A dual-response fluorescent probe for the discrimination of cysteine from glutathione and homocysteine

BODIPY‐Based Fluorescent Probes for Biothiols

“…We demonstrated that the spatial steric hindrance of the methyl groups at the 1-and 7-positions could be exploited to differentiate the slight differencei nt he steric repulsion of Cys and Hcy.B oth probesc ould be used to image Cys and GSH synchronously in living HeLa cells by dual disparate emission channels. When para-nitrophenyl carboxyl-ate was placed in the meso position of BODIPY scaffold 84 (Figure 20), [114] the same detection resultsw ere obtained.…”

Fluorescent probes based on nucleophilic aromatic substitution reactions for reactive sulfur and selenium species: Recent progress, applications, and design strategies

“…Therefore, NBD-O ether can be used to distinguish RSS with different structures but similar p K a values [210] , [211] . Thiolysis of the NBD-N group is H 2 S-specific, and may be exploited for the development of highly selective fluorescent probes and scavengers for H 2 S. The active sites of benzoates mainly simulate NCL reaction [221] , [222] . In fact, regardless of whether the detection site is thiobenzoate or benzoate, the test substance always forms a new thiolipid with the carboxyl group.…”

“…In fact, regardless of whether the detection site is thiobenzoate or benzoate, the test substance always forms a new thiolipid with the carboxyl group. For benzoate esters, the exposure of hydroxyl groups of the fluorophore occurs concomitantly with the combination of RSS and benzoic acid [221] , [229] . For the active sites of thiobenzoates, due to the powerful quenching effect of sulfur atoms, the thiophenol-containing groups are always displaced, and the fluorophores combine with the analyte to produce fluorescence at different emission wavelengths.…”

“…At the same time, the probe is quenched by the quenching group. The associated fluorescence changes provide the detection signal [221] , [222] , [223] , [224] , [225] , [226] , [227] , [228] .…”

Selective detection of glutathione based on the recovered fluorescence of BSA‐AuNCs/Cu 2+ system

“…Up to now, many fluorescent probes and sensors have been reported for the detection of GSH, including organic fluorophores [13], nanocomposite [14, 15] and quantum dots (QDs) [16, 17], but many of them need complex synthetic strategies and operation, expensive reagents consumption and time‐consuming. Therefore, the choice of fluorescent sensors with favourable fluorescence properties in a high selective, simple preparation, low cost, and easy operations, is the key issue.…”