A fast and accurate method of detecting Aleutian mink disease virus in blood and tissues of chronically infected mink

Farid, A.; Rupasinghe, Priyanka P.

doi:10.1139/cjm-2016-0567

Cited by 5 publications

(4 citation statements)

References 42 publications

(50 reference statements)

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“…Bacterial, viral and other diseases Among the main epizootic factors that cause deaths of mink kits (from birth to four months of age) on American farms, Wilson et al [2015] mention, inter alia, Aleutian disease which caused 13.5% of falls, viral enteritis causing 10.6% of falls, fatty liver causing 9.6% of falls, epizootic gastroenteritis in mink causing 8.7% of falls and pneumonia caused by the bacterium Pseudomonas aeruginosa -7.7% of the falls, and bacterial enteritis, which is also the cause of approximately 7.7% of the deaths of young mink. Already in 1983, Bøtner and Jørgensen [1983] found that the Aleutian disease virus was responsible for a very high mortality among young mink, amounting to over 70% of individuals in a litter, and Farid and Rupasinghe [2017] report that Aleutian disease is now present in all countries where there is a mink farm.…”

Section: Nutritionmentioning

confidence: 99%

Environmental and Genetic Factors Influencing the Rearing Performance in Mink (Neovison Vison)

Felska-Blaszczyk¹

2022

Acta Sci. Pol. Zootech.

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The aim of this study was to indicate the relationship between various genetic and environmental factors and the results of kits rearing. Among the factors determining the degree of rearing of young kits, genetic factors should be mentioned, especially the color of the mink, and the environmental factors include: duration of labor, age of mothers, mating and laying time, length of pregnancy, litter size, nutrition, body condition the female and her milk yield, indoor microclimate, bacterial and viral diseases, or the care provided by the farm staff. As a result of the analysis, it can be concluded that the number of reared mink babies is influenced by both the color variety and a number of environmental factors, and the mink farmer's task is to get to know all these aspects and treat them comprehensively. The mink breeding should be carried out in such a way that the animal welfare is as good as possible, so that the females give birth to the healthiest and strongest litters.

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Section: Nutritionmentioning

confidence: 99%

Environmental and Genetic Factors Influencing the Rearing Performance in Mink (Neovison Vison)

Felska-Blaszczyk¹

2022

Acta Sci. Pol. Zootech.

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“…The doubtful results were likely caused by low antibody titers, and were considered as positive. Direct AMDV DNA amplification was performed by PCR on plasma and cell-free tissue homogenates in 25 μL total volumes containing (final concentration) 1X PCR buffer, 0.2 mM each dNTP, 400 nM each primer, 1X (12.5 μL) PEC-2 Enhancer and 0.25 μL of Omni Klentaq-LA (DNA Polymerase Technology, http://www.klentaq.com) as previously described [64] with the primer pair 60F/ 60R [65]. Three PCR tests were performed on each sample using 0.5, 1.0 and 2.0 μL of plasma or tissue homogenate.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Dietary supplementation of Ascophylum nodosum improved kidney function of mink challenged with Aleutian mink disease virus

Farid

Smith²

2020

BMC Vet Res

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Background Feed additives which can ease the negative effects of infection by the Aleutian mink disease virus (AMDV) are of interest to mink farmers. The effects of kelp meal (Ascophylum nodosum) supplementation on immune response, virus replication and blood parameters of mink inoculated with AMDV were assessed. AMDV-free black mink (n = 75) were intranasally inoculated with a local strain of AMDV and fed a commercial pellet supplemented with kelp meal at the rates of 1.5% or 0.75% of the feed or were kept as controls (no kelp) for 451 days. Blood was collected on days 0 (pre-inoculation), 31, 56, 99, 155, 366 and 451 post-inoculation (dpi). Results No significant difference was observed among the treatments for the proportion of animals positive for antibodies against the virus measured by the counter-immunoelectrophoresis (CIEP), viremia measured by PCR, antibody titer measured by quantitative ELISA, total serum protein measured by a refractometer or elevated levels of gamma globulin measured by iodine agglutination test at the sampling occasions. At the termination of the experiment on 451 dpi, there were no differences among treatments for antibody titer measured by CIEP, total serum protein, albumin, globulins, albumin:globulin ratio, alkaline phosphatase, gamma-glutamyl transferase, and proportions of PCR positive spleen, lymph node or bone marrow samples, but blood urea nitrogen and creatine levels were significantly lower in the 1.5% kelp supplemented group than in the controls. Conclusion Kelp supplementation improved kidney function of mink infected with AMDV with no effect on liver function, immune response to infection by AMDV or virus replication.

