A facile route to prepare colorless Ag-Cu nanoparticle dispersions with elevated antibacterial effects

Zhu, Yan; Zhou, Fang; Hu, Junqiang; Yang, Longlai; Yang, De‐Quan; Sacher, E.

doi:10.1016/j.colsurfa.2021.127116

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…The antibacterial activity of Ag-Cu films deposited on polymeric or metallic materials has been previously demonstrated [63][64][65][66][67][68][69], where the advantage of using bimetallic particles instead of single materials is related to the enhanced charge transfer mechanism when alloys are used [23,[70][71][72]. Moreover, Ag-Cu nanoalloys have demonstrated enhanced resistance to electromigration and oxidation [73], high stability, and improved bactericidal effects compared to pure Ag [74]. A theoretical study also concluded that using bimetallic NPs using immiscible metals, such as Ag and Cu, is an appropriate means to customize the interaction between the NPs and the cellular and bacterial membranes [75] that might promote the adhesion and internalization of the NPs and therefore their effect.…”

Section: Introductionmentioning

confidence: 99%

Biocide effect against SARS-CoV-2 and ESKAPE pathogens of a noncytotoxic silver–copper nanofilm

et al. 2021

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Nanometric materials with biocidal properties effective against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) and pathogenic bacteria could be used to modify surfaces, reducing the risk of touching transmission. In this work, we showed that a nanometric layer of bimetallic AgCu can be effectively deposited on polypropylene (PP) fibers. The virucidal properties of the AgCu nanofilm were evaluated by comparing the viral loads remaining on uncoated and coated PP after contact times between 2 and 24 h. Quantification of virion numbers for different initial concentrations indicated a reduction of more than 95% after 2 h of contact. The bactericidal action of the AgCu nanofilm was also confirmed by inoculating uncoated and coated PP with a pool of pathogenic bacteria associated with pneumonia (ESKAPE). Meanwhile, no cytotoxicity was observed for human fibroblasts and keratinocyte cells, indicating that the nanofilm could be in contact with human skin without threat. The deposition of the AgCu nanofilm on the nonwoven component of reusable cloth masks might help to prevent virus and bacterial infection while reducing the pollution burden related to the disposable masks. The possible mechanism of biocide contact action was studied by quantum chemistry calculations that show that the addition of Ag and/or Cu makes the polymeric fiber a better electron acceptor. This can promote the oxidation of the phospholipids present at both the virus and bacterial membranes. The rupture at the membrane exposes and damages the genetic material of the virus. More studies are needed to determine the mechanism of action, but the results reported here indicate that Cu and Ag ions are good allies, which can help protect us from the virus that has caused this disturbing pandemic.

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Section: Introductionmentioning

confidence: 99%

Biocide effect against SARS-CoV-2 and ESKAPE pathogens of a noncytotoxic silver–copper nanofilm

et al. 2021

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“…The color change of the Ag NPs upon adding Cu 2+ , varying with time from yellow to almost colorless after 8 days ( Figure S1 ), is attributed to the reaction of Cu 2+ with Ag NPs. As in our previous work [ 5 , 30 ], the color of the AgNPs’ dispersion is not only related to the size and density of the NPs, but also to the agglomeration and dispersion of the NPs, so it is difficult to directly judge or determine the cause of color changes over time. However, for a given Ag concentration, the color change can be attributed to the AgCu NAs formation and the associated size change [ 5 , 30 ].…”

Section: Discussionmentioning

confidence: 98%

“…Recent studies [ 5 , 30 ] found that AgCu NAs can be synthesized by the Ag NP catalysis of Cu 2+ at 85 °C, which showed a greatly enhanced antibacterial efficacy. It is believed that the enhanced antibacterial mechanism partly involves Ag + released during AgCu NA formation.…”

Section: Introductionmentioning

confidence: 99%

AgCu NP Formation by the Ag NP Catalysis of Cu Ions at Room Temperature and Their Antibacterial Efficacy: A Kinetic Study

