A dual PMMA/calcium sulfate carrier of vancomycin is more effective than PMMA‐vancomycin at inhibiting <i>Staphylococcus aureus</i> growth <i>in vitro</i>

Luo, Shanchao; Jiang, Tongmeng; Long, Lina; Yang, Yingnian; Yang, Xiaoping; Luo, Lan; Li, Jinli; Chen, Zhiyu; Zou, Chongqi; Luo, Sulan

doi:10.1002/2211-5463.12809

Cited by 9 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the extracts of UMAOH CaP carriers with VMN can inhibit bacteria growth more effectively in vitro as compared to pure VMN drug (Table 2, Figure 12). A similar potentiating effect of calcium salts in VMN-loaded calcium-based carriers has been found to inhibit the growth of S. aureus [61] and methicillin-resistant S. aureus [82].…”

Section: Antibacterial Studiessupporting

confidence: 52%

“…CaP degradation contributes significantly to the release of adsorbed molecules [60]. There are the absorbable properties and gradual degradation characteristics of calcium salts [61]. In this regard, it can be assumed that the established "wave-like" kinetics of IFNα release are due to the change in the processes of desorption from the surface of the samples and precipitation of biological molecules from the solution onto the UMAOH CaP coating, as was previously described for calcium ions and inorganic phosphorus during its in vitro degradation.…”

Section: Drugs Immobilization and Desorption Kineticsmentioning

confidence: 99%

See 1 more Smart Citation

UMAOH Calcium Phosphate Coatings Designed for Drug Delivery: Vancomycin, 5-Fluorouracil, Interferon α-2b Case

et al. 2022

View full text Add to dashboard Cite

Drug delivery systems based on calcium phosphate (CaP) coatings have been recently recognized as beneficial drug delivery systems in complex cases of bone diseases for admission of drugs in the localized area, simultaneously inducing osteoinduction because of the bioavailable Ca and P ions. However, micro-arc oxidation (MAO) deposition of CaP does not allow for the formation of a coating with sufficient interconnected porosity for drug delivery purposes. Here, we report on the method to deposit CaP-based coatings using a new hybrid ultrasound-assisted MAO (UMAOH) method for deposition of coatings for drug delivery that could carry various types of drugs, such as cytostatic, antibacterial, or immunomodulatory compositions. Application of UMAOH resulted in coatings with an Ra roughness equal to 3.5 µm, a thickness of 50–55 µm, and a combination of high values of internal and surface porosity, 39 and 28%, respectively. The coating is represented by the monetite phase that is distributed in the matrix of amorphous CaP. Optimal conditions of coating deposition have been determined and used for drug delivery by impregnation with Vancomycin, 5-Fluorouracil, and Interferon-α-2b. Cytotoxicity and antimicrobial activity of the manufactured drug-carrying coatings have been studied using the three different cell lines and methicillin-resistant S. aureus.

show abstract

Section: Antibacterial Studiessupporting

confidence: 52%

Section: Drugs Immobilization and Desorption Kineticsmentioning

confidence: 99%

UMAOH Calcium Phosphate Coatings Designed for Drug Delivery: Vancomycin, 5-Fluorouracil, Interferon α-2b Case

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Biofilms are a major obstacle preventing the drug from destroying the infection, with the administration of systemic antibiotics resulting in inadequate drug levels at the site of infection or causing systemic toxicity. In addition, local-high drug levels can eradicate biofilm-forming organisms [ 26 27 28 29 ]. The choice of antibiotic used in the preparation of antibiotic beads should consider the impact of the drug susceptibility on pathogens.…”

