A dumbell probe-mediated rolling circle amplification strategy for highly sensitive transcription factor detection

Li, Chunxiang; Qiu, Xiyang; Hou, Zhaohui; Deng, Keqin

doi:10.1016/j.bios.2014.09.068

Cited by 37 publications

(16 citation statements)

References 31 publications

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“…As shown in Fig. 5 the reported works whose recoveries were from 92.5% to 107% [38,39], these results indicated that the strategy was reliable and had the potential for real sample application. …”

Section: Detection Of Nf-κb P50 In Spiked Samplesupporting

confidence: 59%

Fok I cleavage–inhibition strategy for the specific and accurate detection of transcription factors

Gao

Wang

Zhu

et al. 2015

Talanta

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Section: Detection Of Nf-κb P50 In Spiked Samplesupporting

confidence: 59%

Fok I cleavage–inhibition strategy for the specific and accurate detection of transcription factors

Gao

Wang

Zhu

et al. 2015

Talanta

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“…In recent years, some groups have noticed the special characteristics of dumbbell probe (DP) employed in RCA, and achieved exciting progress. It was used to detect RNA and transcription factor sensitively by intercalating SYBR Green I into the stem part of DP 18 19 . Combined with nickase and G-quadruplex, a DNAzyme method was proposed 20 .…”

mentioning

confidence: 99%

Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification

Hua

Tang

et al. 2016

Sci Rep

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Dumbbell probe (DP) attracts increasing interests in rolling circle amplification (RCA). A universal DP production method through cleavage-ligation of hairpin was proposed and optimized. The production is characterized by restriction endonuclease (RE)-induced cleavage ends ligation. It has the advantage of phosphorylation-free, splint-free and purification-free. To optimize designing, we found that the position of RE cleavage sequence in the stem and the primer position in the loop affected the formation and amplification of DP obviously. Both sticky and blunt ends cleaved by RE produce DP efficiently. Moreover, we introduced this DP into circle to circle (C2C) RCA based on the same cleavage-ligation principle, and acquired high sensitivity. By combining a two-ligation design and the C2C strategy, specificity for detecting let-7 family members was increased extremely. Furthermore, coreaction of different steps facilitated convenient formation and amplification process of DP.

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“…Therefore, engineering a high-efficiency immunosensor has become an emerging and powerful strategy for proteome and genomics research, also for clinical diagnosis. An efficient fluorescent-amplification method for fast, quantitative, and low cost detection of TF has been developed by Li et al [ 79 ] utilizing RCA using TBP as a target model. Three components were used in their study: TF binding probe (TFBP), enlargement primer, and dumbbell-probe.…”

Section: Transcription Factor (Tf) Biosensor Based On Rcamentioning

confidence: 99%

Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

Yan

Liu

et al. 2018

Pharmaceuticals

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Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic process that utilizes nuclease to generate long single stranded DNA (ssDNA) or RNA. The functional nucleic acid unit (aptamer, DNAzyme) could be replicated hundreds of times in a short period, and a lower LOD could be achieved if those units are combined with an enzymatic reaction, Surface Plasmon Resonance, electrochemical, or fluorescence detection, and other different kinds of biosensor. Multifarious RCA-based platforms have been developed to detect a variety of targets including DNA, RNA, SNP, proteins, pathogens, cytokines, micromolecules, and diseased cells. In this review, improvements in using the RCA technique for medical biosensors and biomedical applications were summarized and future trends in related research fields described.

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A dumbell probe-mediated rolling circle amplification strategy for highly sensitive transcription factor detection

Cited by 37 publications

References 31 publications

Fok I cleavage–inhibition strategy for the specific and accurate detection of transcription factors

Fok I cleavage–inhibition strategy for the specific and accurate detection of transcription factors

Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification

Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

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