A double SORLIP1 element is required for high light induction of ELIP genes in Arabidopsis thaliana

Alvarez-Canterbury, Ana M. Rus; Flores, Daisy Janette; Keymanesh, Keykhosrow; To, Kevin; Brusslan, Judy A.

doi:10.1007/s11103-013-0130-4

Cited by 9 publications

(10 citation statements)

References 42 publications

(38 reference statements)

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“…In our study, single SORLIP enhanced the luciferase activity compared with dark control and, surprisingly, the duplicated SORLIP increased the luciferase activity enormously. This result supports the result of Alvarez‐Canterbury et al that a double SORLIP1 is required for HL induction of ELIP genes in Arabidopsis . Because duplicated SORLIP is a stronger synthetic promoter than the native 400 bp LIP promoter fragment (Fig 5C), it is highly useful for overexpression of protein(s) under HL.…”

Section: Discussionsupporting

confidence: 91%

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Introducing Dunaliella LIP promoter containing light‐inducible motifs improves transgenic expression in Chlamydomonas reinhardtii

Baek

Lee

Nam

et al. 2016

Biotechnology Journal

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Promoter of the light-inducible protein gene (LIP) of Dunaliella was recently isolated in our laboratory. The aim of this work is to find the light-inducible motif in the Dunaliella LIP promoter and verify its regulatory motif with a Gaussia luciferase reporter gene transformed in Chlamydomonas reinhardtii. 400 bp upstream to the translational start site of the Dunaliella LIP gene was gradually truncated and analyzed for the luciferase expression. Furthermore, this promoter comprising duplicated or triplicated light-responsive motifs was tested for its augmentation of light response. Two putative light-responsive motifs, GT-1 binding motif and sequences over-represented in light-repressed promoters (SORLIP) located in the 200 bp LIP promoter fragment were analyzed for their light responsibility. It is turned out that SORLIP was responsible for the light-inducible activity. With the copy number of SORLIP up to three showed stronger high light response compared with the native LIP promoter fragment. Therefore, we found a light-responsive DNA motif operating in Chlamydomonas and confirm a synthetic promoter including this motif displayed light inducibility in heterologously transformed green algae for the first time. This light-inducible expression system will be applied to various area of algal research including algal biotechnology.

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Section: Discussionsupporting

confidence: 91%

“…GT‐1 binding motif is also activated by phytochrome, the red/far‐red light photoreceptor , and salt . Interestingly, the LIP promoter is activated by high salt . Therefore it might be interesting to test the salt stress for the GT binding motif.…”

Section: Discussionmentioning

confidence: 99%

Introducing Dunaliella LIP promoter containing light‐inducible motifs improves transgenic expression in Chlamydomonas reinhardtii

Baek

Lee

Nam

et al. 2016

Biotechnology Journal

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“…Photosynthesis genes were observed to be downregulated between these stages (Fig. 8 ), thus activation via these elements may be reduced during the studied period [ 47 , 49 , 50 ]. The ABFs element is enriched in promoters of genes declining in expression throughout the cotyledon timecourse, and is a G-box variant implicated in ABA response and targeted by bZIP transcription factors [ 51 ] (Fig.…”

Section: Resultsmentioning

confidence: 99%

Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons

Brown

Hudson

2015

BMC Plant Biol

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BackgroundImmediately following germination, the developing soybean seedling relies on the nutrient reserves stored in the cotyledons to sustain heterotrophic growth. During the seed filling period, developing seeds rely on the transport of nutrients from the trifoliate leaves. In soybean, both cotyledons and leaves develop the capacity for photosynthesis, and subsequently senesce and abscise once their function has ended. Before this occurs, the nutrients they contain are mobilized and transported to other parts of the plant. These processes are carefully orchestrated by genetic regulation throughout the development of the leaf or cotyledon.ResultsTo identify genes involved in the processes of leaf or cotyledon development and senescence in soybean, we used RNA-seq to profile multiple stages of cotyledon and leaf tissues. Differentially expressed genes between stages of leaf or cotyledon development were determined, major patterns of gene expression were defined, and shared genes were identified. Over 38,000 transcripts were expressed during the course of leaf and cotyledon development. Of those transcripts, 5,000 were expressed in a tissue specific pattern. Of the genes that were differentially expressed between both later stage tissues, 90 % had the same direction of change, suggesting that the mechanisms of senescence are conserved between tissues. Analysis of the enrichment of biological functions within genes sharing common expression profiles highlights the main processes occurring within these defined temporal windows of leaf and cotyledon development. Over 1,000 genes were identified with predicted regulatory functions that may have a role in control of leaf or cotyledon senescence.ConclusionsThe process of leaf and cotyledon development can be divided into distinct stages characterized by the expression of specific gene sets. The importance of the WRKY, NAC, and GRAS family transcription factors as major regulators of plant senescence is confirmed for both soybean leaf and cotyledon tissues. These results help validate functional annotation for soybean genes and promoters.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0553-y) contains supplementary material, which is available to authorized users.

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“…Both genes are also activated by HL in part through a blue light receptor, CRYPTOCHROME1 (CRY1; Kleine et al, 2007). Promoter analyses have demonstrated that regulatory elements found in ELIP1 and ELIP2 (designated as ELIP1 double SORLIP1 [novel sequences overrepresented in light-induced promoters] and ELIP2 double SORLIP1) are indispensable for activation by HL (Rus Alvarez-Canterbury et al, 2014).…”

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confidence: 99%

The Responses of Arabidopsis Early Light-Induced Protein2 to Ultraviolet B, High Light, and Cold Stress Are Regulated by a Transcriptional Regulatory Unit Composed of Two Elements

Hayami

Sakai²,

Kimura

et al. 2015

Plant Physiol.

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A double SORLIP1 element is required for high light induction of ELIP genes in Arabidopsis thaliana

Cited by 9 publications

References 42 publications

Introducing Dunaliella LIP promoter containing light‐inducible motifs improves transgenic expression in Chlamydomonas reinhardtii

Introducing Dunaliella LIP promoter containing light‐inducible motifs improves transgenic expression in Chlamydomonas reinhardtii

Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons

The Responses of Arabidopsis Early Light-Induced Protein2 to Ultraviolet B, High Light, and Cold Stress Are Regulated by a Transcriptional Regulatory Unit Composed of Two Elements

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