A facile fluorescent sensor based on carbon dots and Fe3O4 nanoplates for the detection of hyaluronidase activity

Chen, Qiutong; Rong, Sihan; Yao, Chuang; Xu, Guanhong; Xie, Zhen; Yang, Jing; Sun, Yong; Hu, Qin; Wei, Fangdi

doi:10.1016/j.snb.2021.130434

Cited by 5 publications

(1 citation statement)

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“…Through the degradation of HA, HAase can increase the permeability of the cell membrane, reduce the viscosity of the extracellular environment, and enhance the diffusion of drugs in cells . HAase is closely related to the physiological and pathological processes such as maintenance of tissue elastic viscosity, embryogenesis, inflammation, and wound healing. , Overexpression of HAase is associated with bladder, rectal, and prostate cancers, so HAase is considered as an important tumor marker. − Methods for HAase activity detection include colorimetry, viscosity, fluorescence, surface-enhanced Raman scattering, and electrochemiluminescence. − However, these methods often have the problems of expensive instruments, long analysis time, and complicated operation. Therefore, it is highly desirable to develop a simple and efficient method for detecting the HAase activity.…”

Section: Introductionmentioning

confidence: 99%

Photocurrent Polarity Switchable Sensing of Hyaluronidase Activity by Regulating Electrostatic Interactions between Two Semiconductors

Liao,

Dai,

Liu

et al. 2023

Anal. Chem.

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Photocurrent polarity switchable photoelectrochemical (PEC) sensing has superior accuracy and anti-interference ability to conventional PEC sensing. The development of a novel strategy for photocurrent polarity switchable sensing is of great interest. Herein, a novel strategy for photocurrent polarity switchable sensing is reported by regulating electrostatic interactions between two semiconductor photoactive materials. Hyaluronic acid (HA)-modified CuO nanosheets show a negatively charged surface, which prevents the attachment of CuO nanosheets to negatively charged CdS nanodendrite-modified photoelectrodes because of the strong electrostatic repulsion. In the presence of hyaluronidase (HAase), the specific hydrolysis of HA on the surface of CuO by HAase can yield a positively charged surface, so CuO can be attached to a CdS-modified photoelectrode via electrostatic attraction, leading to photocurrent polarity switching. The photocurrent polarity switchable detection of HAase activity is achieved with an ultralow detection limit of 2 × 10 −3 U mL −1 and a wide linear detection range between 0.01 and 100 U mL −1 . This work provides a new and effective photocurrent polarity switching strategy for PEC sensing and a simple and efficient method for detecting HAase activity.

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