A Dual Role for Intracellular Trehalose in the Resistance of Yeast Cells to Water Stress

Sano, Fumihiko; Asakawa, Naoki; Inoue, Yoshio; Sakurai, Minoru

doi:10.1006/cryo.1999.2188

Cited by 77 publications

(47 citation statements)

References 27 publications

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“…These compatible organic solutes are generally favored over the accumulation of inorganic ions because they limit the perturbation of macromolecules even at high concentrations (Yancey, 2001). In addition to their colligative effect in reducing the gradient for water loss, many organic solutes also have other physiological properties, such as stabilizing membranes and proteins during cell shrinkage (Crowe et al, 1984;Crowe et al, 1992;Sano et al, 1999), which preserve cellular function. Among arthropods, larvae of the euryhaline mosquito C. tarsalis accumulate high concentrations of proline and trehalose in response to increased environmental salinity (Garrett and Bradley, 1987;Patrick and Bradley, 2000).…”

Section: Tolerance and Physiological Response To Salinitymentioning

confidence: 99%

Osmoregulation and salinity tolerance in the Antarctic midge,Belgica antarctica: seawater exposure confers enhanced tolerance to freezing and dehydration

Elnitsky

Benoit

López‐Martínez

et al. 2009

Journal of Experimental Biology

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SUMMARYSummer storms along the Antarctic Peninsula can cause microhabitats of the terrestrial midge Belgica antarctica to become periodically inundated with seawater from tidal spray. As microhabitats dry, larvae may be exposed to increasing concentrations of seawater. Alternatively, as a result of melting snow or following rain, larvae may be immersed in freshwater for extended periods. The present study assessed the tolerance and physiological response of B. antarctica larvae to salinity exposure, and examined the effect of seawater acclimation on their subsequent tolerance of freezing, dehydration and heat shock. Midge larvae tolerated extended exposure to hyperosmotic seawater; nearly 50% of larvae survived a 10-day exposure to 1000 mOsm kg -1 seawater and ~25% of larvae survived 6 days in 2000 mOsm kg -1 seawater. Exposure to seawater drastically reduced larval body water content and increased hemolymph osmolality. By contrast, immersion in freshwater did not affect water content or hemolymph osmolality. Hyperosmotic seawater exposure, and the accompanying osmotic dehydration, resulted in a significant correlation between the rate of oxygen consumption and larval water content and induced the de novo synthesis and accumulation of several organic osmolytes. A 3-day exposure of larvae to hyperosmotic seawater increased freezing tolerance relative to freshwater-acclimated larvae. Even after rehydration, the freezing survival of larvae acclimated to seawater was greater than freshwater-acclimated larvae. Additionally, seawater exposure increased the subsequent tolerance of larvae to dehydration. Our results further illustrate the similarities between these related, yet distinct, forms of osmotic stress and add to the suite of physiological responses used by larvae to enhance survival in the harsh and unpredictable Antarctic environment.

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Section: Tolerance and Physiological Response To Salinitymentioning

confidence: 99%

Osmoregulation and salinity tolerance in the Antarctic midge,Belgica antarctica: seawater exposure confers enhanced tolerance to freezing and dehydration

Elnitsky

Benoit

López‐Martínez

et al. 2009

Journal of Experimental Biology

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“…Specifically, trehalose is found in numerous organisms, such as nematodes and yeasts, which are capable of surviving during freezing and drying (8,9). Previous studies have used this disaccharide for cryopreservation of human cells, such as platelets (10), red blood cells (11), sperm (12), oocytes (13), pancreatic islets (14) and fetal skin (15).…”

Section: Introductionmentioning

confidence: 99%

Effect of trehalose on cryopreservation of pure peripheral blood stem cells

Martinetti¹,

Colarossi²,

Buccheri³

et al. 2017

Biomedical Reports

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Abstract. Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post-thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thawing. Thus, the use of cryoprotectants is essential. In particular, the efficacy of trehalose has been reported for clinical purposes in blood stem cells. The aim of the current study was to establish an efficient method for biological research based on the use of trehalose, to cryopreserve pure peripheral blood stem cells. The efficacy of trehalose was assessed in vitro and the cell viability was evaluated. The data indicate that trehalose improves cell survival after thawing compared with the standard freezing procedure. These findings could suggest the potential for future trehalose application for research purposes in cell cryopreservation. IntroductionStem cells in biological research provide a significant source of information and, thus, contribute to the development of novel therapeutic strategies. In particular, the ability to cultivate stem cells and human hematopoietic progenitor cells in vitro is the fundamental basis for investigating hematopoiesis. An improved understanding of the mechanisms that regulate cell proliferation and differentiation during the stages of hematopoiesis would further elucidate the molecular characteristics of diseases (which are characterized by excessive expansion or a functional defect of certain immature blood components), and facilitate the identification of substances that are able to specifically protect healthy cells from the action of cytotoxic drugs.Hematopoietic stem and progenitor cells (HSPCs) may be isolated from peripheral blood (PB), bone marrow (BM) or umbilical cord blood (CB) (1).Although significant improvements to cell cryopreservation procedures have been achieved (2), the improvement of current cryopreservation protocols that lead to a high mortality rate after thawing for the crystal formations that arise during freezing is a primary goal. Specifically, fast cooling forms intracellular ice crystals, which results in cell destruction and slow cooling forms ice crystals in the extracellular space, with consequent cellular dehydration. Selection of a cryoprotectant, as well as a suitable freezing rate serves to protect cells from these adverse effects (3,4).Cryoprotectants are divided into two classes: Penetrating and nonpenetrating (5). The penetrating cryoprotectants include glycerol and 1,2-propanediol and dimethyl sulfoxide (Me 2 SO); the latter is commonly used for HSPC cryopreservation (6).The non penetrating cryoprotectants comprise polyvinyl pyrrolidone, trehalose, fructose, sucrose and glucose.Trehalose is a non-toxic disaccharide of glucose that preserves the structural integrity of the cells during freezing and thawing (7). Specifically, trehalose is found in numerous organisms, such as nematodes and yeasts, which are capable of surviving du...

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“…Moreover, it modifies the temperature at which the separation of lipid phase occurs during cooling (Crowe et al, 1984;Crowe et al, 1985;Crowe et al, 2001). As compared to other sugars, trehalose seems to have a higher capacity to preserve biomolecules, cellular membrane and cells in a drying or in a freezing state (Crowe et al, 1996;Storey et al, 1998;Sano et al, 1999;Welsh and Herbert, 1999).…”

Section: Discussionmentioning

confidence: 99%