A dual-signal self-checking photoelectrochemical immunosensor based on the sole composite of MIL-101(Cr) and CdSe quantum dots for the detection of α-fetoprotein

Zhong, Xiaolin; Zhang, Min; Guo, Lu’an; Xie, Yongze; Luo, Rong; Chen, Wenxue; Cheng, Faliang; Wang, Lishi

doi:10.1016/j.bios.2021.113389

Cited by 49 publications

(19 citation statements)

References 40 publications

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“…The AFP immunosensor can not only provide highly sensitive detection, but also provide the high accuracy and assay ability. Thus, the prepared photoelectrochemical AFP immunosensor showed a LOD of 0.054 ng mL −1 [3]. Hence, the applicability of electrochemical immunosensor technology for the high sensitivity and selectivity determination of AFP appears to be appropriate.…”

Section: Introductionmentioning

confidence: 98%

“…Hence, from the past to the present, a great deal of study in the field of cancer has been focused on developing the appropriate and early diagnosis of the disease. α-Fetoprotein (AFP), an important tumor biomarker of primary lung cancer, gonadal or extragonadal yolk sac tumor, testicular tumor, or gastric cancer, is a glycoprotein with a molecular weight of ca.70 kDa and produced by the yolk sac and liver during the early embryonic period [2][3][4]. It has been reported that the concentration of AFP, which is approximately 25.0 ng mL −1 in healthy human blood serum, reached approximately 30-folds of it in cancer patients [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

et al. 2022

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The early diagnosis of major diseases such as cancer is typically a major issue for humanity. Human α-fetoprotein (AFP) as a sialylated glycoprotein is of approximately 68 kD molecular weight and is considered to be a key biomarker, and an increase in its level indicates the presence of liver, testicular, or gastric cancer. In this study, an electrochemical AFP immunosensor based on Fe 3 O 4 NPs@covalent organic framework decorated gold nanoparticles (Fe 3 O 4 NPs@COF/AuNPs) for the electrode platform and double-coated magnetic nanoparticles (MNPs) based on SiO 2 @TiO 2 (MNPs@SiO 2 @TiO 2 ) nanocomposites for the signal amplification was fabricated. The immobilization of anti-AFP capture antibody was successfully performed on Fe 3 O 4 NPs@COF/AuNPs modified electrode surface by amino-gold affinity, while the conjugation of anti-AFP secondary antibody on MNPs@SiO 2 @TiO 2 was achieved by the electrostatic/ionic interactions. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) analysis, cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) techniques were used to characterize the nanostructures in terms of physical and electrochemical features. The limit of detection (LOD) was 3.30 fg mL −1 . The findings revealed that the proposed electrochemical AFP immunosensor can be effectively used to diagnose cancer.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

et al. 2022

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“…The sample volume required for screen printing electrodes is only tens of microliter. Fluorescence immunoassay does not require the use of electrodes and additional voltage, which can avoid the situation of unclean electrodes, unreasonable electrode preparation, and unstable applied voltage in the process of ECL operation [ 18 ]. Using fluorescence and ECL dual signals to analyze the target is conducive to mutual verification and improving the accuracy of the sensor.…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Fluorescence and Screen-Printed Electrodes—Electrochemiluminescence Immunosensor for Ricin Detection Based on CdSe/ZnS QDs with Dual Signal

Feng

Hu²,

Pei

et al. 2022

Toxins

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A sensitive dual-readout immunosensor for fluorescence and electrochemiluminescence (ECL) detection of ricin was established, which was combined with a streptavidin–biotin signal amplification system. CdSe/ZnS quantum dots with fine fluorescence and ECL properties were used as the dual-signal function probes of the sandwich immunocomplex. Under the optimum experimental conditions, the dual signal intensity increased significantly with the rise in ricin concentration. The fluorescence intensity of the senor exhibited a good liner relationship toward the ricin concentrations with 0.1~100 ng/mL and the limit of detection (LOD) was 81.7 pg/mL; taking ECL as the detection signal, the sensor showed a linear relationship with the ricin concentrations ranging from 0.01 ng/mL to 100 ng/mL and the LOD was 5.5 pg/mL. The constructed sensor with high sensitivity had been successfully applied to the detection of ricin in complex matrices with satisfactory recoveries. The proposed immunosensor model can be extended to the analysis and detection of others target proteins.

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“…Moreover, the preparation process of this biosensor was simple, and powerful detection functions could be obtained. Biosensors with multisignal outputs − can mutually verify the detection results of different signals, which will enhance the accuracy and reliability of the detection results.…”

Section: Introductionmentioning

confidence: 99%

Organic Field-Effect Transistor-Based Biosensors with Enhanced Sensitivity and Reliability under Illumination for Carcinoembryonic Antigen Bioassay

et al. 2021

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Achieving biosensors of high sensitivity and reliability is extremely significant for early diagnosis and treatment of tumor diseases. Herein, a novel organic field-effect transistor (OFET)-based biosensor was developed and applied for carcinoembryonic antigen (CEA) bioassay. This OFET-based biosensor can respond sensitively to the antigen−antibody immune-recognition reaction under illumination and darkness, respectively, thereby generating electrical signal changes of source-drain current (I DS ) and threshold voltage (V th ). The OFET-based biosensor exhibits detection limits for CEA detection of 0.5 and 0.2 pM, respectively, using I DS and V th as the response signals under darkness. When a specific intensity of light is applied, light will influence the charge-carrier transport process in the conductive channel, thus causing biosignals to turn into higher electrical signal changes of photocurrent and threshold voltage under illumination. Compared with the detection results in the dark, the biosensor exhibits higher sensitivity for CEA detection under illumination with detection limits of 13.5 and 16.9 fM. Also, multisignal outputs effectively improve the reliability of the biosensor for CEA detection. Consequently, with powerful detection functions, this OFET-based biosensor is expected to become a high-performance biosensing platform for the detection of various biological substances in the future.

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A dual-signal self-checking photoelectrochemical immunosensor based on the sole composite of MIL-101(Cr) and CdSe quantum dots for the detection of α-fetoprotein

Cited by 49 publications

References 40 publications

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

A Highly Sensitive Fluorescence and Screen-Printed Electrodes—Electrochemiluminescence Immunosensor for Ricin Detection Based on CdSe/ZnS QDs with Dual Signal

Organic Field-Effect Transistor-Based Biosensors with Enhanced Sensitivity and Reliability under Illumination for Carcinoembryonic Antigen Bioassay

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