A dynamic and stationary rheological study of erythrocytes incubated in a glucose medium

Riquelme, Bibiana Doris; Foresto, Patricia; D’Arrigo, Mabel; Valverde, Juana; Rasia, Rodolfo M.

doi:10.1016/j.jbbm.2004.10.004

Cited by 28 publications

(30 citation statements)

References 13 publications

(14 reference statements)

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“…Figure 4(e) shows the presence of rather large aggregated structures, confirming previous findings of the formation of larger aggregates when high concentrations of glucose are involved. 28,29 For the case of the PMMA microbeads, we have also observed the creation of organized structures within the Dx 40 medium. Overall, the performed aggregation assessment shows that the glucose-rich RBCs present more aggregation then the healthy RBCs suspended in the same medium and the PMMA microbeads present similar behavior in both media.…”

Section: -9mentioning

confidence: 77%

“…In Fig. 3, it 25,[27][28][29] In contrast, the results obtained for Region 3 show that glucose-rich RBCs have a larger DIs when compared with the healthy RBCs. This could be explained by the difficulty of the glucose-rich RBCs in recovering their original shape due to the reduction in their membrane viscoelastic properties.…”

Section: Deformability Analysismentioning

confidence: 99%

“…This approach has been successfully used for a better understanding of RBC mechanical modifications and in particular the investigation of the impairment of microcirculation in diabetic patients. 22,23,[27][28][29] In this work, we have also applied this method to obtain pathological suspensions of RBCs due to the difficulty in acquiring and handling pathological blood. Hence, it is crucial to develop blood analogues that are able to mimic the hemodynamic and hemorheological properties not only of healthy but also of the pathological blood behavior.…”

Section: Introductionmentioning

confidence: 99%

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In vitro particulate analogue fluids for experimental studies of rheological and hemorheological behavior of glucose-rich RBC suspensions

et al. 2017

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Suspensions of healthy and pathological red blood cells (RBC) flowing in microfluidic devices are frequently used to perform in vitro blood experiments for a better understanding of human microcirculation hemodynamic phenomena. This work reports the development of particulate viscoelastic analogue fluids able to mimic the rheological and hemorheological behavior of pathological RBC suspensions flowing in microfluidic systems. The pathological RBCs were obtained by an incubation of healthy RBCs at a high concentration of glucose, representing the pathological stage of hyperglycaemia in diabetic complications, and analyses of their deformability and aggregation were carried out. Overall, the developed in vitro analogue fluids were composed of a suspension of semi-rigid microbeads in a carrier viscoelastic fluid made of dextran 40 and xanthan gum. All suspensions of healthy and pathological RBCs, as well as their particulate analogue fluids, were extensively characterized in steady shear flow, as well as in small and large amplitude oscillatory shear flow. In addition, the well-known cell-free layer (CFL) phenomenon occurring in microchannels was investigated in detail to provide comparisons between healthy and pathological in vitro RBC suspensions and their corresponding analogue fluids at different volume concentrations (5% and 20%). The experimental results have shown a similar rheological behavior between the samples containing a suspension of pathological RBCs and the proposed analogue fluids. Moreover, this work shows that the particulate in vitro analogue fluids used have the ability to mimic well the CFL phenomenon occurring downstream of a microchannel contraction for pathological RBC suspensions. The proposed particulate fluids provide a more realistic behavior of the flow properties of suspended RBCs when compared with existing non-particulate blood analogues, and consequently, they are advantageous for detailed investigations of microcirculation.

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Section: -9mentioning

confidence: 77%

Section: Deformability Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro particulate analogue fluids for experimental studies of rheological and hemorheological behavior of glucose-rich RBC suspensions

et al. 2017

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“…According to a study in 2012, it was shown that even only 5 minutes exposure to hyperglycemia might change erythrocyte deformability (39). Riquelme et al (40) showed that 2 hours exposure to hyperglycemia changed viscoelastic feature of erythrocyte membrane dramatically, Shin et al showed that erythrocyte deformability was depended on dose and time as well (41). Diltoer and Camu showed that isoflurane caused glucose intolerance (42).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

