A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism

Nowroozi-Nejad, Zohreh; Bahramian, Bahram; Hosseinkhani, Saman

doi:10.1007/s11164-019-03748-w

Cited by 15 publications

(19 citation statements)

References 38 publications

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“…At pH 10.0, different kinetic behavior of ZIF-8@Luc was observed including a significant BL signal decrease (∼95% at 200 s). It has been reported that enzymes attached to positively charged supports, such as ZIF-8 at pH 7–8, present stronger activity at lower pH values. , On the other hand, when the heating process was carried out up to 50 °C, the BL signal of ZIF-8@Luc was reduced (80% signal loss) more than that of the free luciferase signal (40% signal loss), despite the thermal stability of ZIF-8. This could be due to the agglomeration of ZIF-8 occurring when the biocomposite is heated, thus leading to a decrease in the available catalytic sites of luciferase.…”

Section: Resultsmentioning

confidence: 99%

“…Synthesis of ZIF-8@Luc and Method Optimization. The synthesis of the biocomposite was accomplished by taking the method described by Nowroozi-Nejad et al 31 as the starting point with several modifications and parameter optimization. The following conditions were studied in detail: type of stirring (magnetic, orbital, vortex-assisted), MOF nature (MIL-n, UiO-n, ZIF-n), amount of MOF (0.25−0.75 mg), and reaction time (15−60 min).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Novel Nanozeolitic Imidazolate Framework (ZIF-8)–Luciferase Biocomposite for Nanosensing Applications

Martínez-Pérez-Cejuela

Gregucci

Calabretta

et al. 2022

Anal. Chem.

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The identification of new strategies to improve the stability of proteins is of utmost importance for a number of applications, from biosensing to biocatalysis. Metal−organic frameworks (MOFs) have been shown as a versatile host platform for the immobilization of proteins, with the potential to protect proteins in harsh conditions. In this work, a new thermostable luciferase mutant has been selected as a bioluminescent protein model to investigate the suitability of MOFs to improve its stability and prompt its applications in real-world applications, for example, ATP detection in portable systems. The luciferase has been immobilized onto zeolitic imidazolate framework-8 (ZIF-8) to obtain a bioluminescent biocomposite with enhanced performance. The biocomposite ZIF-8@luc has been characterized in harsh conditions (e.g., high temperature, non-native pH, etc.). Bioluminescence properties confirmed that MOF enhanced the luciferase stability at acidic pH, in the presence of organic solvents, and at −20 °C. To assess the feasibility of this approach, the recyclability, storage stability, precision, and Michaelis−Menten constants (K m ) for ATP and D-luciferin have been also evaluated. As a proof of principle, the suitability for ATP detection was investigated and the biocomposite outperformed the free enzyme in the same experimental conditions, achieving a limit of detection for ATP down to 0.2 fmol.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Novel Nanozeolitic Imidazolate Framework (ZIF-8)–Luciferase Biocomposite for Nanosensing Applications

Martínez-Pérez-Cejuela

Gregucci

Calabretta

et al. 2022

Anal. Chem.

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“…In this study, the validity of this in situ one-step methodology for enzyme immobilization onto NH 2 -MIL-53(Al) is extended to lipase CaLB (from Candida antarctica), which indeed is one of the most widely investigated enzymes in different immobilization processes [34][35][36]. Apart from our own studies using nanocrystalline NH 2 -MIL-53(Al) supports for enzymes [20,21], the MIL-53(Al) family has proved to be efficient in the immobilization of: (i) Laccase on NH 2 -MIL-53(Al), using our one-step room-temperature methodology [37]; (ii) laccase on meso-MIL-53(Al) by post-synthesis methods [38]; and (iii) luciferase on non-functionalized MIL-53(Al), also by post-synthesis methodology [39]. We also found excellent results for lipase in situ immobilization on the semi-crystalline support Fe-BTC [23], suggesting that this enzyme could be easily immobilized onto MOF supports.…”

Section: Introductionmentioning

confidence: 99%

Efficient One-Step Immobilization of CaLB Lipase over MOF Support NH2-MIL-53(Al)

et al. 2020

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Metal-organic framework (MOF) materials possess the widest versatility in structure, composition, and synthesis procedures amongst the known families of materials. On the other hand, the extraordinary affinity between MOFs and enzymes has led to widely investigating these materials as platforms to support these catalytic proteins in recent years. In this work, the MOF material NH2-MIL-53(Al) has been tested as a support to immobilize by one-step methodology (in situ) the enzyme lipase CaLB from Candida antarctica by employing conditions that are compatible with its enzymatic activity (room temperature, aqueous solution, and moderate pH values). Once the nature of the linker deprotonating agent or the synthesis time were optimized, the MOF material resulted in quite efficient entrapping of the lipase CaLB through this in situ approach (>85% of the present enzyme in the synthesis media) while the supported enzyme retained acceptable activity (29% compared to the free enzyme) and had scarce enzyme leaching. The equivalent post-synthetic method led to biocatalysts with lower enzyme loading values. These results make clear that the formation of MOF support in the presence of the enzyme to be immobilized substantially improves the efficiency of the biocatalysts support for retaining the enzyme and limits their leaching.

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“…In addition, the molecules of solvent trapped in the MOF pores reduce the specific surface area. On the other hand, the Al-MOF powders have been agglomerated and the gas penetration does not occur completely ( Rahmani and Rahmani, 2018 ; Nowroozi-Nejad et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we have chosen one type of Al-MOFs as a carrier for the anti-proliferative herbal extract. The Al-MOFs seem to be more effective against the proliferation of breast cancer cells when compared with herbal extraction ( Nowroozi-Nejad et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Al-Based Metal-Organic Framework in Water With Caffeic Acid Ligand and NaOH as Linker Sources With Highly Efficient Anticancer Treatment

et al. 2021

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In this study, novel nanostructures of aluminum base metal-organic framework (Al-MOF) samples were synthesized using a sustainable, non-toxic, and cost-effective green synthesis route. Satureja hortensis extract was used as an effective source of linker for the development of the Al-MOF structures. The Fourier-transformed infrared (FTIR) spectrum confirmed the presence of characterization bonds related to the Al-MOF nanostructures synthesized by the green synthesis route. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses revealed that the sample synthesized by Na2-CA was composed of multilayers, although it was agglomerated, but it had dispersed and occurred in spherical particles, indicating active organic matter. N2 adsorption/desorption isotherms demonstrated the significant porosity of the Al-MOF samples that facilitate the high potential of these nanostructures in medical applications. The anticancer treatment of Al-MOF samples was performed with different concentrations using the MTT standard method with untreated cancer cells for 24 and 48 h periods. The results exhibited the significant anticancer properties of Al-MOF samples developed in this study when compared with other MOF samples. Thus, the development of a novel Al-MOF and its application as a natural linker can influence the anticancer treatment of the samples. According to the results, the products developed in this study can be used in more applications such as biosensors, catalysts, and novel adsorbents.

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A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism

Cited by 15 publications

References 38 publications

Novel Nanozeolitic Imidazolate Framework (ZIF-8)–Luciferase Biocomposite for Nanosensing Applications

Novel Nanozeolitic Imidazolate Framework (ZIF-8)–Luciferase Biocomposite for Nanosensing Applications

Efficient One-Step Immobilization of CaLB Lipase over MOF Support NH2-MIL-53(Al)

Synthesis of Al-Based Metal-Organic Framework in Water With Caffeic Acid Ligand and NaOH as Linker Sources With Highly Efficient Anticancer Treatment

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