A facile phototheranostic nanoplatform integrating NIR-II fluorescence/PA bimodal imaging and image-guided surgery/PTT combinational therapy for improved antitumor efficacy

Han, Xiuping; Fang, Wenyou; Zhang, Tianqi; Zhong, Xuan; Qian, Kun; Jiang, Zhitao; Hu, Rongfeng; Shao, Guoqiang; Zhang, Lei; Zhang, Qing

doi:10.1016/j.jmst.2022.05.027

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…The ICG loading capacity (LC) was estimated by evaluating the ICG to ICG: HFn mass ratio (%) in the final preparation, as described in the literature. 39 , 40 Full HFn-ICG characterization including encapsulation efficiency, drug release and NP stability are reported in the cited manuscript. 11 …”

Section: Methodsmentioning

confidence: 99%

Combined Ferritin Nanocarriers with ICG for Effective Phototherapy Against Breast Cancer

Sitia,

Saccomandi,

Bianchi

et al. 2024

IJN

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Introduction Photodynamic Therapy (PDT) is a promising, minimally invasive treatment for cancer with high immunostimulatory potential, no reported drug resistance, and reduced side effects. Indocyanine Green (ICG) has been used as a photosensitizer (PS) for PDT, although its poor stability and low tumor-target specificity strongly limit its efficacy. To overcome these limitations, ICG can be formulated as a tumor-targeting nanoparticle (NP). Methods We nanoformulated ICG into recombinant heavy-ferritin nanocages (HFn-ICG). HFn has a specific interaction with transferrin receptor 1 (TfR1), which is overexpressed in most tumors, thus increasing HFn tumor tropism. First, we tested the properties of HFn-ICG as a PS upon irradiation with a continuous-wave diode laser. Then, we evaluated PDT efficacy in two breast cancer (BC) cell lines with different TfR1 expression levels. Finally, we measured the levels of intracellular endogenous heavy ferritin (H-Fn) after PDT treatment. In fact, it is known that cells undergoing ROS-induced autophagy, as in PDT, tend to increase their ferritin levels as a defence mechanism. By measuring intracellular H-Fn, we verified whether this interplay between internalized HFn and endogenous H-Fn could be used to maximize HFn uptake and PDT efficacy. Results We previously demonstrated that HFn-ICG stabilized ICG molecules and increased their delivery to the target site in vitro and in vivo for fluorescence guided surgery. Here, with the aim of using HFn-ICG for PDT, we showed that HFn-ICG improved treatment efficacy in BC cells, depending on their TfR1 expression. Our data revealed that endogenous H-Fn levels were increased after PDT treatment, suggesting that this defence reaction against oxidative stress could be used to enhance HFn-ICG uptake in cells, increasing treatment efficacy. Conclusion The strong PDT efficacy and peculiar Trojan horse-like mechanism, that we revealed for the first time in literature, confirmed the promising application of HFn-ICG in PDT.

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Section: Methodsmentioning

confidence: 99%

Combined Ferritin Nanocarriers with ICG for Effective Phototherapy Against Breast Cancer

Sitia,

Saccomandi,

Bianchi

et al. 2024

IJN

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“…For example, molecular fluorescent dyes operating in the visible range play a crucial role in fluorescence analysis, flow cytometry, cell imaging, polymerase chain reaction, gene sequencing, , etc. Moreover, fluorescence imaging technology has emerged as a powerful tool for disease diagnosis and image-guided surgeries. − Surgical procedures are pivotal for tumor treatment, however, data regarding different cancer types show that 70–80% of tumors still exhibit positive margins after resection, indicating the presence of cancer cells at the surgical margins. To decrease the recurrence rate and improve postoperative quality of life, precise tumor resection has become a prevailing trend, highlighting the importance of intraoperative guidance for surgeons.…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Nanocrystals for Deep-Tissue In Vivo Luminescence Imaging in the Near-Infrared Region

Yang,

Jiang,

Zhang

2023

Chem. Rev.

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In vivo imaging technologies have emerged as a powerful tool for both fundamental research and clinical practice. In particular, luminescence imaging in the tissuetransparent near-infrared (NIR, 700−1700 nm) region offers tremendous potential for visualizing biological architectures and pathophysiological events in living subjects with deep tissue penetration and high imaging contrast owing to the reduced light−tissue interactions of absorption, scattering, and autofluorescence. The distinctive quantum effects of nanocrystals have been harnessed to achieve exceptional photophysical properties, establishing them as a promising category of luminescent probes. In this comprehensive review, the interactions between light and biological tissues, as well as the advantages of NIR light for in vivo luminescence imaging, are initially elaborated. Subsequently, we focus on achieving deep tissue penetration and improved imaging contrast by optimizing the performance of nanocrystal fluorophores. The ingenious design strategies of NIR nanocrystal probes are discussed, along with their respective biomedical applications in versatile in vivo luminescence imaging modalities. Finally, thought-provoking reflections on the challenges and prospects for future clinical translation of nanocrystal-based in vivo luminescence imaging in the NIR region are wisely provided.

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“…However, a single imaging modality cannot afford comprehensive tumor information. 56 Photoacoustic imaging (PAI) induced by NIR-II utilizes acoustic waves converted by photothermal transformation to detect ultrasound (US) signals. 57 Benefit from ultra-high penetrating advantage of US, PAI can recognize deep tumor contour information with high resolution.…”

Section: Polymers With Pa/ptt Dual Functionsmentioning

confidence: 99%

“…NIR‐II fluorescence has diminished tissue absorption and deeper penetration depth. However, a single imaging modality cannot afford comprehensive tumor information 56 . Photoacoustic imaging (PAI) induced by NIR‐II utilizes acoustic waves converted by photothermal transformation to detect ultrasound (US) signals 57 .…”

Section: Polymers Applied As Ptasmentioning

confidence: 99%

Polymer materials for constructing therapeutical nanoparticles in photothermal therapy

Li,

Liu,

Zhang

2023

Journal of Polymer Science

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Photothermal therapy (PTT) ablates tumors by thermal effects of photothermal agents (PTAs), and attracts wide attention due to the non‐invasive characteristic. The ideal PTAs are expected to have high photothermal conversion effect under NIR irradiation, as well as targeting abilities and good biocompatibility satisfying the need of application in vivo. Nanoparticles (NPs) are commonly used as anti‐tumor materials, and plenty of researches on therapeutical NPs for PTT treatment have been developed. Among various building blocks for photothermal NPs, polymer materials for biomedical applications have great advantages due to their negligible toxicity, flexibility for functional modification, and ability to integrate multiple therapeutic strategies. This review focuses on the polymer materials utilized in photothermal NP designing, including their application as excellent carriers and powerful PTAs with great PTT effects. Furthermore, the synergy therapy based on polymeric nanoplatform for enhancing PTT therapeutic efficiency will be introduced.

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A facile phototheranostic nanoplatform integrating NIR-II fluorescence/PA bimodal imaging and image-guided surgery/PTT combinational therapy for improved antitumor efficacy

Cited by 10 publications

References 30 publications

Combined Ferritin Nanocarriers with ICG for Effective Phototherapy Against Breast Cancer

Combined Ferritin Nanocarriers with ICG for Effective Phototherapy Against Breast Cancer

Nanocrystals for Deep-Tissue In Vivo Luminescence Imaging in the Near-Infrared Region

Polymer materials for constructing therapeutical nanoparticles in photothermal therapy

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