A dual-signal colorimetric and near-infrared fluorescence probe for the detection of exogenous and endogenous hydrogen peroxide in living cells

The role of reactive oxygen species in tumor treatment

“…[43][44][45] Alternatively, DHE can react with O 2 À to produce another uorescent product, which then generates uorescence while chimeric with DNA. 42,43 Some of the nanocomposites used in cancer therapy, combined with PDT, can also produce ROS under the irradiation of external light source, so the corresponding probes can be used to detect ROS production. 1,3-Diphenylisobenzofuran (DPBF) can be oxidized by ROS (mainly O 2…”

A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging

“…With the increase of hydrogen peroxide concentration, the fluorescence intensity of CMB increased by about 25-fold ( Figure 1 c). There is a good linear relationship between the fluorescence intensity and the concentration of H 2 O 2 in the range of 0 to 50 μM with a correlation coefficient of 0.9979 ( Figure 1 d) and the detection limit was 0.13 μM, thus being able to be used for direct imaging of physiological H 2 O 2 [ 23 ]. This fluorescence change may be attributed to the structural change from CMB to CM caused by the reaction with H 2 O 2 .…”

“…It tends to be used for detecting and tracking certain biomolecules in cells or organisms [ 19 ] because of its non-invasiveness and ease of operation [ 20 , 21 , 22 ], and thus has great potential in detecting small molecules’ markers to help in the diagnosis of some diseases. Some fluorescent small-molecule probes for detecting ROS have been reported [ 23 , 24 , 25 , 26 , 27 ]. These studies proved the feasibility of using fluorescent small-molecule probes to detect H 2 O 2 and can promote the development and progress of H 2 O 2 probes, which may facilitate the realization of its applications in medicine.…”

An ICT-based fluorescence enhancement probe for detection of Sn2+ in cancer cells

“…16 Compare with these analytical approaches, uorescent sensors have been developed for the determination of various important chemical species, as they offer signicant advantages of excellent sensitivity, simplicity, instantaneous response and low detection limit up to nanomolar scale by contrast with routine analysis. [17][18][19] Therefore, the study of uorescence probe as the high selectivity and sensitivity detection of Sn 2+ is urgently desirable. Hitherto many of uorescence probes for the detection of Sn 2+ , which sensing mechanisms are mainly based on chemical reaction, complexation reaction, quantum dots, and nanoparticles, have been established.…”