Section: Discussionmentioning

confidence: 99%

Elution profiles of metronidazole from calcium sulfate beads

Ithisariyanont,

Poapolathep,

Poapolathep

et al. 2023

J Vet Sci

View full text Add to dashboard Cite

Background Antibiotic beads are used to treat local bacterial infections by delivering high drug concentrations to infected tissue. Objectives This study examined the elution characteristics of metronidazole from metronidazole-calcium sulfate (MCa) and metronidazole-calcium-potassium sulfate (MCaK) beads over 20 days and the antibacterial efficacy of the beads after storage. Methods The MCa and MCaK beads were prepared by mixing 250 mg of metronidazole and 10 g of calcium sulfate hemihydrate with water and a 3% potassium sulfate solution, respectively. The beads were placed in phosphate-buffered saline for the elution study. The metronidazole eluents were determined using high-performance liquid chromatography. The microstructures were examined by scanning electron microscopy (SEM), and the antimicrobial activity was evaluated by a microbioassay. Results For the 20-day study, the total amount of metronidazole released was greater in the MCa beads than in the MCaK beads by 6.61 ± 0.48 mg (89.11% ± 3.04%) and 4.65 ± 0.36 mg (73.11% ± 4.38%), respectively. The amounts of eluted drugs from the MCa and MCaK beads were higher than the minimum inhibitory concentration at 0.5 µg/mL against anaerobic bacteria at both 20 days and 14 days. SEM showed that calcium crystals on the outer surface had dissolved after elution, and thinner calcium crystals were prominent in the MCaK beads. The MCa and MCaK beads exhibited antibacterial activity after setting, followed by storage at room temperature or 4°C for 21 days. Conclusions The MCa beads could release more drug than the MCaK beads, but all eluted metronidazole amounts were effective in controlling bacterial infections. Both metronidazole beads could be stored at ambient temperature or in a refrigerator.

show abstract

“…23,57 Polymethylmethacrylate (PMMA) is the skeleton of the hydrophobic end and is often used for osteomyelitis, bone cement, antibiotic delivery, and other associated orthopedic diseases, leading to low toxicity. [58][59][60][61] RGDfK-PHPMA-b-Poly (MMA-alt-(Rhob-MA)) (RPPMMRA) is hydrophilic at one end and hydrophobic at the other and can self-assemble into spherical vesicles encapsulating BHP in water. This vector has potential for use in cancer visualization therapy because of the presence of covalently linked Rhob-MA and RGDfK.…”

Section: Introductionmentioning

confidence: 99%

Engineered Polymeric Nanovector for Intracellular Peptide Delivery in Antitumor Therapy

Zhang,

Huang

et al. 2023

IJN

View full text Add to dashboard Cite

This study aimed to deliver a polypeptide from the Bax-BH3 domain (BHP) through the synthesis of self-assembled amphiphile nanovectors (NVs) and to assess their potential for cancer therapeutic applications and biological safety in vitro and in vivo. These findings provide valuable options for cancer intervention and a novel approach for the rational design of therapeutics. Methods: We studied the antitumor activity of BHP by preparing RGDfK-PHPMA-b-Poly (MMA-alt-(Rhob-MA)) (RPPMMRA) and encapsulating it in BHP-NV. We also performed a series of characterizations and property analyses of RPPMMRA, including its size, stability, and drug-carrying capacity. The biocompatibility of RPPMMRA was evaluated in terms of cytotoxicity and hemolytic effects. The pro-apoptotic capacity of BHP was evaluated in vitro using mitochondrial membrane potential, flow cytometry, and apoptosis visualization techniques. The potential therapeutic effects of BHP on tumors were explored using reverse molecular docking. We also investigated the in vivo proapoptotic effect of BHP-NV in a nude mouse tumor model. Results: NVs were successfully prepared with hydrated particle sizes ranging from 189.6 nm to 256.6 nm, spherical overall, and were able to remain stable in different media for 72 h with drug loading up to 15.2%. The NVs were be successfully internalized within 6 h with good biocompatibility. Neither BHP nor NV showed significant toxicity when administered alone, however, BHP-NV demonstrated significant side effects in vitro and in vivo. The apoptosis rate increased significantly from 14.13% to 66.34%. Experiments in vivo showed that BHP-NV exhibited significant apoptotic and tumor-suppressive effects. Conclusion: A targeted fluorescent NV with high drug delivery efficiency and sustained release protected the active center of BHP, constituting BHP-NV for targeted delivery. RPPMMRA demonstrated excellent biocompatibility, stability, and drug loading ability, whereas and BHP-NV demonstrated potent antitumor effects in vivo and in vitro.

show abstract

A dual PMMA/calcium sulfate carrier of vancomycin is more effective than PMMA‐vancomycin at inhibiting Staphylococcus aureus growth in vitro

Cited by 9 publications

References 38 publications

UMAOH Calcium Phosphate Coatings Designed for Drug Delivery: Vancomycin, 5-Fluorouracil, Interferon α-2b Case

UMAOH Calcium Phosphate Coatings Designed for Drug Delivery: Vancomycin, 5-Fluorouracil, Interferon α-2b Case

Elution profiles of metronidazole from calcium sulfate beads

Engineered Polymeric Nanovector for Intracellular Peptide Delivery in Antitumor Therapy

Contact Info

Product

Resources

About