publications

Quantitative analysis of 8‐isoprostane and hydrogen peroxide in exhaled breath condensate: Table 1—

Quantitative analysis of 8‐isoprostane and hydrogen peroxide in exhaled breath condensate: Table 1—

Exhaled breath condensate: methodological recommendations and unresolved questions

Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

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