A fast and efficient HPLC-PDA–MS method for detection and identification of pyranochromanone acids in Calophyllum species

Piccinelli, Anna Lisa; Kabani, Ahmad Oubada; Lotti, Cinzia; Alarcón, Adonis Bello; Cuesta‐Rubio, Osmany; Rastrelli, Luca

doi:10.1016/j.jpba.2012.12.028

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Apetalic acid, isoapetalic acid, calolongic acid, pinetoric acid I and pinetoric acid II ( Figure 1) were detected in both extracts by HPLC analysis as previously reported [(Piccinelli et al, 2013)]. In brief, MeOH/H 2 O 8:2 (v/v) solution of each extract (3 mg mL −1 ) was prepared.…”

Section: Detection Of Pyranochromanone Acids By Hplc-pda-esi-ms Analysissupporting

confidence: 63%

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Oubada

García

Bello

et al. 2014

Emir. J. Food Agric

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Calophyllum rivulare is an endemic tree from Cuba that belongs to Clusiaceae family. This plant had revealed it to be a rich source of pyranochromanone acids including compounds with a variety of biological potentialities such as antioxidant, antiulcer, anticancer and anti-Helicobacter pylori activities. Herein, antileishmanial and cytotoxic activities of extracts obtained from C. rivulare leaves (rich in pyranochromanone acids) and amentoflavone were compared. Extracts were prepared by maceration employing ethyl acetate (CR-1) and methanol (CR-2) as solvents and amentoflavone was isolated from both extracts. The CR-2 extract showed the better activity against the parasite, with an IC 50 value of 95.1 ± 5.4 µg/mL and 20.6 ± 1.4 µg/mL against promastigote and amastigote forms, respectively. This extract showed lower cytotoxicity (IC 50 =182.4 ± 2.3 µg/mL) against peritoneal macrophage from BALB/c mice and a selectivity index of 9. Amentoflavone showed an IC50 of 5.9 ± 0.3 µg/mL against amastigote form and a selectivity index of 9. Results suggest that CR-2 extract and amentoflavone could be explored as new antileishmanial alternatives.

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Section: Detection Of Pyranochromanone Acids By Hplc-pda-esi-ms Analysissupporting

confidence: 63%

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Oubada

García

Bello

et al. 2014

Emir. J. Food Agric

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“…pinetorum , and C . rivulare growing in Cuba were the same in all these species (Piccinelli et al ., ), but different with respect to main chemical compounds reported for C . inophyllum from other geographical origins (Patil et al ., ).…”

Section: Microorganisms and Cellsmentioning

confidence: 75%

“…The mass analyses were performed with an enclosure services interface in the positive modes using a LCQ Advantage ion‐trap mass spectrometer (ThermoFinningan, San Jose, CA, USA). The data were acquired in the full scan (range of m / z 200–1000) and dependent scan modes, the maximum injection time was 50 ms. All chromatograms were compared with those obtained in a previous study in order to confirm the identity of each peak and then the presence of pyranochromanones (Piccinelli et al ., ).…”

Section: Microorganisms and Cellsmentioning

confidence: 97%

“…Our results are in concordance with previous studies, where resins of three different Calophyllum spp from Cuba, including C . antillanum , contain apetalic acid, isoapetalic acid, calolongic acid, pinetoric acid I, pinetoric acid II, 2,3‐cis calolongic acid, pinetoric acid III, and isopinetoric acid II (Piccinelli et al ., ). The chemical analysis indicated that resins of C .…”

Section: Microorganisms and Cellsmentioning

confidence: 97%

“…The chemical analysis indicated that resins of C . antillanum , Calophyllum pinetorum , and Calophyllum rivulare were very similar from a chemical point of view, and their main constituents were recognized as pyranochromanones (Piccinelli et al ., ). It is very interesting to note that the chemical components detected in C .…”

Section: Microorganisms and Cellsmentioning

confidence: 97%

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Antimicrobial Assessment of Resins fromCalophyllum AntillanumandCalophyllum Inophyllum

et al. 2015

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The Calophyllum genus is well-known for its antimicrobial and cytotoxic activities, and therefore, we analyzed these biological activities for resins of Calophyllum antillanum and Calophyllum inophyllum growing in Cuba. C. antillanum resins showed a potent activity against Plasmodium falciparum (IC50 = 0.3 ± 0.1 µg/mL), while its cytotoxicity against MRC-5 cells was much lower (IC50 = 21.6 ± 1.1 µg/mL). In contrary, the resin of C. inophyllum showed an unspecific activity. The presence of apetalic acid, isoapetalic acid, calolongic acid, pinetoric acid I, pinetoric acid II, isocalolongic acid, pinetoric acid III, and isopinetoric acid III in C. antillanum resins was also confirmed. These results demonstrated for the first time the potential activity of C. antillanum resins against P. falciparum.

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Diastereomeric Mixture of Calophyllic and Isocalophyllic Acid Ameliorates Scopolamine-Induced Memory Impairment in Mice: Involvement of Antioxidant Defense and Cholinergic Systems

et al. 2019

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A fast and efficient HPLC-PDA–MS method for detection and identification of pyranochromanone acids in Calophyllum species

Cited by 12 publications

References 21 publications

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Antimicrobial Assessment of Resins fromCalophyllum AntillanumandCalophyllum Inophyllum

Diastereomeric Mixture of Calophyllic and Isocalophyllic Acid Ameliorates Scopolamine-Induced Memory Impairment in Mice: Involvement of Antioxidant Defense and Cholinergic Systems

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