A Dual Embryonic Origin for Vertebrate Mechanoreceptors

Collazo, Andrés; Fraser, Scott E.; Mabee, Paula M.

doi:10.1126/science.8153631

Cited by 125 publications

(83 citation statements)

References 23 publications

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“…To assess whether this phenotype is correlated with a lateral line defect, injected larvae at 72hpf were incubated with Di-Asp, a vital stain that is taken up by mature hair cells (Collazo et al, 1994;Sarrazin et al, 2006). The embryos are also labeled for alkaline phosphatase activity .…”

Section: Whole-mount Immunofluorescence Analysismentioning

confidence: 99%

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Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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Pro-protein convertase subtilisin/kexin 5 (PC5, also known as PC6) is a member of the subtilisin-like superfamily of serine proteases implicated in the maturation of latent precursor proteins into their functionally active derivatives. To investigate the functional roles, we have cloned the cDNA sequences encoding two candidate zebrafish PC5 convertases (designated as PCSK5.1 and PCSK5.2) co-orthologous to the single PC5 encoding gene (PCSK5) found in mammals. Both display syntenic correspondence to the human PCSK5 gene. Overall gene architecture has been conserved across species. While PC5.1 mRNA expression is very discrete within the otic vesicle and lateral line neuromasts, PC5.2 transcripts are more ubiquitously expressed within the central nervous system together with specific localization in various organs including liver, intestine, and otic vesicle. Zebrafish PC5.1-deficient embryos display abnormal neuromast deposition within the lateral line system and lack a normal touch response, consistent with the known sensory role that the lateral line plays in spatial awareness and sensing the environment.

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Section: Whole-mount Immunofluorescence Analysismentioning

confidence: 99%

“…When evaluating the effects of gene perturbation, Di-Aspstained neuromasts were counted on both sides of the larvae (Collazo et al, 1994;Sarrazin et al, 2006). For labeling neuromasts with alkaline phosphatase (Gilmour et al, 2004), embryos were fixed in PBS-4%PFA for 1 hr at room temperature and rinsed several times in PBS.…”

Section: Neuromast Labellingmentioning

confidence: 99%

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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“…Tuna embryos were simultaneously labeled for actin by phalloidin labeling and for acetylated tubulin by immunolabeling, as described previously (37). Neuromast hair cells were labeled by 4-(4-diethylaminostyryl)-N-methylpyridinium iodide (DiAsp, Sigma) as described previously (38). Imaging was done on a Leica SPE, a Zeiss LSM700 confocal microscope, or a Zeiss Axioimager equipped with a Coolsnap camera.…”

Section: Methodsmentioning

confidence: 99%

Innervation is required for sense organ development in the lateral line system of adult zebrafish

Wada

Dambly‐Chaudière

Kawakami

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Superficial mechanosensory organs (neuromasts) distributed over the head and body of fishes and amphibians form the "lateral line" system. During zebrafish adulthood, each neuromast of the body (posterior lateral line system, or PLL) produces "accessory" neuromasts that remain tightly clustered, thereby increasing the total number of PLL neuromasts by a factor of more than 10. This expansion is achieved by a budding process and is accompanied by branches of the afferent nerve that innervates the founder neuromast. Here we show that innervation is essential for the budding process, in complete contrast with the development of the embryonic PLL, where innervation is entirely dispensable. To obtain insight into the molecular mechanisms that underlie the budding process, we focused on the terminal system that develops at the posterior tip of the body and on the caudal fin. In this subset of PLL neuromasts, bud neuromasts form in a reproducible sequence over a few days, much faster than for other PLL neuromasts. We show that wingless/int (Wnt) signaling takes place during, and is required for, the budding process. We also show that the Wnt activator R-spondin is expressed by the axons that innervate budding neuromasts. We propose that the axon triggers Wnt signaling, which itself is involved in the proliferative phase that leads to bud formation. Finally, we show that innervation is required not only for budding, but also for long-term maintenance of all PLL neuromasts.Lgr | post-embryonic development | Thunnus thynnus | Danio rerio

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“…This technique was applied in numerous zebra-fish cell fate studies (Collazo et al, 1994;Devoto et al, 1996;Raible et al, 1992). In the context of visual system development, iontophoretic cell labeling was used to determine the developmental origins of the optic primordium (Woo and Fraser, 1995) and later to study cell rearrangements that accompany optic cup morphogenesis (Li et al, 2000b).…”

Section: Analysis Of Cell Movements and Lineage Relationshipsmentioning

confidence: 99%