A dual-model “on-super off” photoelectrochemical/ratiometric electrochemical biosensor for ultrasensitive and accurate detection of microRNA-224

Yang, Ruirui; Jiang, Guihua; Liu, Huimin; He, Leiliang; Yu, Fei; Liu, Lie; Qu, Lingbo; Wu, Yongjun

doi:10.1016/j.bios.2021.113337

Cited by 53 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, the potential of the valence band (VB) and conduction band (CB) of prepared Cu-doped ZnS was estimated by VB-XPS (Figure S9). Based on the above results and previous efforts, − possible electron transfer paths were proposed to explain the PEC response enhancing and switching on the substrate material (Figure A–C). As for the MB/Cu-doped ZnS/Au platform, the photogenerated electrons from the lowest unoccupied molecular orbital (LUMO) of MB were transferred to the CB of Cu-doped ZnS via Au NPs and further injected into the FTO electrode, resulting in the enhanced photocurrent.…”

Section: Resultsmentioning

confidence: 63%

Photocurrent-Polarity-Switching Photoelectrochemical Biosensor for Switching Spatial Distance Electroactive Tags

Zeng

et al. 2023

ACS Sens.

View full text Add to dashboard Cite

This work presents a photocurrent-polarity-switching-based photoelectrochemical (PEC) biosensing platform for ultrasensitive detection of microRNA-21 (miR-21) through target-triggered catalytic hairpin assembly (CHA) for modulation of methylene blue (MB) and ferrocene (Fc) positional configurations using double-shelled Cu-doped ZnS nanocages (NCs)–Au nanoparticles (NPs) as photoactive materials. In the presence of miR-21, the assembly of MB-labeled HP1 and Fc-labeled HP2 leads to the generation of a large amount of double-stranded DNA (HP1–HP2), which pushes MB away from the electrode surface and brings Fc close to the electrode surface, resulting in effectively quenching the enhanced PEC signal to activate the photocurrent-polarity-switching system. Benefiting from the distance-controllable strategy, the designed PEC bioanalysis can effectively eliminate false-positive and false-negative signals due to the change of different signal expression patterns (from traditional the “signal-on” mode to the photocurrent-polarity-switching mode), thereby significantly improving the sensing specificity and sensitivity. The proposed PEC sensing system exhibited satisfying photocurrent responses toward target miR-21 within the working range from 1.0 fM to 1 nM at a low limit of detection (LOD) of 0.58 fM. More importantly, we demonstrated the successful integration of the proposed PEC biosensor with a handheld wireless device for instant detection of miR-21 concentrations in practical samples.

show abstract

Section: Resultsmentioning

confidence: 63%

Photocurrent-Polarity-Switching Photoelectrochemical Biosensor for Switching Spatial Distance Electroactive Tags

Zeng

et al. 2023

ACS Sens.

View full text Add to dashboard Cite

show abstract

“…Several studies have reported that miR-224 as a potential biomarker played a vital role in HCC diagnosis and prognosis. − Next, the clinical application of this sensor was assessed in both healthy individuals and cancer patients, and the miR-224 levels were calculated from the standard curves of SERS/FL signals. As shown in Figure D, in comparison with the normal group, there was a significant elevation in miR-224 levels in HCC patients based on the data recorded by both SERS and FL methods.…”

Section: Resultsmentioning

confidence: 99%

Well-Ordered Au Nanoarray for Sensitive and Reproducible Detection of Hepatocellular Carcinoma-Associated miRNA via CHA-Assisted SERS/Fluorescence Dual-Mode Sensing

et al. 2023

View full text Add to dashboard Cite

Ultra-sensitive detection of cancer-related biomarkers in serum is of great significance for early diagnosis, treatment, prognosis, and staging of cancer. In this work, we proposed a surface-enhanced Raman scattering and fluorescence (SERS/FL) dual-mode biosensor for hepatocellular carcinoma (HCC)-related miRNA (miR-224) detection using the composition of wellarranged Au nanoarrays (Au NAs) substrate coupled with the target-catalyzed hairpin assembly (CHA) strategy. The hot spots densely and uniformly distributed on the Au array offers considerably enhanced and reproducible SERS signals, along with their wide and open surface to facilitate miR-224 adsorption. By this sensing strategy, the target miR-224 can be detected in a wide linear range (1 fM to 1 nM) with a limit of detection of 0.34 fM in the SERS mode and 0.39 fM in the FL mode. Meanwhile, this biosensor with exceptional specificity and anti-interference ability can discriminate target miR-224 from other interference miRNAs. Practical analysis of human blood samples also demonstrated considerable reliability and repeatability of our developed strategy. Furthermore, this biosensor can distinguish HCC cancer subjects from normal ones and monitor HCC patients before and after hepatectomy as well as guide the distinct Barcelona clinic liver cancer (BCLC) stages. Overall, benefiting from a well-arranged Au nanoarray, CHA amplification strategy, and SERS/metal enhanced fluorescence effect, this established biosensor opens new avenues for the early prediction, warning, monitoring, and staging of HCC.

show abstract

“…PEC biosensors are constructed based on the principle of photoelectrochemistry. Compared with traditional electrochemical analysis methods, PEC analysis technology shows lower background signal and higher sensitivity [63][64][65]. In addition, PEC biosensors do not require complex instruments and equipment, are low in cost, are easy to miniaturize, and can be used for the highly sensitive detection of life-related substances such as proteins, nucleic acid molecules, cells, small molecules, and ions [66][67][68][69].…”

Section: Photoelectrochemical Biosensormentioning

confidence: 99%

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Zhang

Gan

et al. 2023

Sensors

View full text Add to dashboard Cite

Tumor markers are important substances for assessing cancer development. In recent years, RNA tumor markers have attracted significant attention, and studies have shown that their abnormal expression of post-transcriptional regulatory genes is associated with tumor progression. Therefore, RNA tumor markers are considered as potential targets in clinical diagnosis and prognosis. Many studies show that biosensors have good application prospects in the field of medical diagnosis. The application of biosensors in RNA tumor markers is developing rapidly. These sensors have the advantages of high sensitivity, excellent selectivity, and convenience. However, the detection abundance of RNA tumor markers is low. In order to improve the detection sensitivity, researchers have developed a variety of signal amplification strategies to enhance the detection signal. In this review, after a brief introduction of the sensing principles and designs of different biosensing platforms, we will summarize the latest research progress of electrochemical, photoelectrochemical, and fluorescent biosensors based on signal amplification strategies for detecting RNA tumor markers. This review provides a high sensitivity and good selectivity sensing platform for early-stage cancer research. It provides a new idea for the development of accurate, sensitive, and convenient biological analysis in the future, which can be used for the early diagnosis and monitoring of cancer and contribute to the reduction in the mortality rate.

show abstract

A dual-model “on-super off” photoelectrochemical/ratiometric electrochemical biosensor for ultrasensitive and accurate detection of microRNA-224

Cited by 53 publications

References 39 publications

Photocurrent-Polarity-Switching Photoelectrochemical Biosensor for Switching Spatial Distance Electroactive Tags

Photocurrent-Polarity-Switching Photoelectrochemical Biosensor for Switching Spatial Distance Electroactive Tags

Well-Ordered Au Nanoarray for Sensitive and Reproducible Detection of Hepatocellular Carcinoma-Associated miRNA via CHA-Assisted SERS/Fluorescence Dual-Mode Sensing

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Contact Info

Product

Resources

About