A facile Agrobacterium-mediated transformation method for the model unicellular green algae Chlamydomonas reinhardtii
Truyen N. Quach,
Shirley J. Sato,
Mark R. Behrens
et al.
Abstract:A reliable and simple Agrobacterium-mediated transformation system for the unicellular green algae model organism Chlamydomonas reinhardtii has been developed. The protocol has been successfully employed with both neomycin phosphotransferase II (nptII) and the phleomycin resistance (bleI) genes coupled with the selective agents paromomycin and zeocin, respectively. A set of binary vectors were assembled that carry the selectable marker cassettes under control either of the Rbcs2 alone or fused to the HSP270A l… Show more
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