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“…AMDV DNA was amplified by PCR using three volumes (1.5, 2.5 and 3.5 μL) of plasma or cell-free tissue homogenate in 15 μL PCR reaction and primers 60F/60R as previously described [ 26 ]. In 2013 and 2014, 0.5, 1.0 and 2.0 μL of plasma or cell-free tissue homogenate from each animal were directly used in 25 μL PCR reactions with Omni Klentaq-LA enzyme and PCR Enhancer (PEC-2) from DNA Polymerase Technology ( http://www.klentaq.com/ ) as previously described [ 65 ], using the same primer set as before. In both protocols, these three tests were repeated when there was one faint band or no amplification.…”

Section: Methodsmentioning

confidence: 99%

“…klent aq. com/) as previously described [65], using the same primer set as before. In both protocols, these three tests were repeated when there was one faint band or no amplification.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Long-term antibody production and viremia in American mink (Neovison vison) challenged with Aleutian mink disease virus

et al. 2022

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Background Selecting American mink (Neovison vison) for tolerance to Aleutian mink disease virus (AMDV) has gained popularity in recent years, but data on the outcomes of this activity are scant. The objectives of this study were to determine the long-term changes in viremia, seroconversion and survival in infected mink. Mink were inoculated intranasally with a local isolate of Aleutian mink disease virus (AMDV) over 4 years (n = 1742). The animals had been selected for tolerance to AMDV for more than 20 years (TG100) or were from herds free of AMDV (TG0). The progenies of TG100 and TG0, and their crosses with 25, 50 and 75% tolerance ancestry were also used. Blood samples were collected from each mink up to 14 times until 1211 days post-inoculation (dpi) and were tested for viremia by PCR and for anti-AMDV antibodies by counter-immunoelectrophoresis (CIEP). Viremia and CIEP status were not considered when selecting replacements. Low-performing animals were pelted and the presence of antibodies in their blood and antibody titer were measured by CIEP, and viremia and viral DNA in seven organs (n = 936) were tested by PCR. Results The peak incidences of viremia (66.7%) and seropositivity (93.5%) were at 35 dpi. The incidence of viremia decreased over time while the incidence of seroconversion increased. The least-squares means of the incidence of PCR positive of lymph node (0.743) and spleen (0.656) were significantly greater than those of bone marrow, liver, kidneys, lungs and small intestine (0.194 to 0.342). Differences in tolerant ancestry were significant for every trait measured. Incidences of viremia over time, terminal viremia, seropositivity over time, AMDV DNA in organs and antibody titer were highest in the susceptible groups (TG0 or TG25) and lowest in the tolerant groups (TG100 or TG75). Conclusion Previous history of selection for tolerance resulted in mink with reduced viral replication and antibody titer. Viremia had a negative effect and antibody production had a positive effect on survival and productivity.

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A fast and accurate method of detecting Aleutian mink disease virus in blood and tissues of chronically infected mink

Cited by 5 publications

References 42 publications

Environmental and Genetic Factors Influencing the Rearing Performance in Mink (Neovison Vison)

Environmental and Genetic Factors Influencing the Rearing Performance in Mink (Neovison Vison)

Dietary supplementation of Ascophylum nodosum improved kidney function of mink challenged with Aleutian mink disease virus

Long-term antibody production and viremia in American mink (Neovison vison) challenged with Aleutian mink disease virus

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