A double-stranded RNA mycovirus confers hypovirulence-associated traits to<i>Botrytis cinerea</i>

Castro, Miguel E.; Kramer, Kurt; Valdivia, Lorena; Ortíz, Sylvia; Castillo, Antonio del

doi:10.1016/s0378-1097(03)00755-9

Cited by 94 publications

(62 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Depressed production of conidia has been observed for hypovirus-infected strains of C. parasitica (51) and mycovirus-infected strains of Diaporthe ambigua (52). In addition, previous studies indicated that conidium production was reduced significantly in mycovirus-associated, hypovirulent strains of B. cinerea (53,54). Unexpectedly, we found that although wild-type strain KY-1 produced conidia in a normal fashion, SsPV1-infected strains KY-1V1 and KY-1V2 of B. cinerea produced more conidia than did strain KY-1.…”

Section: Discussionmentioning

confidence: 93%

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

Xiao

Chéng

Tang

et al. 2014

J Virol

122

View full text Add to dashboard Cite

Members of the family Partitiviridae have bisegmented double-stranded RNA (dsRNA) genomes and are not generally known to cause obvious symptoms in their natural hosts. An unusual partitivirus, Sclerotinia sclerotiorum partitivirus 1 (SsPV1/WF-1), conferred hypovirulence on its natural plant-pathogenic fungal host, Sclerotinia sclerotiorum strain WF-1. Cellular organelles, including mitochondria, were severely damaged. Hypovirulence and associated traits of strain WF-1 and SsPV1/WF-1 were readily cotransmitted horizontally via hyphal contact to different vegetative compatibility groups of S. sclerotiorum and interspecifically to Sclerotinia nivalis and Sclerotinia minor. S. sclerotiorum strain 1980 transfected with purified SsPV1/WF-1 virions also exhibited hypovirulence and associated traits similar to those of strain WF-1. Moreover, introduction of purified SsPV1/WF-1 virions into strain KY-1 of Botrytis cinerea also resulted in reductions in virulence and mycelial growth and, unexpectedly, enhanced conidial production. However, virus infection suppressed hyphal growth of most germinating conidia of B. cinerea and was eventually lethal to infected hyphae, since very few new colonies could develop following germ tube formation. Taken together, our results support the conclusion that SsPV1/WF-1 causes hypovirulence in Sclerotinia spp. and B. cinerea. Cryo-EM (cryo-electron microscopy) reconstruction of the SsPV1 particle shows that it has a distinct structure with similarity to the closely related partitiviruses Fusarium poae virus 1 and Penicillium stoloniferum virus F. These findings provide new insights into partitivirus biological activities and clues about molecular interactions between partitiviruses and their hosts. IMPORTANCEMembers of the Partitiviridae are believed to occur commonly in their phytopathogenic fungal and plant hosts. However, most partitiviruses examined so far appear to be associated with latent infections. Here we report a partitivirus, SsPV1/WF-1, that was isolated from a hypovirulent strain of Sclerotinia sclerotiorum and describe its biological and molecular features. We have demonstrated that SsPV1 confers hypovirulence. Furthermore, SsPV1 can infect and cause hypovirulence in Botrytis cinerea. Our study also suggests that SsPV1 has a vigorous ability to proliferate and spread via hyphal contact. SsPV1 can overcome vegetative incompatibility barriers and can be transmitted horizontally among different vegetative compatibility groups of S. sclerotiorum, even interspecifically. Cryo-EM reconstruction of SsPV1 shows that it has a distinct structure with similarity to closely related partitiviruses. Our studies exploit a novel system, SsPV1 and its hosts, which can provide the means to explore the mechanisms by which partitiviruses interact with their hosts.

show abstract

Section: Discussionmentioning

confidence: 93%

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

Xiao

Chéng

Tang

et al. 2014

J Virol

122

View full text Add to dashboard Cite

show abstract

“…1A). A reduction in the mycelial growth of different pathogens infested with mycoviruses has been reported to be related to hypovirulence in fungi in several studies (Bottacin et al, 1994;Ahn & Lee, 2001;Castro et al, 2003;Robin et al, 2010;Rodríguez-García et al, 2014;Zheng et al, 2014;Yu et al, 2015). Nevertheless, the effect on mycelial growth may also be determined by the species (Vainio et al, 2012;Hyder et al, 2013), isolate (Hunst et al, 1986;Rodríguez-García et al, 2014;Yu et al, 2015) temperature (Vainio et al, 2010;Bryner & Rigling, 2011;Romeralo et al, 2012;Zamora et al, 2016) or the type of mycovirus (Cryphonectria parasitica CHV1, mycovirus hypovirulences).…”

