A Family of Turandot-Related Genes in the Humoral Stress Response of Drosophila

Ekengren, Sophia; Hultmark, Dan

doi:10.1006/bbrc.2001.5067

Cited by 153 publications

(145 citation statements)

References 16 publications

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“…Many candidate genes map within these QTL ranges (supplementary material TableS3). All of these candidate genes were either heat upregulated (Leemans et al, 2000;Ekengren and Hultmark, 2001;Sørensen et al, 2005) or differed in their respective expression levels between heat-resistant and heat-sensitive lines in D. melanogaster (McKechnie et al, 1998;Nielsen et al, 2006;Sørensen et al, 2007;Norry et al, 2009). Some of these candidate loci are involved in metabolic (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, Rako et al (Rako et al, 2007) observed that increased heat resistance was associated with one allele of hsr-omega. Other candidate genes are also included on the same QTL region where hsr-omega maps (supplementary material TableS3), such as Turandot-related genes, which are known to be involved in the cellular response to heat (Ekengren and Hultmark, 2001). This result supports previous findings showing that the region where hsr-omega and other candidate genes map on the right arm of chromosome 3 represents a consistent QTL for thermotolerance not only in the adult fly, but also in the pre-adult stage(s) in D. melanogaster (Table 2).…”

Section: Discussionmentioning

confidence: 99%

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Heat stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines ofDrosophila melanogaster

Sambucetti

Loeschcke

Norry

2013

Journal of Experimental Biology

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SUMMARYIn insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitative trait loci (QTL) for heat-stress survival in larvae of Drosophila melanogaster, with and without a mild-heat-stress pre-treatment. Two sets of recombinant inbred lines derived from lines artificially selected for high and low levels of knockdown resistance to high temperature in young flies were used as the mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival (with and without a heat pre-treatment) except for males from one set of lines. Several QTL were identified involving all three major chromosomes. Most QTL for larval thermotolerance overlapped with thermotolerance QTL identified in previous studies for adults, indicating that heat-stress resistance is not genetically independent between life cycle stages because of either linkage or pleiotropy. The sign of the effects of some QTL alleles differed both between the sexes and between life stages. Supplementary material available online at

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heat stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines ofDrosophila melanogaster

Sambucetti

Loeschcke

Norry

2013

Journal of Experimental Biology

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“…Three genes (TotA, TotC and TotM) from the Turandot family of stress proteins were also upregulated after cold exposures in our study. Previous work has found that the expression of genes in this family can be induced in D. melanogaster by varied stressors, including heat shock, cold shock, septic injury or bacterial infection (Ekengren and Hultmark, 2001). In particular, TotA was upregulated in response to all three cold stresses in our study, with a 15-fold up-regulation after multiple cold exposures, whereas TotM was only up-regulated in response to multiple cold exposures, and TotC was up-regulated after both multiple and prolonged cold exposures.…”

Section: Stress-related Genes Up-regulated In Response To Cold Exposurementioning

confidence: 99%

Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster

Zhang

Marshall

Westwood

et al. 2011

Journal of Experimental Biology

105

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SUMMARYInsects in the field are exposed to multiple bouts of cold, and there is increasing evidence that the fitness consequences of repeated cold exposure differ from the impacts of a single cold exposure. We tested the hypothesis that different kinds of cold exposure (in this case, single short, prolonged and repeated cold exposure) would result in differential gene expression. We exposed 3day old adult female wild-type Drosophila melanogaster (Diptera: Drosophilidae) to -0.5°C for a single 2h exposure, a single 10h exposure, or five 2h exposures on consecutive days, and extracted RNA after 6h of recovery. Global gene expression was quantified using an oligonucleotide microarray and validated with real-time PCR using different biological replicates. We identified 76 genes upregulated in response to multiple cold exposure, 69 in response to prolonged cold exposure and 20 genes upregulated in response to a single short cold exposure, with a small amount of overlap between treatments. Three genesTurandot A, Hephaestus and CG11374 -were upregulated in response to all three cold exposure treatments. Key functional groups upregulated include genes associated with muscle structure and function, the immune response, stress response, carbohydrate metabolism and egg production. We conclude that cold exposure has wide-ranging effects on gene expression in D. melanogaster and that increased duration or frequency of cold exposure has impacts different to those of a single short cold exposure. This has important implications for extrapolating laboratory studies of insect overwintering that are based on only a single cold exposure. Supplementary material available online at

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“…These peptides are induced by the MEKK pathway (Brun et al 2006) and are also induced in hop Tum-l and Nurf301 mutants (Kwon et al 2008). Turandot peptides are secreted into the hemolymph after stress induction such as heat shock or oxidative stress and may act in a similar way to heat-shock proteins (Ekengren and Hultmark 2001). Similarly, many glutathione transferase genes are highly induced, further implying an activation of oxidative stress pathways.…”

Section: Ex1mentioning

confidence: 99%

Role of Elongator Subunit Elp3 in Drosophila melanogaster Larval Development and Immunity

et al. 2011

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The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for $24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino.

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A Family of Turandot-Related Genes in the Humoral Stress Response of Drosophila

Cited by 153 publications

References 16 publications

Heat stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines ofDrosophila melanogaster

Heat stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines ofDrosophila melanogaster

Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster

Role of Elongator Subunit Elp3 in Drosophila melanogaster Larval Development and Immunity

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