“…The lack of Rvs161-Rvs167 and Bzz1 results in an improper geometry of invagination (Kishimoto et al, 2011). Similarly, deletion of VPS1, whose gene product is a dynamin-like protein, results in 'yo-yo' phenotypes and misshaped invaginations (Smaczynska-de Rooij et al, 2010), consistent with a role in regulating membrane shape and scission, potentially through an F-actin-bundling activity (Palmer et al, 2015). Unlike the conventional dynamins involved in mammalian CME, and similar to other dynamin-like proteins, Vps1 does not contain a pleckstrinhomology (PH) domain.…”