Section: Effect Of Fusarium Mycoviruses On Colony Growthmentioning

confidence: 99%

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

et al. 2018

View full text Add to dashboard Cite

Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1 and 2-2 (FcMV1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis.Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses).Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61).Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required Additional keywords: pine pitch canker; hypovirulence; biological control; forest pathology.

show abstract

“…They lack true virions, and have a (+) ssRNA genome of approximately 2.5 kb (Boland, 2004). Mitoviruses have been recorded in several phytopathogenic fungi such as Cryphonectria parasitica (Polashock and Hillman, 1994;Polashock et al, 1997), Ophiostoma novo-ulmi (Brasier, 1983;Rogers et al, 1987), Sclerotina homoeocarpa (Deng et al, 2003;Deng and Boland, 2004), Helicobasidium mompa (Osaki et al, 2005), Chalara elegans (Park et al, 2006) and Botrytis cinerea (Castro et al, 2003;Wu et al, 2007). In most cases, the presence of mitoviruses is associated with reduction of fungal pathogenicity (Ghabrial and Suzuki, 2009;Wu et al, 2010).…”

Section: Resumen Efecto De Posibles Mitovirus En El Crecimiento In VImentioning

confidence: 99%

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

View full text Add to dashboard Cite

Mitoviruses have been found in several forest pathogens (i.e. Cryphonectria parasitica, Gremmeniella abietina), and because they have been shown to reduce the virulence of host fungi there is a growing interest in studying their use as a biocontrol. This study was carried out to test the effect of temperature (5°C, 15°C, 25°C and 35°C), pH (4, 5, 7 and 9) and osmotic potential (-0.6, -1.2, -1.8 and -2.4 MPa) on the mycelial growth of seven G. abietina isolates under controlled laboratory conditions. Four of the isolates hosted mitoviruses and three of them did not. During the experiment, mycelial growth was recorded every week for a period of 8 weeks. Results showed no differences in growth behavior between mitovirus infected and non-infected isolates when placed under different pH modifications. However, the mitovirus-infected isolates presented larger mycelial growth than the mitovirus-free ones when at the fungi's optimal growing temperature of 15°C. When growing at certain osmotic potentials (-0.6 and -1.8 MPa) a reduction in growth of the mitovirus-infected isolates was observed. The results of this experiment suggest that mycelial growth among non-infected isolates and isolates naturally infected by mitovirus vary under different culture conditions, thus providing further insight into the effects of mitovirus on Gremmeniella abietina isolates.Key words: mitoviruses; Scleroderris canker; in vitro; biological control; Gremmeniella abietina; dsRNA. Resumen Efecto de posibles mitovirus en el crecimiento in vitro de aislados de Gremmeniella abietina bajo diferentes condiciones de laboratorioLos mitovirus son virus exclusivamente fúngicos que han sido aislados de algunos patógenos forestales (i.e. Cryphonectria parasitica, Gremmeniella abietina) y ya que pueden reducir la virulencia del hongo existe un creciente interés por su posible papel como agentes de control biológico. Se ha llevado a cabo un estudio para evaluar el efecto de la temperatura (5°C, 15°C, 25°C y 35°C), el pH (4, 5, 7 y 9) y el potencial osmótico (-0.6, -1.2, -1.8, -2.4 MPa) en el crecimiento micelial de siete aislados de G. abietina bajo condiciones controladas de laboratorio. Cuatro de los aislados albergaban mitovirus y tres de ellos no. Durante el experimento, el crecimiento micelial fue registrado semanalmente hasta completar 8 mediciones. Los aislados infectados con mitovirus presentaron mayor crecimiento micelial que los no infectados a la temperatura de crecimiento óptimo del hongo de 15°C. No se observaron efectos de la presencia de mitovirus entre los aislados infectados y los no infectados en los tratamientos de modificación del pH. Cuando se modificaron los potenciales osmóticos se observó una reducción del crecimiento micelial de los aislados infectados con mitovirus en los potenciales osmóticos de -0.6 y -1.8 MPa. Los resultados de este experimento sugieren que la presencia de los mitovirus afecta al crecimiento micelial del hongo bajo distintas condiciones de laboratorio. Este estudio proporciona un conocimiento más prof...

show abstract

A double-stranded RNA mycovirus confers hypovirulence-associated traits toBotrytis cinerea

Cited by 94 publications

References 22 publications

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

Contact Info

Product

Resources

About