A facile method of achieving low surface coverage of Au nanoparticles on an indium tin oxide electrode and its application to protein detection

Aziz, Md. Abdul; Patra, Srikanta; Yang, Haesik

doi:10.1039/b808026g

Cited by 73 publications

(61 citation statements)

References 33 publications

(7 reference statements)

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“…To test the feasibility of the sensor, a sandwich-type immunosensor for detecting mouse IgG was designed. Biotinylated antimouse IgG was immobilized on avidin-, biotin-, and APPA-modified ITO electrodes to capture target mouse IgG [5,6,19]. ALP-conjugated antimouse IgG was attached after the mouse IgG was captured [5,6,19].…”

Section: Resultsmentioning

confidence: 99%

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Platform for Highly Sensitive Alkaline Phosphatase‐Based Immunosensors Using 1‐Naphthyl Phosphate and an Avidin‐Modified Indium Tin Oxide Electrode

Aziz

Qaium

et al. 2009

Electroanalysis

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We report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.

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Section: Resultsmentioning

confidence: 99%

“…However, in many cases, low electrocatalytic activities of ITO electrodes require modification of the ITO electrodes with electrocatalytic materials such as ferrocene [5,12], carbon nanotubes [19], and gold nanoparticles [6,20,21]. The modification makes the preparation of sensing surfaces more complex and the sensors impractical.…”

Section: Introductionmentioning

confidence: 99%

Platform for Highly Sensitive Alkaline Phosphatase‐Based Immunosensors Using 1‐Naphthyl Phosphate and an Avidin‐Modified Indium Tin Oxide Electrode

Aziz

Qaium

et al. 2009

Electroanalysis

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“…2a). In our previous studies, the electrocatalytic properties of ITO electrodes were improved by partial modification of ITO electrodes with ferrocene [11], carbon nanotubes [12], or gold nanoparticles [13]. In this study, the intrinsic low electrocatalytic activity of ITO electrodes was utilized by not modifying ITO electrodes with electrocatalytic materials.…”

Section: Electrocatalytic Properties Of Dendrimer-modified Ito Electrmentioning

confidence: 97%

Strategy for Low Background‐Current Levels in the Electrochemical Biosensors Using Horse‐Radish Peroxidase Labels

et al. 2009

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This article describes an electrochemical strategy to achieve low background-current levels in horse-radish peroxidase (HRP)-based electrochemical immunosensors. The strategy consists of (i) the use of an HRP substrate/product redox couple whose formal potential is high and (ii) the use of an electrode that shows moderate electrocatalytic activity for the redox couple. The strategy is proved by a model biosensor using a catechol/o-benzoquinone redox couple and an indium tin oxide (ITO) electrode. The combined effect of high formal potential and moderate electrocatalytic activity allows o-benzoquinone electroreduction with minimal catechol electrooxidation and H 2 O 2 electroreduction. The detection limit for mouse-IgG is 100 pg/mL.

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“…21 In some cases, it is required to obtain higher or lower electrocatalytic activities of ITO electrodes, depending on their application. Higher electrocatalytic activities can be obtained by modifying ITO electrodes with more electrocatalytic materials such as gold nanoparticles, 22 carbon nanotubes, 23 and graphenes. 24 However, it would be better to obtain higher electrocatalytic activities via simple pretreatment of ITO electrodes.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Pretreatments on Indium-Tin Oxide Electrodes

Choi¹,

Jo²,

Yang

2013

Bulletin of the Korean Chemical Society

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The effect of pretreatment on indium-tin oxide (ITO) electrodes has been rarely studied, although that on metal and carbon electrodes has been enormously done. The electrochemical and surface properties of ITO electrodes are investigated after 6 different pretreatments. The electrochemical behaviors for oxygen reduction, Ru(NH3)6 3+ reduction, Fe(CN)6 3− reduction, and p-hydroquinone oxidation are compared, and the surface roughness, hydrophilicity, and surface chemical composition are also compared. Oxygen reduction, Fe(CN)6 3− reduction, and p-hydroquinone oxidation are highly affected by the type of the pretreatment, whereas Ru(NH3)6 3+ reduction is almost independent of it. Interestingly, oxygen reduction is significantly suppressed by the treatment in an HCl solution. The changes in surface roughness and composition are not high after each pretreatment, but the change in contact angle is substantial in some pretreatments.

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A facile method of achieving low surface coverage of Au nanoparticles on an indium tin oxide electrode and its application to protein detection

Abstract: Low surface coverage of Au nanoparticles on an indium tin oxide electrode for sensitive electrochemical detection was achieved using electrostatic adsorption of AuCl(4)(-) followed by reduction.

Cited by 73 publications

References 33 publications

Platform for Highly Sensitive Alkaline Phosphatase‐Based Immunosensors Using 1‐Naphthyl Phosphate and an Avidin‐Modified Indium Tin Oxide Electrode

Platform for Highly Sensitive Alkaline Phosphatase‐Based Immunosensors Using 1‐Naphthyl Phosphate and an Avidin‐Modified Indium Tin Oxide Electrode

Strategy for Low Background‐Current Levels in the Electrochemical Biosensors Using Horse‐Radish Peroxidase Labels

Effect of Different Pretreatments on Indium-Tin Oxide Electrodes

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