A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures

Kolmert, Åsa; Wikström, Pernilla; Hallberg, Kevin B.

doi:10.1016/s0167-7012(00)00154-8

Cited by 224 publications

(123 citation statements)

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“…The exact sulfide content of standards was determined by iodometric titration (Vogel, 1961). Sulfate analyses were performed by a turbidimetric method (Kolmert et al, 2000) after removing sulfide with zinc carbonate. Acetate concentration was determined by HPLC (Waters 600E equipped with a Phenomenex Rezex organic acids column) after removing sulfide with zinc carbonate.…”

mentioning

confidence: 99%

Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium

Cravo‐Laureau

Labat

Joulian

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A novel anaerobic, long-chain alkene-degrading, sulfate-reducing bacterium, strain LM2801T, was isolated from brackish sediment of a wastewater decantation facility of an oil refinery (Berre lagoon, France). Cells of strain LM2801T were Gram-negative, motile, slightly curved or vibrioid rods. Its optimum growth conditions were 30–36 °C, 6–10 g NaCl l−1 and pH 7.5. Strain LM2801T incompletely oxidized long-chain alkenes (from C14 to C23) and fatty acids (C14 to C24). The DNA G+C content was 45.5 mol%. Sequence analyses of the 16S rRNA and dsrAB genes indicated that the strain was a member of the family Desulfobacteraceae within the Deltaproteobacteria. This novel isolate possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus. Therefore, strain LM2801T is described as a member of a new genus, Desulfatiferula gen. nov., of which Desulfatiferula olefinivorans sp. nov. is the type species. The type strain of Desulfatiferula olefinivorans is LM2801T (=DSM 18843T =JCM 14469T).

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mentioning

confidence: 99%

Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium

Cravo‐Laureau

Labat

Joulian

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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“…Vários trabalhos já demonstraram a importância do sulfato, em sítios contaminados com compostos orgânicos aromáticos, uma vez que este aceptor final de elétrons possibilita o desenvolvimento de bactérias redutoras de sulfato (BRS) (Boopathy et al, 1998;Kolmert et al, 2000;Franzmann et al, 2002;Da Silva et al, 2005). Confirmando a tendência dos dados obtidos in situ, pelos autores anteriormente citados, o biofilme sulfetogênico formado no RAHLF também se mostrou capaz de remover tolueno (Cattony et al, 2005).…”

Section: Resultsunclassified

Remoção de etanol e benzeno em reator anaeróbio horizontal de leito fixo na presença de sulfato

Cattony

Ribeiro

Zaiat

et al. 2007

Eng. Sanit. Ambient.

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Reator anaeróbio horizontal de leito fixo (RAHLF), preenchido com espumas de poliuretano, foi usado para tratar benzeno em solução etanólica, sob condições sulfetogênicas. Benzeno foi adicionado em concentração inicial de 2,0 mg.l-1, seguido de aumentos que variaram até 10 mg.l-1. O etanol foi adicionado em concentrações de 170 mg.l-1 a 980 mg.l-1. Soluções de sulfato ferroso e sulfato de sódio foram usadas, nas concentrações de 91 e 550 mg.l-1, respectivamente. O reator foi operado a 30 (± 2) ºC com tempo de detenção hidráulica de 12 h. A remoção da matéria orgânica foi próxima a 90% com taxa máxima de degradação de benzeno de 0,07 mg benzeno.mg-1SSV.d-1. O presente trabalho corrobora os dados obtidos por Cattony et al (2005), na medida em que torna mais consistente a proposta do uso de unidades compactas de RAHLF, para a biorremediação in situ de compostos aromáticos.

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“…Ferrous and total iron were quantified as described before Ecological niches of acidophilic microorganisms S Ziegler et al (Stookey, 1970;Ziegler et al, 2009). Sulfate quantification was conducted as described by Kolmert et al (2000).…”

Section: Determination Of Elemental Compositionsmentioning

confidence: 99%

“…The concentration of dissolved Na is 1.6-fold higher at the anoxic center compared to the oxic area, while the K concentration in the anoxic part is only 0.7-fold of what was detected at the oxic part (Table 1). Considering the amount of dissolved sulfur per liter (92-97 mM) as quantified by ICP-OES, it becomes evident that sulfate is the major contributor of the detected dissolved sulfur as the conducted sulfate quantifications from a whole biofilm via the method from Kolmert et al (2000) reveal concentrations of 95 mM (±3.8 mM). The quantification of dissolved iron (47-50 mM) by ICP is in agreement with the quantifications of dissolved ferric (47.4±4.0 mM) and ferrous iron (8.3±1.5 mM) by the ferrozine assay (Stookey, 1970).…”

Section: Characterization Of the Possible Niches In The Biofilmmentioning

confidence: 99%

Oxygen-dependent niche formation of a pyrite-dependent acidophilic consortium built by archaea and bacteria

et al. 2013

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Biofilms can provide a number of different ecological niches for microorganisms. Here, a multispecies biofilm was studied in which pyrite-oxidizing microbes are the primary producers. Its stability allowed not only detailed fluorescence in situ hybridization (FISH)-based characterization of the microbial population in different areas of the biofilm but also to integrate these results with oxygen and pH microsensor measurements conducted before. The O 2 concentration declined rapidly from the outside to the inside of the biofilm. Hence, part of the population lives under microoxic or anoxic conditions. Leptospirillum ferrooxidans strains dominate the microbial population but are only located in the oxic periphery of the snottite structure. Interestingly, archaea were identified only in the anoxic parts of the biofilm. The archaeal community consists mainly of so far uncultured Thermoplasmatales as well as novel ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganism) species. Inductively coupled plasma analysis and X-ray absorption near edge structure spectra provide further insight in the biofilm characteristics but revealed no other major factors than oxygen affecting the distribution of bacteria and archaea. In addition to catalyzed reporter deposition FISH and oxygen microsensor measurements, microautoradiographic FISH was used to identify areas in which active CO 2 fixation takes place. Leptospirilla as well as acidithiobacilli were identified as primary producers. Fixation of gaseous CO 2 seems to proceed only in the outer rim of the snottite. Archaea inhabiting the snottite core do not seem to contribute to the primary production. This work gives insight in the ecological niches of acidophilic microorganisms and their role in a consortium. The data provided the basis for the enrichment of uncultured archaea.

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A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures

Cited by 224 publications

References 10 publications

Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium

Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium

Remoção de etanol e benzeno em reator anaeróbio horizontal de leito fixo na presença de sulfato

Oxygen-dependent niche formation of a pyrite-dependent acidophilic consortium built by archaea and bacteria

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