A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process

Abstract

The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer...

Ultrasensitive miRNA Detection Based on Magnetic Upconversion Nanoparticle Enhancement and CRISPR/Cas13a-Driven Signal Amplification

“…Different from traditional organic fluorescent dyes and quantum dots, upconversion nanoparticle (UCNP) is a new type of nano luminescent material, which follows the anti-Stokes effect and can convert low-energy light (e.g., 808/980 near-infrared light) into high-energy light (e.g., blue and green light) with advantages of stable optical properties, low background fluorescence, no photobleaching, low toxicity, etc. , Therefore, based on the principle of luminescence resonance energy transfer (LRET), some researchers have replaced traditional organic dyes with UCNPs as luminescence donors and applied BHQ1, graphene, − gold nanoparticles (AuNPs), − and manganese dioxide (MnO 2 ) as luminescence receptors for miRNA detection and biological imaging.…”

On-Site Remote Monitoring System with NIR Signal-Based Detection of Infectious Disease Virus in Opaque Salivary Samples

“…The nanoprobe consists of an upconversion nanoparticle (UCNP) of NaYF 4 :Yb, Tm, which emits 800 nm light when excited at 980 nm. The NIR signal nanoprobe effectively minimizes the interference signal of clinical saliva samples, which contain biological interfering agents with low autofluorescence, scattering, and absorption within the NIR window. − The NIR signal nanoprobe-assembled LFA exhibited a 32-fold lower limit of detection (LOD) in detecting FMDVs, including all isolates that occurred in the Republic of Korea from 2010 to 2017 compared to a commercial LFA. We achieved the lowest-ever LOD in detecting FMDVs in clinical saliva samples among LFAs reported to date. , The NIR signal LFA successfully detected FMDVs even in dark brown saliva samples with a high sensitivity of 96.9%, specificity of 100.0%, and accuracy of 97.9%.…”