Tao

Zhou²,

Wang³

et al. 2022

Molecules

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Although a facile route to prepare AgCu nanoalloys (NAs) with enhanced antibacterial efficacy using Ag NP catalysis of Cu ions at elevated temperatures was previously developed, its detailed reaction process is still unclear due to the fast reaction process at higher temperatures. This work found that AgCu NAs can also be synthesized by the same process but at room temperature. AgCu NAs formation kinetics have been studied using UV–Visible spectra and Transmission Electron Microscopy (TEM), where formation includes Cu2+ deposition onto the Ag NP surface and Ag+ release, reduction, and agglomeration to form new Ag NPs; this is followed by a redistribution of the NA components and coalescence to form larger AgCu NPs. It is found that SPR absorption is linear with time early in the reaction, as expected for both pseudo-first-order (PFO) and pseudo-second-order (PSO) kinetics; neither model is followed subsequently due to contributions from newly formed Ag NPs and AgCu NAs. The antibacterial efficacy of the AgCu NAs thus formed was estimated, with a continuous increase over the whole alloying process, demonstrating the correlation of antibacterial efficacy with the extent of AgCu NA formation and Ag+ release.

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“…In the present work, we compare the antibacterial efficacy and cytotoxicity of AgCu and AgFe NAs, based on our previous papers [ 33 , 34 , 35 ], prepared in the same manner and using the same stabilizers, so as to eliminate the effect of the changing physicochemical properties on the cytotoxicity and antibacterial efficacy [ 25 , 26 ]. Therefore, their antibacterial efficacy and cytotoxicity are comparable, showing similar trends, whereby both MIC and MDC (minimum death concentration) follow the order of Ag NPs > AgCu NAs > AgFe NAs .…”

Section: Introductionmentioning

confidence: 99%

“…We offer explanations as to why this is so. Our intention in this work is to focus on the correlation between the antibacterial efficacy and cytotoxicity, rather than on their preparation and physicochemistry, which were addressed in our previous publications [ 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Antibacterial Efficacy of AgCu and AgFe NanoAlloys: A Comparative Study

Zhou¹,

Kostantin

Yang

et al. 2022

Antibiotics

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Although Ag nanoparticles (NPs) have been widely applied in daily life and in biomedical and industrial fields, there is a demand for Ag-based bimetallic nanoalloys (NAs), such as AgCu and AgFe, due to their enhanced antibacterial efficacy and reduced Ag consumption. In this work, we present a comparison study on the antibacterial efficacy and cytotoxicity rates of Ag NPs and AgCu and AgFe NAs to L929 mouse fibroblast cells using the CCK-8 technique based on the relative cell viability. The concept of the minimum death concentration (MDC) is introduced to estimate the cytotoxicity to the cells. It is found that the minimum inhibitory concentrations (MICs) of the NPs against E. coli and S. aureus decrease with the addition of both Cu and Fe. There is a strong correlation between the MDC and MIC, implying that the mechanisms of both antibacterial efficacy and cytotoxicity are similar. The enhanced antibacterial efficacy to bacteria and cytotoxicity toward the cell are attributed to Ag+ release. The following order is found for both the MIC and MDC: AgFe < AgCu < Ag NPs. However, there is no cytotoxicity to the L929 cells for AgFe and AgCu NAs at their MIC Ag concentrations against S. aureus.

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A facile route to prepare colorless Ag-Cu nanoparticle dispersions with elevated antibacterial effects

Cited by 13 publications

References 40 publications

Biocide effect against SARS-CoV-2 and ESKAPE pathogens of a noncytotoxic silver–copper nanofilm

Biocide effect against SARS-CoV-2 and ESKAPE pathogens of a noncytotoxic silver–copper nanofilm

AgCu NP Formation by the Ag NP Catalysis of Cu Ions at Room Temperature and Their Antibacterial Efficacy: A Kinetic Study

Cytotoxicity and Antibacterial Efficacy of AgCu and AgFe NanoAlloys: A Comparative Study

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