A fast and simple FIA-chemiluminescence method for the evaluation of Roselle flowers as scavenger of the free radicals generated by UV irradiated antibiotics

Pulgarı́n, José A. Murillo; Bermejo, Luisa F. García; Durán, Armando Carrasquero

doi:10.1016/j.jpba.2018.11.004

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…The ability of roselle to remove ROS has been reflected in animal experiments and confirmed by a reduction in luminol chemiluminescence intensity. It has been demonstrated that roselle can eliminate free radicals generated through radiation [9].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Lee,

Huang,

et al. 2024

Fishes

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(1) Background: Roselle (Hibiscus sabdariffa) is a flowering plant reported to have anti-obesity, antioxidant, antibacterial, and anti-inflammatory effects. This study aims to evaluate the UV-absorbing and antioxidant activities of roselle aqueous extracts (RAE) and test the protective effects of RAE against UV radiation in zebrafish embryos. (2) Methods: DPPH assay and UV-spectrum methods were applied to evaluate the antioxidant and UV-absorbing activities, respectively. The protective effects of RAE were evaluated using fin morphology recording, Kaplan–Meier analysis, and Cox proportional hazards regression. Real-time PCR experiments were also applied to detect both the UV- and RAE-induced gene expressions. (3) Results: Our results show that (i) RAE had UV-absorbing abilities and significantly reduced ROS production in vitro; (ii) the mean times of malformed fins in the UV + RAE (36 and 48 ppm) groups were 3.56 and 4.44 days, respectively, and were prolonged compared to those in the UV-only group (3.36 days); (iii) zebrafish in the UV + RAE (36 and 48 ppm) groups were 0.963 and 0.496 (p < 0.001) times more likely to develop to malformed fins, respectively, than those in the UV-only group; and (iv) the RAE treatment led to the 0.19- to 0.62-fold downregulation of the p53, p21, mdm2, and bcl2 gene expressions, compared to the UV-only group. (4) Conclusions: The UV-protective effects of RAE might derive from both the in vitro UV-absorbing activity and in vivo regulation of the p53, p21, mdm2, and bcl2 gene expressions.

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Section: Introductionmentioning

confidence: 99%

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Lee,

Huang,

et al. 2024

Fishes

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“…Collagen can reduce skin wrinkles and maintain immunity from infection and allergies. These anthocyanins and vitamin C are essential in warding off free radicals that can cause degenerative diseases [11]. Vitamin C, or ascorbic acid, is an organic compound that the body cannot synthesize.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasound Assisted Extraction (UAE) Conditions on Vitamin C from Roselle Flower

Rosalinda,

Fitriyani,

Mardawati

2024

Int. J. Adv. Sci. Eng. Inf. Technol.

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Roselle plant (Hibiscus sabdariffa L.) has many benefits. Vitamin C is an antioxidant in which the human body required. It contains a high amount of vitamin C. However, vitamin C is susceptible to high temperatures. Proper extraction treatment conditions produce optimum vitamin C. This study aims to determine the optimization of vitamin C in rosella flowers. This research uses UAE with Qsonica type – Q500 (500 W, 20 kHz). Extraction treatment conditions control amplitude, time and amount of solvent. The research method used is a laboratory experiment with the RSM (Response Surface Methodology) type CCD (Central Composite Design) approach. The research method was an experimental laboratory using a CCD (Central Composite Design) via RSM (Response Surface Methodology) with an amplitude level from 20% to 50%, a time level from 10 minutes to 20 minutes, and a ratio of the amount of solvent from 140 mL to 260 mL by weight. The results showed that the length of time and the amount of solvent had a significant effect, while the amplitude had no significant effect. The significant quadratic model at p value 0.05 and R2 0.9221 with optimum UAE conditions in the treatment combination of 50 percent amplitude, 20 minutes of time, and 140 ml of solvent resulted in an optimum vitamin C content of 38.76 mg/100g. This research can be used as a reference to determine the value of vitamin C with different amplitude, time, and amount of solvent from the research data.

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One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine

Mei

Liu

Xiong

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

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A fast and simple FIA-chemiluminescence method for the evaluation of Roselle flowers as scavenger of the free radicals generated by UV irradiated antibiotics

Cited by 6 publications

References 18 publications

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Optimization of Ultrasound Assisted Extraction (UAE) Conditions on Vitamin C from Roselle Flower

One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine

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