A facile one-stage treatment of critical bone defects using a calcium sulfate/hydroxyapatite biomaterial providing spatiotemporal delivery of bone morphogenic protein–2 and zoledronic acid

Raina, Deepak; Matuszewski, Lucas Maximilian; Vater, Corina; Bolte, Julia; Isaksson, Hanna; Lidgren, Lars; Tägil, Magnus; Zwingenberger, Stefan

doi:10.1126/sciadv.abc1779

Cited by 49 publications

(43 citation statements)

References 43 publications

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“…Surgeries were performed in prone position as described previously [ 22 ]. Briefly, the right hind limb of the animal was shaved and disinfected, and an approximately 3 cm long skin and fascia incision was made along the length of the right femur.…”

Section: Methodsmentioning

confidence: 99%

Dose-Dependent Effects of a Novel Selective EP4 Prostaglandin Receptor Agonist on Treatment of Critical Size Femoral Bone Defects in a Rat Model

et al. 2021

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Difficulties in treating pseudarthrosis and critical bone defects are still evident in physicians’ clinical routines. Bone morphogenetic protein 2 (BMP-2) has shown promising osteoinductive results but also considerable side effects, not unexpected given that it is a morphogen. Thus, the bone regenerative potential of the novel selective, non-morphogenic EP4 prostaglandin receptor agonist KMN-159 was investigated in this study. Therefore, mineralized collagen type-1 matrices were loaded with different amounts of BMP-2 or KMN-159 and implanted into a 5 mm critical-sized femoral defect in rats. After 12 weeks of observation, micro-computed tomography scans were performed to analyze the newly formed bone volume (BV) and bone mineral density (BMD). Histological analysis was performed to evaluate the degree of defect healing and the number of vessels, osteoclasts, and osteoblasts. Data were evaluated using Kruskal-Wallis followed by Dunn’s post hoc test. As expected, animals treated with BMP-2, the positive control for this model, showed a high amount of newly formed BV as well as bone healing. For KMN-159, a dose-dependent effect on bone regeneration could be observed up to a dose optimum, demonstrating that this non-morphogenic mechanism of action can stimulate bone formation in this model system.

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Section: Methodsmentioning

confidence: 99%

Dose-Dependent Effects of a Novel Selective EP4 Prostaglandin Receptor Agonist on Treatment of Critical Size Femoral Bone Defects in a Rat Model

et al. 2021

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“…Bone tissue engineering is considered a promising approach for bone tissue repair and regeneration using a variety of bioactive materials. [65] Nanotechnologies appear to have an important and promising role in bone tissue engineering. The use of nanostructured scaffolds provides an appropriate environment for cell proliferation, differentiation, adhesion, and bone formation by mimicking the biological micro-and nano-environments, as well as controlled delivery of drugs and growth factors in the lesion site.…”

Section: Bone Regenerationmentioning

confidence: 99%

Nanotechnology-based drug delivery systems in orthopedics

Güven

2021

Jt Dis Relat Surg

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Nanotechnology is a multidisciplinary branch of science which involves the manipulation and control of matter at the nanometer scale to produce new structures, materials, and devices. The concept of nanotechnology was first introduced by Richard P. Feynman [1] in 1959. Since then, nanotechnology has become a research area which promises advancements in many aspects of human life, including electronics, agriculture, transportation, food industry, communication, energy, biological sciences, and medicine. Nanomedicine, the application of nanotechnology in the field of medicine, has the potential to make a great impact on human health and has already impacted and reshaped many aspects of clinical practice and research. Nanotechnology-based materials with unique physical, chemical, and biological characteristics offer a variety of new approaches for clinical practices ranging from prevention to treatment and diagnosis of many diseases and conditions. [2][3][4][5] Among all the applications of nanotechnology in medicine, nano-based drugIn recent years, nanotechnology has led to significant scientific and technological advances in diverse fields, specifically within the field of medicine. Owing to the revolutionary implications in drug delivery, nanotechnology-based drug delivery systems have gained an increasing research interest in the current medical field. A variety of nanomaterials with unique physical, chemical and biological properties have been engineered to develop new drug delivery systems for the local, sustained and targeted delivery of drugs with improved therapeutic efficiency and less or no toxicity, representing a very promising approach for the effective management of diseases. The utility of nanotechnology, particularly in the field of orthopedics, is a topic of extensive research. Nanotechnology has a great potential to revolutionize treatment, diagnostics, and research in the field of orthopedics. Nanophase drug delivery has shown great promise in their ability to deliver drugs at nanoscale for a variety of orthopedic applications. In this review, we discuss recent advances in the field of nanostructured drug delivery systems for orthopedic applications.

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“…Surgeries were performed in prone position as described previously [11,12]. The right hind limb of the animal was shaved and disinfected, and an approximately 3 cm skin and fascia incision was made along the length of the right femur.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering

et al. 2021

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The purpose of this study was to investigate, in vitro and in vivo, the suitability of chitosan (CHS) scaffolds produced by the net-shape-nonwoven (NSN) technology, for use as bone graft substitutes in a critical-size femoral bone defect in rats. For in vitro investigations, scaffolds made of CHS, mineralized collagen (MCM), or human cancellous bone allograft (CBA) were seeded with human telomerase-immortalized mesenchymal stromal cells (hTERT-MSC), incubated for 14 days, and thereafter evaluated for proliferation and osteogenic differentiation. In vivo, CHS, MCM and CBA scaffolds were implanted into 5 mm critical-size femoral bone defects in rats. After 12 weeks, the volume of newly formed bone was determined by microcomputed tomography (µCT), while the degree of defect healing, as well as vascularization and the number of osteoblasts and osteoclasts, was evaluated histologically. In vitro, CHS scaffolds showed significantly higher osteogenic properties, whereas treatment with CHS, in vivo, led to a lower grade of bone-healing compared to CBA and MCM. While chitosan offers a completely new field of scaffold production by fibers, these scaffolds will have to be improved in the future, regarding mechanical stability and osteoconductivity.

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A facile one-stage treatment of critical bone defects using a calcium sulfate/hydroxyapatite biomaterial providing spatiotemporal delivery of bone morphogenic protein–2 and zoledronic acid

Cited by 49 publications

References 43 publications

Dose-Dependent Effects of a Novel Selective EP4 Prostaglandin Receptor Agonist on Treatment of Critical Size Femoral Bone Defects in a Rat Model

Dose-Dependent Effects of a Novel Selective EP4 Prostaglandin Receptor Agonist on Treatment of Critical Size Femoral Bone Defects in a Rat Model

Nanotechnology-based drug delivery systems in orthopedics

Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering

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