2018

GMJ

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Aim: Micro and macrovascular complications due to long-term hyperglycemia are associated with increased mortality and morbidity. Erythrocytes exposed to hyperglycemia for a long time may cause morphological changes in erythrocytes such as decreased deformability and development of aggregation. As a result, complications such as shortening life span of erythrocytes, impairment of oxygen carrying capacity, tissue hypoxia may occur. In our study, we would like to investigate the effects of Sevoflurane and Desflurane on erythrocyte deformability during transient hyperglycemia. Materials and Methods: In this study, 30 male Wistar albino rats were used. The animals were randomly divided into five groups, each contained 6 rats: Diabetic control (group DC), diabetic hyperglycemia group (group DH), diabetic hyperglycemia group with desflurane (group DH-D), and diabetic hyperglycemia group with sevoflurane (group DH-S) groups. Another 6 rats without diabetes were assigned as control group (group C). Streptozotocininduced diabetic rats were kept 6 weeks, then transient hyperglycemia was created, and the administration of sevoflurane and desflurane were performed. After 24 hours blood samples were obtained and deformability measurements were performed in erythrocyte suspensions containing Htc 5% in a PBS buffer. Results: Diabetes mellitus was found to increase relative resistance in the control group (p <0.0001). Acute hyperglycemia increased relative resistance in diabetes control, relatively. Group DH, Group DH-D and Group DH-S deformability index were significantly different when compared to Group DC (p=0.007, p=0.025, p=0.016, respectively). It was found that administration of desflurane or sevoflurane did not alter erythrocyte deformability during acute hyperglycemia (p = 0.591, p = 0.739). Conclusion: As a consequence, we think that we can safely use inhalation anesthetics such as Desflurane and Sevoflurane during acute hyperglycemia attacks. But, it needs further investigation as both experimental and clinical

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“…This finding correlates with recent reports that explain how glucose exposure for hours significantly affects protein modification and inhibits RBC deformation. Riquelme et al reported that high glucose concentrations caused significant reductions in the viscoelastic properties of the erythrocyte membrane when the erythrocytes were incubated for 2 hours [44]. Shin et al found similar associations, with both deformability and erythrocyte aggregation decreasing in a doseand time-dependent manner [45].…”

Section: A Decrease In Pkc Translocation 2 a Reduction In Pkcα Bindimentioning

confidence: 99%

Effect of Short-Term Hyperglycemia on Protein Kinase C Alpha Activation in Human Erythrocytes

Livshits¹,

Srulevich²,

Cahn³

et al. 2012

Rev Diabet Stud

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■ AbstractBACKGROUND: Diabetes mellitus, characterized by chronic hyperglycemia, is known to have a deleterious effect on erythrocyte structure and hemodynamic characteristics, which eventually contribute to diabetes-associated vascular complications. Protein kinase C alpha (PKCα) is a major regulator of many metabolic processes and structural changes in erythrocytes, and may play a significant role in the development of hyperglycemia-mediated cellular abnormalities. AIM: We hypothesized that acute hyperglycemic stress may affect erythrocyte structure and metabolic properties through its effect on PKCα membrane content and activity. RESULTS: Erythrocytes, from healthy individuals acutely exposed to a glucose enriched media, showed a significant decrease in the membranous fraction of PKCα and its phosphorylation (p = 0.005 and p = 0.0004, respectively). These alterations correlated with decreased affinity of PKCα to its membrane substrates (4.1R and GLUT1) and reduced RBC deformability (p = 0.017). Pre-activation of erythrocytes with PKC activator, PMA, minimized the effect of glucose on the membrane PKCα fraction and RBC deformability (p > 0.05). CONCLUSIONS: Acute glycemia-induced inhibition of PKCα membranous translocation and activation is associated with reduced erythrocyte membrane deformability.

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A dynamic and stationary rheological study of erythrocytes incubated in a glucose medium

Cited by 28 publications

References 13 publications

In vitro particulate analogue fluids for experimental studies of rheological and hemorheological behavior of glucose-rich RBC suspensions

In vitro particulate analogue fluids for experimental studies of rheological and hemorheological behavior of glucose-rich RBC suspensions

Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

Effect of Short-Term Hyperglycemia on Protein Kinase C Alpha Activation in Human Erythrocytes

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