A dynamic model for intracellular calcium response in fibroblasts induced by electrical stimulation

Sun, Guoxin; Wang, Lijun; Chen, Xiang; Qin, Kairong

doi:10.1016/j.mbs.2013.04.005

Cited by 15 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9,10 At the same time, ES can activate cellular ion channels and downstream transduction signals to guide the migration and proliferation of skin cells, especially epithelial cells and fibroblasts. 11 Therefore, ES is also an effective treatment for accelerating skin regeneration.…”

Section: Introductionmentioning

confidence: 99%

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

Ren,

Zhang,

et al. 2024

IJN

View full text Add to dashboard Cite

Background: Electrical stimulation (ES) can effectively promote skin wound healing; however, single-electrode-based ES strategies are difficult to cover the entire wound area, and the effectiveness of ES is often limited by the inconsistent mechanical properties of the electrode and wound tissue. The above factors may lead to ES treatment is not ideal. Methods: A multifunctional conductive hydrogel dressing containing methacrylated gelatin (GelMA), Ti 3 C 2 and collagen binding antimicrobial peptides (V-Os) was developed to improve wound management. Ti 3 C 2 was selected as the electrode component due to its excellent electrical conductivity, the modified antimicrobial peptide V-Os could replace traditional antibiotics to suppress bacterial infections, and GelMA hydrogel was used due to its clinical applicability in wound healing. Results:The results showed that this new hydrogel dressing (GelMA@Ti 3 C 2 /V-Os) not only has excellent electrical conductivity and biocompatibility but also has a durable and efficient bactericidal effect. The modified antimicrobial peptides V-Os used were able to bind more closely to GelMA hydrogel to exert long-lasting antibacterial effects. The results of cell experiment showed that the GelMA@Ti 3 C 2 /V-Os hydrogel dressing could enhance the effect of current stimulation and significantly improve the migration, proliferation and tissue repair related genes expression of fibroblasts. In vitro experiments results showed that under ES, GelMA@Ti 3 C 2 /V-Os hydrogel dressing could promote re-epithelialization, enhance angiogenesis, mediate immune response and prevent wound infection. Conclusion: This multifunctional nanocomposite hydrogel could provide new strategies for promoting infectious wound healing.

show abstract

Section: Introductionmentioning

confidence: 99%

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

Ren,

Zhang,

et al. 2024

IJN

View full text Add to dashboard Cite

show abstract

“…On the other hand, inter-cellular Ca 2+ waves were reportedly dominated by IP 3 and Ca 2+ signaling through gap junctions and paracrine adenosine triphosphate (ATP) messaging [1]. The IICR/CICR models and one/two dimensional (1D and 2D) mathematical models of the intraand inter-cellular Ca 2+ waves in various cells have been proposed to study the metabolism and diffusion processes of Ca 2+ [5][6][7][8][9][10][11][12]. In this review, we summarize the mathematical models for the numerical simulation of intra-and intercellular Ca 2+ wave propagation.…”

Section: Introductionmentioning

confidence: 99%

Mathematical models for intra- and inter-cellular Ca<sup>2+</sup> wave propagations

Sera

Kudo

2020

J Biorheol

View full text Add to dashboard Cite

Intra-and inter-cellular Ca 2+ waves play key roles in cellular functions. Focal stimulation triggers Ca 2+ wave propagation from the stimulation point to neighboring cells through the cytoplasm, which involves localized metabolism reactions and specific diffusion processes. Briefly, inositol 1,4,5-trisphosphate (IP 3 ) is produced at membranes and diffuses into the cytoplasm, resulting in Ca 2+ release from the endoplasmic reticulum (ER). Particularly, Ca 2+ released from the ER is mediated by two principles, the IP 3 -induced Ca 2+ and Ca 2+ -induced Ca 2+ releases. Ca 2+ is diffused through the cytoplasm and, furthermore, transported into neighboring cells through gap junctions. These intra-and inter-cellular Ca 2+ waves have been widely investigated using theoretical and experimental methods in various cell types. In this review we summarize the mathematical models used for the numerical simulation of intraand inter-cellular Ca 2+ wave propagations. Keywords Numerical simulation, Ca 2+ wave, Ca 2+ -induced Ca 2+ release, IP 3 -induced Ca 2+ release

show abstract

“…Electrophysiological study reveals the presence of voltage-sensitive calcium channels in fibroblasts [11]. Levels of free Ca 2+ rise in fibroblasts under ES [12], thus causing Ca 2+ -sensitive processes such as cytoskeletal organization, cell motility, and cell growth. Our previous research indicates that ES improved the outcome of UI symptoms using stress urinary incontinence (SUI) mouse model [13], and Smads signaling may be involved in this process.…”

Section: Introductionmentioning

confidence: 99%

Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca²⁺: Potential Mechanism of Pelvic Electrical Stimulation Therapy

Tang

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Ca2+ is an important ion in response to electrical stimulation (ES) and acts as second messenger in the regulation of various physiological processes. Pelvic floor electrical stimulation (PES) is a low-voltage clinical application, available for urinary incontinence (UI) treatment. Fibroblasts, as the main cellular component of vaginal wall and pelvic ligament, play an important role in the maintenance of pelvic health. We studied the effect of ES on fibroblasts in this study. ES was conducted with electrotaxis chambers on L929 fibroblast and the ES parameter was 100 mV/mm×2h. The results showed that ES increased intracellular Ca2+ concentration, promoted the expression of PCNA, CyclinB1, and CyclinD1, and increased the proportion of cells in S and G2 phages. After ES, fibroblasts get activated and proliferated. Besides, BAPTA-AM, a membrane permeated chelator for intracellular free Ca2+, partially inhibited the effect of ES on fibroblasts activation and proliferation promotion. Furthermore, we elucidated that Ca2+, as a second messenger and upstream signal for Smads and Akt signaling, regulated ES-induced nuclear translocation of smad2/3, phosphorylation of smad2/3, Akt, and GSK3β. Finally, we validated the effect of ES on PES mouse model. The results indicated that PES promoted the activation and proliferation of fibroblasts in vivo. In conclusion, we verify that ES can elevate the concentration of intracellular Ca2+ and activate its downstream signaling and then promote the activation of fibroblasts, which may be one of the mechanisms of PES therapy.

show abstract

A dynamic model for intracellular calcium response in fibroblasts induced by electrical stimulation

Cited by 15 publications

References 26 publications

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

Mathematical models for intra- and inter-cellular Ca<sup>2+</sup> wave propagations

Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca²⁺: Potential Mechanism of Pelvic Electrical Stimulation Therapy

Contact Info

Product

Resources

About

A dynamic model for intracellular calcium response in fibroblasts induced by electrical stimulation

Cited by 15 publications

References 26 publications

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing

Mathematical models for intra- and inter-cellular Ca<sup>2+</sup> wave propagations

Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca2+: Potential Mechanism of Pelvic Electrical Stimulation Therapy

Contact Info

Product

Resources

About

Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca²⁺: Potential Mechanism of Pelvic Electrical Stimulation Therapy